Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice

Abstract Lupus nephritis is a crucial complication of systemic lupus erythematosus. In this study, we investigated the roles of mouse natural killer T (NKT) cells in lupus nephritis. From 24 weeks of age, NZB/NZW F1 mice were injected with alpha-galactosylceramide (α-GalCer) or vehicle once a week f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takahiro Uchida, Hiroyuki Nakashima, Akira Yamagata, Seigo Ito, Takuya Ishikiriyama, Masahiro Nakashima, Shuhji Seki, Hiroo Kumagai, Naoki Oshima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac99edb139cf49b3ad842ac3b0879ae8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac99edb139cf49b3ad842ac3b0879ae8
record_format	dspace
spelling	oai:doaj.org-article:ac99edb139cf49b3ad842ac3b0879ae82021-12-02T15:08:55ZRepeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice10.1038/s41598-018-26470-w2045-2322https://doaj.org/article/ac99edb139cf49b3ad842ac3b0879ae82018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26470-whttps://doaj.org/toc/2045-2322Abstract Lupus nephritis is a crucial complication of systemic lupus erythematosus. In this study, we investigated the roles of mouse natural killer T (NKT) cells in lupus nephritis. From 24 weeks of age, NZB/NZW F1 mice were injected with alpha-galactosylceramide (α-GalCer) or vehicle once a week for four weeks. In the α-GalCer group, the levels of proteinuria and blood urea nitrogen were significantly lower than those in the vehicle group. The histological evaluation showed a decrease in glomerular immune complex deposits and an alleviation of podocyte injury. The proportion of NKT cells in the mononuclear cell (MNC) fraction in the α-GalCer group was significantly decreased in the liver, kidney, and spleen. The proliferation and cytokine production in α-GalCer-stimulated liver MNCs were markedly diminished in the α-GalCer group (anergy). The IFN-γ production in liver MNCs stimulated by concanavalin A or an anti-CD3 antibody did not differ between the two groups, whereas the IL-4 production was significantly lower in the α-GalCer group. In addition, the IgM production in CpG-oligodeoxynucleotide-stimulated spleen MNCs was significantly lower in the α-GalCer group. These results suggest that α-GalCer suppressed Th2 immune responses in NKT cells and B cell function, thereby slowing the progression of lupus nephritis.Takahiro UchidaHiroyuki NakashimaAkira YamagataSeigo ItoTakuya IshikiriyamaMasahiro NakashimaShuhji SekiHiroo KumagaiNaoki OshimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takahiro Uchida Hiroyuki Nakashima Akira Yamagata Seigo Ito Takuya Ishikiriyama Masahiro Nakashima Shuhji Seki Hiroo Kumagai Naoki Oshima Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
description	Abstract Lupus nephritis is a crucial complication of systemic lupus erythematosus. In this study, we investigated the roles of mouse natural killer T (NKT) cells in lupus nephritis. From 24 weeks of age, NZB/NZW F1 mice were injected with alpha-galactosylceramide (α-GalCer) or vehicle once a week for four weeks. In the α-GalCer group, the levels of proteinuria and blood urea nitrogen were significantly lower than those in the vehicle group. The histological evaluation showed a decrease in glomerular immune complex deposits and an alleviation of podocyte injury. The proportion of NKT cells in the mononuclear cell (MNC) fraction in the α-GalCer group was significantly decreased in the liver, kidney, and spleen. The proliferation and cytokine production in α-GalCer-stimulated liver MNCs were markedly diminished in the α-GalCer group (anergy). The IFN-γ production in liver MNCs stimulated by concanavalin A or an anti-CD3 antibody did not differ between the two groups, whereas the IL-4 production was significantly lower in the α-GalCer group. In addition, the IgM production in CpG-oligodeoxynucleotide-stimulated spleen MNCs was significantly lower in the α-GalCer group. These results suggest that α-GalCer suppressed Th2 immune responses in NKT cells and B cell function, thereby slowing the progression of lupus nephritis.
format	article
author	Takahiro Uchida Hiroyuki Nakashima Akira Yamagata Seigo Ito Takuya Ishikiriyama Masahiro Nakashima Shuhji Seki Hiroo Kumagai Naoki Oshima
author_facet	Takahiro Uchida Hiroyuki Nakashima Akira Yamagata Seigo Ito Takuya Ishikiriyama Masahiro Nakashima Shuhji Seki Hiroo Kumagai Naoki Oshima
author_sort	Takahiro Uchida
title	Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
title_short	Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
title_full	Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
title_fullStr	Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
title_full_unstemmed	Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
title_sort	repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ac99edb139cf49b3ad842ac3b0879ae8
work_keys_str_mv	AT takahirouchida repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT hiroyukinakashima repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT akirayamagata repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT seigoito repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT takuyaishikiriyama repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT masahironakashima repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT shuhjiseki repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT hirookumagai repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice AT naokioshima repeatedadministrationofalphagalactosylceramideamelioratesexperimentallupusnephritisinmice
_version_	1718387958380232704

Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice

Ejemplares similares