Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization

Abstract Oxygen (O2) concentration is approximately 5% in the fallopian tube and 2% in the uterus in humans. A “back to nature” approach could increase in vitro fertilization (IVF) outcomes. This hypothesis was tested in this monocentric observational retrospective study that included 120 couples wh...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sophie Brouillet, Chloé Baron, Fatima Barry, Aneta Andreeva, Delphine Haouzi, Anna Gala, Alice Ferrières-Hoa, Vanessa Loup, Tal Anahoryl, Noémie Ranisavljevic, Laura Gaspari, Samir Hamamah
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/acc30814cd1c49d48f2027ea033cb66e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:acc30814cd1c49d48f2027ea033cb66e
record_format	dspace
spelling	oai:doaj.org-article:acc30814cd1c49d48f2027ea033cb66e2021-11-21T12:23:25ZBiphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization10.1038/s41598-021-01782-62045-2322https://doaj.org/article/acc30814cd1c49d48f2027ea033cb66e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01782-6https://doaj.org/toc/2045-2322Abstract Oxygen (O2) concentration is approximately 5% in the fallopian tube and 2% in the uterus in humans. A “back to nature” approach could increase in vitro fertilization (IVF) outcomes. This hypothesis was tested in this monocentric observational retrospective study that included 120 couples who underwent two IVF cycles between 2014 and 2019. Embryos were cultured at 5% from day 0 (D0) to D5/6 (monophasic O2 concentration strategy) in the first IVF cycle, and at 5% O2 from D0 to D3 and 2% O2 from D3 to D5/6 (biphasic O2 concentration strategy) in the second IVF cycle. The total and usable blastocyst rates (44.4% vs. 54.8%, p = 0.049 and 21.8% vs. 32.8%, p = 0.002, respectively) and the cumulative live birth rate (17.9% vs. 44.1%, p = 0.027) were significantly higher with the biphasic (5%-2%) O2 concentration strategy. Whole transcriptome analysis of blastocysts donated for research identified 707 RNAs that were differentially expressed in function of the O2 strategy (fold-change > 2, p value < 0.05). These genes are mainly involved in embryo development, DNA repair, embryonic stem cell pluripotency, and implantation potential. The biphasic (5–2%) O2 concentration strategy for preimplantation embryo culture could increase the “take home baby rate”, thus improving IVF cost-effectiveness and infertility management.Sophie BrouilletChloé BaronFatima BarryAneta AndreevaDelphine HaouziAnna GalaAlice Ferrières-HoaVanessa LoupTal AnahorylNoémie RanisavljevicLaura GaspariSamir HamamahNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sophie Brouillet Chloé Baron Fatima Barry Aneta Andreeva Delphine Haouzi Anna Gala Alice Ferrières-Hoa Vanessa Loup Tal Anahoryl Noémie Ranisavljevic Laura Gaspari Samir Hamamah Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
description	Abstract Oxygen (O2) concentration is approximately 5% in the fallopian tube and 2% in the uterus in humans. A “back to nature” approach could increase in vitro fertilization (IVF) outcomes. This hypothesis was tested in this monocentric observational retrospective study that included 120 couples who underwent two IVF cycles between 2014 and 2019. Embryos were cultured at 5% from day 0 (D0) to D5/6 (monophasic O2 concentration strategy) in the first IVF cycle, and at 5% O2 from D0 to D3 and 2% O2 from D3 to D5/6 (biphasic O2 concentration strategy) in the second IVF cycle. The total and usable blastocyst rates (44.4% vs. 54.8%, p = 0.049 and 21.8% vs. 32.8%, p = 0.002, respectively) and the cumulative live birth rate (17.9% vs. 44.1%, p = 0.027) were significantly higher with the biphasic (5%-2%) O2 concentration strategy. Whole transcriptome analysis of blastocysts donated for research identified 707 RNAs that were differentially expressed in function of the O2 strategy (fold-change > 2, p value < 0.05). These genes are mainly involved in embryo development, DNA repair, embryonic stem cell pluripotency, and implantation potential. The biphasic (5–2%) O2 concentration strategy for preimplantation embryo culture could increase the “take home baby rate”, thus improving IVF cost-effectiveness and infertility management.
format	article
author	Sophie Brouillet Chloé Baron Fatima Barry Aneta Andreeva Delphine Haouzi Anna Gala Alice Ferrières-Hoa Vanessa Loup Tal Anahoryl Noémie Ranisavljevic Laura Gaspari Samir Hamamah
author_facet	Sophie Brouillet Chloé Baron Fatima Barry Aneta Andreeva Delphine Haouzi Anna Gala Alice Ferrières-Hoa Vanessa Loup Tal Anahoryl Noémie Ranisavljevic Laura Gaspari Samir Hamamah
author_sort	Sophie Brouillet
title	Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
title_short	Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
title_full	Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
title_fullStr	Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
title_full_unstemmed	Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
title_sort	biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/acc30814cd1c49d48f2027ea033cb66e
work_keys_str_mv	AT sophiebrouillet biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT chloebaron biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT fatimabarry biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT anetaandreeva biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT delphinehaouzi biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT annagala biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT aliceferriereshoa biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT vanessaloup biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT talanahoryl biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT noemieranisavljevic biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT lauragaspari biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization AT samirhamamah biphasic52oxygenconcentrationstrategysignificantlyimprovestheusableblastocystandcumulativelivebirthratesininvitrofertilization
_version_	1718419087049097216

Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization

Ejemplares similares