SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION

Objective: To study Simvastatin induced, qualitative histomorphological changes in skeletal muscle fibers of rats and find protective effect of Formoterol co-administration in Simvastatin induced myopathy. Study Design: Laboratory based experimental randomized controlled trial. Place and Durat...

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Autores principales:	Abdullah Qamar, Shoaib Naiyar Hashmi, Fareeha Mushtaq
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	formoterol myopathy skeletal muscle Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ac8c16b1dc9d4632a1a3972153d860a0
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spelling	oai:doaj.org-article:ac8c16b1dc9d4632a1a3972153d860a02021-11-12T03:43:38ZSIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION0030-96482411-8842https://doaj.org/article/ac8c16b1dc9d4632a1a3972153d860a02019-06-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3039/2273https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To study Simvastatin induced, qualitative histomorphological changes in skeletal muscle fibers of rats and find protective effect of Formoterol co-administration in Simvastatin induced myopathy. Study Design: Laboratory based experimental randomized controlled trial. Place and Duration of Study: Study was conducted at the department of Anatomy, Army Medical College Rawalpindi in collaboration with National Institute of Health (NIH) Islamabad and Armed forces institute of Pathology (AFIP) Rawalpindi, from Jan 2015 to Jun 2016. Material and Methods: Adult male Sprague-dawley rats were procured from NIH Islamabad. Their average approximate age was 70-80 days with weight as 250 ± 50 grams. The animals were randomly selected and divided into three groups. Group A was the control. Each rat of group B received Simvastatin dissolved in distilled water, by oral gavage (60mg/kg/day) once daily, for 12 weeks. Animals of Group C received simvastatin dissolved in distilled water (60mg/kg/day) once daily plus formoterol dissolved in distilled water (3μg/kg/day) once daily for 12 weeks. Both were administered with the help of oral gavage. The animals were sacrificed after three months of the experimental period. Extensor digitorum longus (EDL) tendon was isolated and dissected out. Tissue processing was done on the EDL muscle followed by Hematoxylin and Eosin staining. Fiber variability, inflammation, necrosis, shape of nuclei and location of nuclei were assessed per high power field in each specimen of all the groups. Results: Examination of H&E stained sections of the extensor digitorum longus muscle of the control group revealed the normal structure of skeletal muscle. Fiber variability, inflammation and necrosis were significantly higher in group B as compared to the control group A. Group C showed significantly decrease in myofiber variability, inflammation and necrosis in myofibers as compared to group B. Conclusion: Simvastatin induced the histomorphological changes in the skeletal muscle of experimental rats by increasing myofiber variability, inflammation and necrosis. Formoterol co-administration minimized the simvastatin induced myopathy by significantly decreasing myofiber variability, inflammation and necrosis.Abdullah QamarShoaib Naiyar HashmiFareeha MushtaqArmy Medical College Rawalpindiarticleformoterolmyopathyskeletal muscleMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 3, Pp 605-610 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	formoterol myopathy skeletal muscle Medicine R Medicine (General) R5-920
spellingShingle	formoterol myopathy skeletal muscle Medicine R Medicine (General) R5-920 Abdullah Qamar Shoaib Naiyar Hashmi Fareeha Mushtaq SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION
description	Objective: To study Simvastatin induced, qualitative histomorphological changes in skeletal muscle fibers of rats and find protective effect of Formoterol co-administration in Simvastatin induced myopathy. Study Design: Laboratory based experimental randomized controlled trial. Place and Duration of Study: Study was conducted at the department of Anatomy, Army Medical College Rawalpindi in collaboration with National Institute of Health (NIH) Islamabad and Armed forces institute of Pathology (AFIP) Rawalpindi, from Jan 2015 to Jun 2016. Material and Methods: Adult male Sprague-dawley rats were procured from NIH Islamabad. Their average approximate age was 70-80 days with weight as 250 ± 50 grams. The animals were randomly selected and divided into three groups. Group A was the control. Each rat of group B received Simvastatin dissolved in distilled water, by oral gavage (60mg/kg/day) once daily, for 12 weeks. Animals of Group C received simvastatin dissolved in distilled water (60mg/kg/day) once daily plus formoterol dissolved in distilled water (3μg/kg/day) once daily for 12 weeks. Both were administered with the help of oral gavage. The animals were sacrificed after three months of the experimental period. Extensor digitorum longus (EDL) tendon was isolated and dissected out. Tissue processing was done on the EDL muscle followed by Hematoxylin and Eosin staining. Fiber variability, inflammation, necrosis, shape of nuclei and location of nuclei were assessed per high power field in each specimen of all the groups. Results: Examination of H&E stained sections of the extensor digitorum longus muscle of the control group revealed the normal structure of skeletal muscle. Fiber variability, inflammation and necrosis were significantly higher in group B as compared to the control group A. Group C showed significantly decrease in myofiber variability, inflammation and necrosis in myofibers as compared to group B. Conclusion: Simvastatin induced the histomorphological changes in the skeletal muscle of experimental rats by increasing myofiber variability, inflammation and necrosis. Formoterol co-administration minimized the simvastatin induced myopathy by significantly decreasing myofiber variability, inflammation and necrosis.
format	article
author	Abdullah Qamar Shoaib Naiyar Hashmi Fareeha Mushtaq
author_facet	Abdullah Qamar Shoaib Naiyar Hashmi Fareeha Mushtaq
author_sort	Abdullah Qamar
title	SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION
title_short	SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION
title_full	SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION
title_fullStr	SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION
title_full_unstemmed	SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION
title_sort	simvastatin induced histomorphological changes in skeletal muscle fiber of rats and protective effect of formoterol coadministration
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/ac8c16b1dc9d4632a1a3972153d860a0
work_keys_str_mv	AT abdullahqamar simvastatininducedhistomorphologicalchangesinskeletalmusclefiberofratsandprotectiveeffectofformoterolcoadministration AT shoaibnaiyarhashmi simvastatininducedhistomorphologicalchangesinskeletalmusclefiberofratsandprotectiveeffectofformoterolcoadministration AT fareehamushtaq simvastatininducedhistomorphologicalchangesinskeletalmusclefiberofratsandprotectiveeffectofformoterolcoadministration
_version_	1718431309123026944

SIMVASTATIN INDUCED HISTOMORPHOLOGICAL CHANGES IN SKELETAL MUSCLE FIBER OF RATS AND PROTECTIVE EFFECT OF FORMOTEROL COADMINISTRATION

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