The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture

Background: Spermatogenesis is a programmed route for germ cell proliferation and differentiation that can produce abundant numbers of spermatozoa. The antioxidants play a vital role in decreasing oxidative stress production in cells; therefore, the extraction of plants with antioxidant property can...

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Autores principales:	Shirin Barati, Mansoureh Movahedin
Formato:	article
Lenguaje:	EN
Publicado:	Tabriz University of Medical Sciences 2021
Materias:	stem cells oxidative stress antioxidant calligonum comosum apoptosis Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/aca0e1da2a21418590ab3ba40f2d73d9
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spelling	oai:doaj.org-article:aca0e1da2a21418590ab3ba40f2d73d92021-11-29T05:52:46ZThe Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture2383-288610.34172/PS.2021.20https://doaj.org/article/aca0e1da2a21418590ab3ba40f2d73d92021-12-01T00:00:00Zhttps://ps.tbzmed.ac.ir/PDF/ps-27-521.pdfhttps://doaj.org/toc/2383-2886Background: Spermatogenesis is a programmed route for germ cell proliferation and differentiation that can produce abundant numbers of spermatozoa. The antioxidants play a vital role in decreasing oxidative stress production in cells; therefore, the extraction of plants with antioxidant property can prevent cell damage. In the present study, antioxidant effects of Calligonum extract on proliferation and colonization rate of spermatogonial cells were assessed. Methods: After isolation and culturing of spermatogonial stem cells (SSCs) on neonatal mice (4-5 days old) and identification by PLZF and Oct4 markers, the therapeutic effect of Calligonum comosum extract on cells treated with H2O2 was measured. The cultured cells were divided into four groups: Control, Calligonum, H2O2 and Calligonum + H2O2 groups. Induced oxidative stress cells were treated with 10 μg/ml extract for 3 weeks. Reactive oxygen species (ROS) levels were assessed by the flow cytometry, and proliferation and total antioxidant capacity (TAC) were evaluated by cell count and ferric reducing ability of plasma (FRAP) assay, respectively. Also, the apoptosis rate was measured with P53 and Bax genes by the real- time PCR method. Results: After three-week treatment, ROS level was significantly lower in the Calligonum group than in the H2O2 group. Antioxidants levels were significantly higher in Calligonum group than in the H2O2 group (P≤0.05). There was also a strong inverse relationship between the two groups. Proliferation and colonization rate were significantly higher in Calligonum + H2O2 group than in H2O2 group (P≤0.05). Finally, the results suggested that P53 and Bax expression decreased in Calligonum + H2O2 group compared to H2O2 group. Conclusion: The results of present study revealed that 30 μM doses of H2O2 increased oxidative stress and apoptosis on the one hand and decreased proliferation of SSCs on the other hand. Asa plant with antioxidant effect, Calligonum could reduce the level of ROS and apoptosis, and increase proliferation, colonization rate and TAC.Shirin BaratiMansoureh MovahedinTabriz University of Medical Sciencesarticlestem cellsoxidative stressantioxidantcalligonum comosumapoptosisPharmacy and materia medicaRS1-441ENPharmaceutical Sciences, Vol 27, Iss 4, Pp 521-527 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	stem cells oxidative stress antioxidant calligonum comosum apoptosis Pharmacy and materia medica RS1-441
spellingShingle	stem cells oxidative stress antioxidant calligonum comosum apoptosis Pharmacy and materia medica RS1-441 Shirin Barati Mansoureh Movahedin The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture
description	Background: Spermatogenesis is a programmed route for germ cell proliferation and differentiation that can produce abundant numbers of spermatozoa. The antioxidants play a vital role in decreasing oxidative stress production in cells; therefore, the extraction of plants with antioxidant property can prevent cell damage. In the present study, antioxidant effects of Calligonum extract on proliferation and colonization rate of spermatogonial cells were assessed. Methods: After isolation and culturing of spermatogonial stem cells (SSCs) on neonatal mice (4-5 days old) and identification by PLZF and Oct4 markers, the therapeutic effect of Calligonum comosum extract on cells treated with H2O2 was measured. The cultured cells were divided into four groups: Control, Calligonum, H2O2 and Calligonum + H2O2 groups. Induced oxidative stress cells were treated with 10 μg/ml extract for 3 weeks. Reactive oxygen species (ROS) levels were assessed by the flow cytometry, and proliferation and total antioxidant capacity (TAC) were evaluated by cell count and ferric reducing ability of plasma (FRAP) assay, respectively. Also, the apoptosis rate was measured with P53 and Bax genes by the real- time PCR method. Results: After three-week treatment, ROS level was significantly lower in the Calligonum group than in the H2O2 group. Antioxidants levels were significantly higher in Calligonum group than in the H2O2 group (P≤0.05). There was also a strong inverse relationship between the two groups. Proliferation and colonization rate were significantly higher in Calligonum + H2O2 group than in H2O2 group (P≤0.05). Finally, the results suggested that P53 and Bax expression decreased in Calligonum + H2O2 group compared to H2O2 group. Conclusion: The results of present study revealed that 30 μM doses of H2O2 increased oxidative stress and apoptosis on the one hand and decreased proliferation of SSCs on the other hand. Asa plant with antioxidant effect, Calligonum could reduce the level of ROS and apoptosis, and increase proliferation, colonization rate and TAC.
format	article
author	Shirin Barati Mansoureh Movahedin
author_facet	Shirin Barati Mansoureh Movahedin
author_sort	Shirin Barati
title	The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture
title_short	The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture
title_full	The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture
title_fullStr	The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture
title_full_unstemmed	The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture
title_sort	antioxidant effects of calligonum extract on oxidative stress in spermatogonial stem cells culture
publisher	Tabriz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/aca0e1da2a21418590ab3ba40f2d73d9
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The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture

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