IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS

Abstract. Interactions between B1, B2 and bone marrow dendritic cells (DCs) of CBA mice were investigated in the course of immune response to T-independent antigen type 2 (TI 2 Ag) in model system. Splenocytes of Xid-mice CBA/N were used as “fillers”. It was shown that DCs augmented viability of B1...

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Autores principales:	M. V. Gavrilova, I. N. Chernyshova, D. A. Khochenkov, E. V. Sidorova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	b1 cells th lymphocytes тн2 antigens в2 lymphocytes dendritic cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ac93bd366d8c43208c31d0931793201e
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spelling	oai:doaj.org-article:ac93bd366d8c43208c31d0931793201e2021-11-18T08:03:43ZIN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS1563-06252313-741X10.15789/1563-0625-2013-4-325-334https://doaj.org/article/ac93bd366d8c43208c31d0931793201e2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/643https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Interactions between B1, B2 and bone marrow dendritic cells (DCs) of CBA mice were investigated in the course of immune response to T-independent antigen type 2 (TI 2 Ag) in model system. Splenocytes of Xid-mice CBA/N were used as “fillers”. It was shown that DCs augmented viability of B1 and B2 lymphocytes. Functional activity of the B1 and B2 lymphocytes was determined by ELISPOT method, as relative numbers of antibody- and immunoglobulin-forming cells (AFCs and IFCs, respectively) detectable after four days in culture. An increased immune response to TI 2 Ag in presence of DCs was observed mainly for B2 subpopulation, containing MZ-B lymphocytes as well. Specific effect of DC upon B2 cell subpopulation was confirmed by detection of direct in vitro interactions of DCs with T and B lymphocytes. DCs cultivated with B2 and T lymphocytes, used as fillers, decreased the numbers of AFCs and IFCs, irrespectively of absence or presence of TI-2 Ags. A decrease in AFC and IFC numbers in the cultures with B1 cells was evident in presence of the Ags only. It was shown that the inhibitory effect of DCs depended on their contact interactions. Separate cultures of B1 and B2 cells with DC under transwell conditions resulted into occurence of AFC and IFC in amounts similar to those generated in vitro without DCs.M. V. GavrilovaI. N. ChernyshovaD. A. KhochenkovE. V. SidorovaSPb RAACIarticleb1 cellsth lymphocytesтн2 antigensв2 lymphocytesdendritic cellsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 4, Pp 325-334 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	b1 cells th lymphocytes тн2 antigens в2 lymphocytes dendritic cells Immunologic diseases. Allergy RC581-607
spellingShingle	b1 cells th lymphocytes тн2 antigens в2 lymphocytes dendritic cells Immunologic diseases. Allergy RC581-607 M. V. Gavrilova I. N. Chernyshova D. A. Khochenkov E. V. Sidorova IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS
description	Abstract. Interactions between B1, B2 and bone marrow dendritic cells (DCs) of CBA mice were investigated in the course of immune response to T-independent antigen type 2 (TI 2 Ag) in model system. Splenocytes of Xid-mice CBA/N were used as “fillers”. It was shown that DCs augmented viability of B1 and B2 lymphocytes. Functional activity of the B1 and B2 lymphocytes was determined by ELISPOT method, as relative numbers of antibody- and immunoglobulin-forming cells (AFCs and IFCs, respectively) detectable after four days in culture. An increased immune response to TI 2 Ag in presence of DCs was observed mainly for B2 subpopulation, containing MZ-B lymphocytes as well. Specific effect of DC upon B2 cell subpopulation was confirmed by detection of direct in vitro interactions of DCs with T and B lymphocytes. DCs cultivated with B2 and T lymphocytes, used as fillers, decreased the numbers of AFCs and IFCs, irrespectively of absence or presence of TI-2 Ags. A decrease in AFC and IFC numbers in the cultures with B1 cells was evident in presence of the Ags only. It was shown that the inhibitory effect of DCs depended on their contact interactions. Separate cultures of B1 and B2 cells with DC under transwell conditions resulted into occurence of AFC and IFC in amounts similar to those generated in vitro without DCs.
format	article
author	M. V. Gavrilova I. N. Chernyshova D. A. Khochenkov E. V. Sidorova
author_facet	M. V. Gavrilova I. N. Chernyshova D. A. Khochenkov E. V. Sidorova
author_sort	M. V. Gavrilova
title	IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS
title_short	IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS
title_full	IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS
title_fullStr	IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS
title_full_unstemmed	IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS
title_sort	in vitro cellular interactions during immune response to t cellindependent type 2 antigens
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/ac93bd366d8c43208c31d0931793201e
work_keys_str_mv	AT mvgavrilova invitrocellularinteractionsduringimmuneresponsetotcellindependenttype2antigens AT inchernyshova invitrocellularinteractionsduringimmuneresponsetotcellindependenttype2antigens AT dakhochenkov invitrocellularinteractionsduringimmuneresponsetotcellindependenttype2antigens AT evsidorova invitrocellularinteractionsduringimmuneresponsetotcellindependenttype2antigens
_version_	1718422441074622464

IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS

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