Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma

Abstract Hypopharyngeal squamous cell carcinoma (HSCC) accounts 95% of hypopharyngeal cancer, which is characterized by high early metastasis rate and poor prognosis. It is reported that circular RNA is involved in the occurrence and development of cancer; however, the role of circRNA in hypopharyng...

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Autores principales: Ce Li, Wenming Li, Shengda Cao, Jianing Xu, Ye Qian, Xinliang Pan, Dapeng Lei, Dongmin Wei
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Lenguaje:EN
Publicado: Nature Publishing Group 2021
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Acceso en línea:https://doaj.org/article/ac8edfec23834e26a00af8c7f9142f91
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spelling oai:doaj.org-article:ac8edfec23834e26a00af8c7f9142f912021-11-14T12:06:42ZCirc_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma10.1038/s41419-021-04346-82041-4889https://doaj.org/article/ac8edfec23834e26a00af8c7f9142f912021-11-01T00:00:00Zhttps://doi.org/10.1038/s41419-021-04346-8https://doaj.org/toc/2041-4889Abstract Hypopharyngeal squamous cell carcinoma (HSCC) accounts 95% of hypopharyngeal cancer, which is characterized by high early metastasis rate and poor prognosis. It is reported that circular RNA is involved in the occurrence and development of cancer; however, the role of circRNA in hypopharyngeal cancer has little been investigated. We performed hypopharyngeal carcinoma circRNA microarray and qRT-PCR verification. The results showed circ_0058106 expression level was significantly upregulated in tumor tissues than in corresponding normal tissues. We found that circ_0058106 upregulation promoted proliferation, migration and invasion of HSCC cells, while knockdown of circ_0058106 inhibited proliferation, migration and invasion of HSCC cells both in vitro and in vivo. Bioinformatics predicted circ_0058106 may interact with miR-185-3p. We verified circ_0058106 directly bound miR-185-3p and downregulated miR-185-3p expression by using dual-luciferase reporter assay and qRT-PCR. Moreover, we proved circ_0058106 promoted HSCC cells tumorigenesis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway via miR-185-3p. In conclusion, our findings firstly confirmed the carcinogenic effect of circ_0058106 in promoting HSCC cells tumorigenesis, metastasis, invasion and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through sponging miR-185-3p, indicating that circ_0058106 may be a new therapeutic target and prognostic marker for HSCC.Ce LiWenming LiShengda CaoJianing XuYe QianXinliang PanDapeng LeiDongmin WeiNature Publishing GrouparticleCytologyQH573-671ENCell Death and Disease, Vol 12, Iss 11, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Cytology
QH573-671
spellingShingle Cytology
QH573-671
Ce Li
Wenming Li
Shengda Cao
Jianing Xu
Ye Qian
Xinliang Pan
Dapeng Lei
Dongmin Wei
Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma
description Abstract Hypopharyngeal squamous cell carcinoma (HSCC) accounts 95% of hypopharyngeal cancer, which is characterized by high early metastasis rate and poor prognosis. It is reported that circular RNA is involved in the occurrence and development of cancer; however, the role of circRNA in hypopharyngeal cancer has little been investigated. We performed hypopharyngeal carcinoma circRNA microarray and qRT-PCR verification. The results showed circ_0058106 expression level was significantly upregulated in tumor tissues than in corresponding normal tissues. We found that circ_0058106 upregulation promoted proliferation, migration and invasion of HSCC cells, while knockdown of circ_0058106 inhibited proliferation, migration and invasion of HSCC cells both in vitro and in vivo. Bioinformatics predicted circ_0058106 may interact with miR-185-3p. We verified circ_0058106 directly bound miR-185-3p and downregulated miR-185-3p expression by using dual-luciferase reporter assay and qRT-PCR. Moreover, we proved circ_0058106 promoted HSCC cells tumorigenesis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway via miR-185-3p. In conclusion, our findings firstly confirmed the carcinogenic effect of circ_0058106 in promoting HSCC cells tumorigenesis, metastasis, invasion and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through sponging miR-185-3p, indicating that circ_0058106 may be a new therapeutic target and prognostic marker for HSCC.
format article
author Ce Li
Wenming Li
Shengda Cao
Jianing Xu
Ye Qian
Xinliang Pan
Dapeng Lei
Dongmin Wei
author_facet Ce Li
Wenming Li
Shengda Cao
Jianing Xu
Ye Qian
Xinliang Pan
Dapeng Lei
Dongmin Wei
author_sort Ce Li
title Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma
title_short Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma
title_full Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma
title_fullStr Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma
title_full_unstemmed Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma
title_sort circ_0058106 promotes proliferation, metastasis and emt process by regulating wnt2b/β-catenin/c-myc pathway through mir-185-3p in hypopharyngeal squamous cell carcinoma
publisher Nature Publishing Group
publishDate 2021
url https://doaj.org/article/ac8edfec23834e26a00af8c7f9142f91
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AT wenmingli circ0058106promotesproliferationmetastasisandemtprocessbyregulatingwnt2bbcatenincmycpathwaythroughmir1853pinhypopharyngealsquamouscellcarcinoma
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AT yeqian circ0058106promotesproliferationmetastasisandemtprocessbyregulatingwnt2bbcatenincmycpathwaythroughmir1853pinhypopharyngealsquamouscellcarcinoma
AT xinliangpan circ0058106promotesproliferationmetastasisandemtprocessbyregulatingwnt2bbcatenincmycpathwaythroughmir1853pinhypopharyngealsquamouscellcarcinoma
AT dapenglei circ0058106promotesproliferationmetastasisandemtprocessbyregulatingwnt2bbcatenincmycpathwaythroughmir1853pinhypopharyngealsquamouscellcarcinoma
AT dongminwei circ0058106promotesproliferationmetastasisandemtprocessbyregulatingwnt2bbcatenincmycpathwaythroughmir1853pinhypopharyngealsquamouscellcarcinoma
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