Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico

Abstract Mastitis in cows is a major cause of economic losses and it is commonly associated with Staphylococcus aureus. Little is known about the S. aureus lineages causing mastitis in Mexican cattle. The aim of this study was to type S. aureus isolates causing mastitis in cows from the Comarca Lagu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Y. Mora-Hernández, E. Vera Murguía, J. Stinenbosch, P. Hernández Jauregui, Jan Maarten van Dijl, G. Buist
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/acb6bb1a0e8e4a93a1faba7eb2605a5d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:acb6bb1a0e8e4a93a1faba7eb2605a5d
record_format	dspace
spelling	oai:doaj.org-article:acb6bb1a0e8e4a93a1faba7eb2605a5d2021-12-02T16:36:11ZMolecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico10.1038/s41598-021-86453-22045-2322https://doaj.org/article/acb6bb1a0e8e4a93a1faba7eb2605a5d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86453-2https://doaj.org/toc/2045-2322Abstract Mastitis in cows is a major cause of economic losses and it is commonly associated with Staphylococcus aureus. Little is known about the S. aureus lineages causing mastitis in Mexican cattle. The aim of this study was to type S. aureus isolates causing mastitis in cows from the Comarca Lagunera region in Mexico in 2015–2016. Multi-locus variable number tandem repeat fingerprinting (MLVF) of 33 S. aureus isolates obtained from 210 milk samples revealed the MLVF clusters A (n = 1), B (n = 26), C (n = 5) and D (n = 1). Spa-typing showed that clusters A and B represent the spa-type t224, cluster C includes spa-types t3196 and t416, and cluster D represents spa-type t114. The different spa-types were mirrored by the masses of protein A bands as detected by Western blotting. Antimicrobial susceptibility testing showed that one isolate was susceptible to all antimicrobials tested, whereas all other strains were resistant only to benzylpenicillin. These findings show that only four S. aureus lineages, susceptible to most antimicrobials, were responsible for causing mastitis at the time of sampling. Lastly, many isolates carried the same small plasmid, designated pSAM1. The high prevalence of pSAM1 amongst the antimicrobial-susceptible isolates suggests an association with bovine colonization or mastitis rather than antimicrobial resistance.Y. Mora-HernándezE. Vera MurguíaJ. StinenboschP. Hernández JaureguiJan Maarten van DijlG. BuistNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Y. Mora-Hernández E. Vera Murguía J. Stinenbosch P. Hernández Jauregui Jan Maarten van Dijl G. Buist Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico
description	Abstract Mastitis in cows is a major cause of economic losses and it is commonly associated with Staphylococcus aureus. Little is known about the S. aureus lineages causing mastitis in Mexican cattle. The aim of this study was to type S. aureus isolates causing mastitis in cows from the Comarca Lagunera region in Mexico in 2015–2016. Multi-locus variable number tandem repeat fingerprinting (MLVF) of 33 S. aureus isolates obtained from 210 milk samples revealed the MLVF clusters A (n = 1), B (n = 26), C (n = 5) and D (n = 1). Spa-typing showed that clusters A and B represent the spa-type t224, cluster C includes spa-types t3196 and t416, and cluster D represents spa-type t114. The different spa-types were mirrored by the masses of protein A bands as detected by Western blotting. Antimicrobial susceptibility testing showed that one isolate was susceptible to all antimicrobials tested, whereas all other strains were resistant only to benzylpenicillin. These findings show that only four S. aureus lineages, susceptible to most antimicrobials, were responsible for causing mastitis at the time of sampling. Lastly, many isolates carried the same small plasmid, designated pSAM1. The high prevalence of pSAM1 amongst the antimicrobial-susceptible isolates suggests an association with bovine colonization or mastitis rather than antimicrobial resistance.
format	article
author	Y. Mora-Hernández E. Vera Murguía J. Stinenbosch P. Hernández Jauregui Jan Maarten van Dijl G. Buist
author_facet	Y. Mora-Hernández E. Vera Murguía J. Stinenbosch P. Hernández Jauregui Jan Maarten van Dijl G. Buist
author_sort	Y. Mora-Hernández
title	Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico
title_short	Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico
title_full	Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico
title_fullStr	Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico
title_full_unstemmed	Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico
title_sort	molecular typing and antimicrobial resistance profiling of 33 mastitis-related staphylococcus aureus isolates from cows in the comarca lagunera region of mexico
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/acb6bb1a0e8e4a93a1faba7eb2605a5d
work_keys_str_mv	AT ymorahernandez moleculartypingandantimicrobialresistanceprofilingof33mastitisrelatedstaphylococcusaureusisolatesfromcowsinthecomarcalaguneraregionofmexico AT everamurguia moleculartypingandantimicrobialresistanceprofilingof33mastitisrelatedstaphylococcusaureusisolatesfromcowsinthecomarcalaguneraregionofmexico AT jstinenbosch moleculartypingandantimicrobialresistanceprofilingof33mastitisrelatedstaphylococcusaureusisolatesfromcowsinthecomarcalaguneraregionofmexico AT phernandezjauregui moleculartypingandantimicrobialresistanceprofilingof33mastitisrelatedstaphylococcusaureusisolatesfromcowsinthecomarcalaguneraregionofmexico AT janmaartenvandijl moleculartypingandantimicrobialresistanceprofilingof33mastitisrelatedstaphylococcusaureusisolatesfromcowsinthecomarcalaguneraregionofmexico AT gbuist moleculartypingandantimicrobialresistanceprofilingof33mastitisrelatedstaphylococcusaureusisolatesfromcowsinthecomarcalaguneraregionofmexico
_version_	1718383651064905728

Molecular typing and antimicrobial resistance profiling of 33 mastitis-related Staphylococcus aureus isolates from cows in the Comarca Lagunera region of Mexico

Ejemplares similares