Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks

Abstract Reactive oxygen species (ROS) are key pathological signals expressed in inflammatory diseases such as cancer, ischemic conditions and atherosclerosis. An ideal drug delivery system should not only be responsive to these signals but also should not elicit an unfavourable host response. This...

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Autores principales:	Gesmi Milcovich, Paolo Contessotto, Grazia Marsico, Siti Ismail, Abhay Pandit
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac9dc45dff104e34847ab710c4bda144
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spelling	oai:doaj.org-article:ac9dc45dff104e34847ab710c4bda1442021-12-02T15:05:11ZSynthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks10.1038/s41598-017-13744-y2045-2322https://doaj.org/article/ac9dc45dff104e34847ab710c4bda1442017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13744-yhttps://doaj.org/toc/2045-2322Abstract Reactive oxygen species (ROS) are key pathological signals expressed in inflammatory diseases such as cancer, ischemic conditions and atherosclerosis. An ideal drug delivery system should not only be responsive to these signals but also should not elicit an unfavourable host response. This study presents an innovative platform for drug delivery where a natural/synthetic composite system composed of collagen type I and a synthesized polythioether, ensures a dual stimuli-responsive behaviour. Collagen type I is an extracellular matrix constituent protein, responsive to matrix metalloproteinases (MMP) cleavage per se. Polythioethers are stable synthetic polymers characterized by the presence of sulphur, which undergoes a ROS-responsive swelling switch. A polythioether was synthesised, functionalized and tested for cytotoxicity. Optimal conditions to fabricate a composite natural/synthetic hollow sphere construct were optimised by a template-based method. Collagen-polythioether hollow spheres were fabricated, revealing uniform size and ROS-triggered nanoporation features. Cellular metabolic activity of H9C2 cardiomyoblasts remained unaffected upon exposure to the spheres. Our natural/synthetic hollow microspheres exhibit the potential for use as a pathological stimuli-responsive reservoir system for applications in inflammatory diseases.Gesmi MilcovichPaolo ContessottoGrazia MarsicoSiti IsmailAbhay PanditNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gesmi Milcovich Paolo Contessotto Grazia Marsico Siti Ismail Abhay Pandit Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks
description	Abstract Reactive oxygen species (ROS) are key pathological signals expressed in inflammatory diseases such as cancer, ischemic conditions and atherosclerosis. An ideal drug delivery system should not only be responsive to these signals but also should not elicit an unfavourable host response. This study presents an innovative platform for drug delivery where a natural/synthetic composite system composed of collagen type I and a synthesized polythioether, ensures a dual stimuli-responsive behaviour. Collagen type I is an extracellular matrix constituent protein, responsive to matrix metalloproteinases (MMP) cleavage per se. Polythioethers are stable synthetic polymers characterized by the presence of sulphur, which undergoes a ROS-responsive swelling switch. A polythioether was synthesised, functionalized and tested for cytotoxicity. Optimal conditions to fabricate a composite natural/synthetic hollow sphere construct were optimised by a template-based method. Collagen-polythioether hollow spheres were fabricated, revealing uniform size and ROS-triggered nanoporation features. Cellular metabolic activity of H9C2 cardiomyoblasts remained unaffected upon exposure to the spheres. Our natural/synthetic hollow microspheres exhibit the potential for use as a pathological stimuli-responsive reservoir system for applications in inflammatory diseases.
format	article
author	Gesmi Milcovich Paolo Contessotto Grazia Marsico Siti Ismail Abhay Pandit
author_facet	Gesmi Milcovich Paolo Contessotto Grazia Marsico Siti Ismail Abhay Pandit
author_sort	Gesmi Milcovich
title	Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks
title_short	Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks
title_full	Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks
title_fullStr	Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks
title_full_unstemmed	Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks
title_sort	synthetic/ecm-inspired hybrid platform for hollow microcarriers with ros-triggered nanoporation hallmarks
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ac9dc45dff104e34847ab710c4bda144
work_keys_str_mv	AT gesmimilcovich syntheticecminspiredhybridplatformforhollowmicrocarrierswithrostriggerednanoporationhallmarks AT paolocontessotto syntheticecminspiredhybridplatformforhollowmicrocarrierswithrostriggerednanoporationhallmarks AT graziamarsico syntheticecminspiredhybridplatformforhollowmicrocarrierswithrostriggerednanoporationhallmarks AT sitiismail syntheticecminspiredhybridplatformforhollowmicrocarrierswithrostriggerednanoporationhallmarks AT abhaypandit syntheticecminspiredhybridplatformforhollowmicrocarrierswithrostriggerednanoporationhallmarks
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Synthetic/ECM-inspired hybrid platform for hollow microcarriers with ROS-triggered nanoporation hallmarks

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