HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury

Junyang Chen,1 Xuefeng Li,1 Feng Zhao,2 Yubo Hu1 1Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Operating Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, Peopl...

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Autores principales:	Chen J, Li X, Zhao F, Hu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	ppf hotair mir-17-5p Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/acc5dda299af41bca3f1945e52c418da
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spelling	oai:doaj.org-article:acc5dda299af41bca3f1945e52c418da2021-12-02T14:39:09ZHOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury1178-1998https://doaj.org/article/acc5dda299af41bca3f1945e52c418da2021-04-01T00:00:00Zhttps://www.dovepress.com/hotairmir-17-5p-axis-is-involved-in-the-propofol-mediated-cardioprotec-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Junyang Chen,1 Xuefeng Li,1 Feng Zhao,2 Yubo Hu1 1Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Operating Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of ChinaCorrespondence: Yubo HuDepartment of Anesthesiology, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin 130033, People’s Republic of ChinaEmail huyubo717@jlu.edu.cnBackground: Propofol (PPF) ameliorates ischemia/reperfusion (I/R) injury in multiple organs by reducing apoptosis and release of pro-inflammatory cytokines. This study aims to explore the mechanism of PPF in attenuating myocardial ischemia-reperfusion injury (MIRI).Materials and Methods: Rat MIRI model was established, and PPF pre-treatment was performed 10 min before I/R. H9c2 cardiomyocytes treated with hypoxia/reoxygenation (H/R) were used to establish an in vitro model. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to evaluate HOTAIR and miR-17-5p expression levels. Flow cytometry was employed to detect the apoptosis of H9c2 cells. The interaction between HOTAIR and miR-17-5p was determined by bioinformatics analysis, luciferase reporter gene analysis, and RNA immunoprecipitation experiments. STAT3 and p-STAT3 expressions were detected by Western blot.Results: PPF pre-treatment significantly reduced creatine kinase isoenzyme (CK-MB) and serum lactate dehydrogenase (LDH) levels in the serum of the rats with MIRI. PPF pre-treatment remarkably up-regulated HOTAIR expression and down-regulated miR-17-5p expression in both in vivo and in vitro models. HOTAIR adsorbed miR-17-5p to repress the expression of miR-17-5p. PPF pre-treatment markedly inhibited cardiomyocyte apoptosis induced by I/R or H/R. HOTAIR knockdown could partially reverse the protective effects of PPF on MIRI. HOTAIR could activate STAT3 signaling via repressing miR-17-5p expression.Conclusion: PPF protects the MIRI by modulating the HOTAIR/miR-17-5p/STAT3 axis.Keywords: PPF, HOTAIR, miR-17-5pChen JLi XZhao FHu YDove Medical Pressarticleppfhotairmir-17-5pGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 16, Pp 621-632 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ppf hotair mir-17-5p Geriatrics RC952-954.6
spellingShingle	ppf hotair mir-17-5p Geriatrics RC952-954.6 Chen J Li X Zhao F Hu Y HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
description	Junyang Chen,1 Xuefeng Li,1 Feng Zhao,2 Yubo Hu1 1Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Operating Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of ChinaCorrespondence: Yubo HuDepartment of Anesthesiology, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin 130033, People’s Republic of ChinaEmail huyubo717@jlu.edu.cnBackground: Propofol (PPF) ameliorates ischemia/reperfusion (I/R) injury in multiple organs by reducing apoptosis and release of pro-inflammatory cytokines. This study aims to explore the mechanism of PPF in attenuating myocardial ischemia-reperfusion injury (MIRI).Materials and Methods: Rat MIRI model was established, and PPF pre-treatment was performed 10 min before I/R. H9c2 cardiomyocytes treated with hypoxia/reoxygenation (H/R) were used to establish an in vitro model. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to evaluate HOTAIR and miR-17-5p expression levels. Flow cytometry was employed to detect the apoptosis of H9c2 cells. The interaction between HOTAIR and miR-17-5p was determined by bioinformatics analysis, luciferase reporter gene analysis, and RNA immunoprecipitation experiments. STAT3 and p-STAT3 expressions were detected by Western blot.Results: PPF pre-treatment significantly reduced creatine kinase isoenzyme (CK-MB) and serum lactate dehydrogenase (LDH) levels in the serum of the rats with MIRI. PPF pre-treatment remarkably up-regulated HOTAIR expression and down-regulated miR-17-5p expression in both in vivo and in vitro models. HOTAIR adsorbed miR-17-5p to repress the expression of miR-17-5p. PPF pre-treatment markedly inhibited cardiomyocyte apoptosis induced by I/R or H/R. HOTAIR knockdown could partially reverse the protective effects of PPF on MIRI. HOTAIR could activate STAT3 signaling via repressing miR-17-5p expression.Conclusion: PPF protects the MIRI by modulating the HOTAIR/miR-17-5p/STAT3 axis.Keywords: PPF, HOTAIR, miR-17-5p
format	article
author	Chen J Li X Zhao F Hu Y
author_facet	Chen J Li X Zhao F Hu Y
author_sort	Chen J
title	HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
title_short	HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
title_full	HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
title_fullStr	HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
title_full_unstemmed	HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
title_sort	hotair/mir-17-5p axis is involved in the propofol-mediated cardioprotection against ischemia/reperfusion injury
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/acc5dda299af41bca3f1945e52c418da
work_keys_str_mv	AT chenj hotairmir175paxisisinvolvedinthepropofolmediatedcardioprotectionagainstischemiareperfusioninjury AT lix hotairmir175paxisisinvolvedinthepropofolmediatedcardioprotectionagainstischemiareperfusioninjury AT zhaof hotairmir175paxisisinvolvedinthepropofolmediatedcardioprotectionagainstischemiareperfusioninjury AT huy hotairmir175paxisisinvolvedinthepropofolmediatedcardioprotectionagainstischemiareperfusioninjury
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HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury

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