Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.

<h4>Background</h4>Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic enteritis in a wide range of animal species. In cattle, MAP causes a chronic disease called Johne's disease, or paratuberculosis, that is not treatable and the efficacy of vaccine control is controve...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Giulietta Minozzi, Laura Buggiotti, Alessandra Stella, Francesco Strozzi, Mario Luini, John L Williams
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac9b29b7a5b443b486ae4b6a880622e1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac9b29b7a5b443b486ae4b6a880622e1
record_format	dspace
spelling	oai:doaj.org-article:ac9b29b7a5b443b486ae4b6a880622e12021-12-02T20:20:47ZGenetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.1932-620310.1371/journal.pone.0011117https://doaj.org/article/ac9b29b7a5b443b486ae4b6a880622e12010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20559561/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic enteritis in a wide range of animal species. In cattle, MAP causes a chronic disease called Johne's disease, or paratuberculosis, that is not treatable and the efficacy of vaccine control is controversial. The clinical phase of the disease is characterised by diarrhoea, weight loss, drop in milk production and eventually death. Susceptibility to MAP infection is heritable with heritability estimates ranging from 0.06 to 0.10. There have been several studies over the last few years that have identified genetic loci putatively associated with MAP susceptibility, however, with the availability of genome-wide high density SNP maker panels it is now possible to carry out association studies that have higher precision.<h4>Methodology/principal findings</h4>The objective of the current study was to localize genes having an impact on Johne's disease susceptibility using the latest bovine genome information and a high density SNP panel (Illumina BovineSNP50 BeadChip) to perform a case/control, genome-wide association analysis. Samples from MAP case and negative controls were selected from field samples collected in 2007 and 2008 in the province of Lombardy, Italy. Cases were defined as animals serologically positive for MAP by ELISA. In total 966 samples were genotyped: 483 MAP ELISA positive and 483 ELISA negative. Samples were selected randomly among those collected from 119 farms which had at least one positive animal.<h4>Conclusion/significance</h4>THE ANALYSIS OF THE GENOTYPE DATA IDENTIFIED SEVERAL CHROMOSOMAL REGIONS ASSOCIATED WITH DISEASE STATUS: a region on chromosome 12 with high significance (P<5x10(-6)), while regions on chromosome 9, 11, and 12 had moderate significance (P<5x10(-5)). These results provide evidence for genetic loci involved in the humoral response to MAP. Knowledge of genetic variations related to susceptibility will facilitate the incorporation of this information into breeding programmes for the improvement of health status.Giulietta MinozziLaura BuggiottiAlessandra StellaFrancesco StrozziMario LuiniJohn L WilliamsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11117 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giulietta Minozzi Laura Buggiotti Alessandra Stella Francesco Strozzi Mario Luini John L Williams Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.
description	<h4>Background</h4>Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic enteritis in a wide range of animal species. In cattle, MAP causes a chronic disease called Johne's disease, or paratuberculosis, that is not treatable and the efficacy of vaccine control is controversial. The clinical phase of the disease is characterised by diarrhoea, weight loss, drop in milk production and eventually death. Susceptibility to MAP infection is heritable with heritability estimates ranging from 0.06 to 0.10. There have been several studies over the last few years that have identified genetic loci putatively associated with MAP susceptibility, however, with the availability of genome-wide high density SNP maker panels it is now possible to carry out association studies that have higher precision.<h4>Methodology/principal findings</h4>The objective of the current study was to localize genes having an impact on Johne's disease susceptibility using the latest bovine genome information and a high density SNP panel (Illumina BovineSNP50 BeadChip) to perform a case/control, genome-wide association analysis. Samples from MAP case and negative controls were selected from field samples collected in 2007 and 2008 in the province of Lombardy, Italy. Cases were defined as animals serologically positive for MAP by ELISA. In total 966 samples were genotyped: 483 MAP ELISA positive and 483 ELISA negative. Samples were selected randomly among those collected from 119 farms which had at least one positive animal.<h4>Conclusion/significance</h4>THE ANALYSIS OF THE GENOTYPE DATA IDENTIFIED SEVERAL CHROMOSOMAL REGIONS ASSOCIATED WITH DISEASE STATUS: a region on chromosome 12 with high significance (P<5x10(-6)), while regions on chromosome 9, 11, and 12 had moderate significance (P<5x10(-5)). These results provide evidence for genetic loci involved in the humoral response to MAP. Knowledge of genetic variations related to susceptibility will facilitate the incorporation of this information into breeding programmes for the improvement of health status.
format	article
author	Giulietta Minozzi Laura Buggiotti Alessandra Stella Francesco Strozzi Mario Luini John L Williams
author_facet	Giulietta Minozzi Laura Buggiotti Alessandra Stella Francesco Strozzi Mario Luini John L Williams
author_sort	Giulietta Minozzi
title	Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.
title_short	Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.
title_full	Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.
title_fullStr	Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.
title_full_unstemmed	Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.
title_sort	genetic loci involved in antibody response to mycobacterium avium ssp. paratuberculosis in cattle.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/ac9b29b7a5b443b486ae4b6a880622e1
work_keys_str_mv	AT giuliettaminozzi geneticlociinvolvedinantibodyresponsetomycobacteriumaviumsspparatuberculosisincattle AT laurabuggiotti geneticlociinvolvedinantibodyresponsetomycobacteriumaviumsspparatuberculosisincattle AT alessandrastella geneticlociinvolvedinantibodyresponsetomycobacteriumaviumsspparatuberculosisincattle AT francescostrozzi geneticlociinvolvedinantibodyresponsetomycobacteriumaviumsspparatuberculosisincattle AT marioluini geneticlociinvolvedinantibodyresponsetomycobacteriumaviumsspparatuberculosisincattle AT johnlwilliams geneticlociinvolvedinantibodyresponsetomycobacteriumaviumsspparatuberculosisincattle
_version_	1718374184613052416

Genetic loci involved in antibody response to Mycobacterium avium ssp. paratuberculosis in cattle.

Ejemplares similares