Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.

Placental malaria is a common clinical complication during pregnancy and is associated with abortion, premature delivery, intrauterine growth retardation and low birth weight. The present study was designed to delineate the underlying mechanism of placental pathology during malarial infection with s...

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Autores principales:	Lalita Sharma, Jagdeep Kaur, Geeta Shukla
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/acc6e7ec2aab4f4fa4b0cb27cc2a90af
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spelling	oai:doaj.org-article:acc6e7ec2aab4f4fa4b0cb27cc2a90af2021-11-18T07:26:16ZRole of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.1932-620310.1371/journal.pone.0032694https://doaj.org/article/acc6e7ec2aab4f4fa4b0cb27cc2a90af2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22396790/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Placental malaria is a common clinical complication during pregnancy and is associated with abortion, premature delivery, intrauterine growth retardation and low birth weight. The present study was designed to delineate the underlying mechanism of placental pathology during malarial infection with special reference to oxidative stress and apoptosis. Experimentally, pregnant BALB/c mice were infected with Plasmodium berghei infected red blood cells on gestation day 10. The presence of malarial infection in placenta was confirmed by histopathological studies. It was observation that infected placenta had plugged placental sinusoids with parasitized red blood cells and malarial pigments. Interestingly, we found significant increase in the level of malondialdehyde, the index of oxidative stress and decreased activity of catalase, the antioxidant in infected placenta. Furthermore, in infected placenta the oxidative stress mediated apoptosis was determined by DNA fragmentation assay, ethidium bromide/acridine orange staining and caspase activity. It was observed that oxidative stress begin after second day of malarial infection. Interestingly, it was observed that there was down regulation of anti-apoptotic protein Bcl-2 and up regulation of pro-apoptotic protein Bax in infected placenta, suggesting the involvement of mitochondrial pathway of apoptosis which was further confirmed by activation of caspase 9. However, no change in the expression of Fas gene and caspase 8 activity, indicated the absence of death receptor pathway. Thus, it can be concluded that the placental pathology during malarial infection is mediated by mitochondrial pathway of apoptosis occurring due to augmented lipid peroxidation which may in turn jeopardise the materno-fetal relationship.Lalita SharmaJagdeep KaurGeeta ShuklaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32694 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lalita Sharma Jagdeep Kaur Geeta Shukla Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.
description	Placental malaria is a common clinical complication during pregnancy and is associated with abortion, premature delivery, intrauterine growth retardation and low birth weight. The present study was designed to delineate the underlying mechanism of placental pathology during malarial infection with special reference to oxidative stress and apoptosis. Experimentally, pregnant BALB/c mice were infected with Plasmodium berghei infected red blood cells on gestation day 10. The presence of malarial infection in placenta was confirmed by histopathological studies. It was observation that infected placenta had plugged placental sinusoids with parasitized red blood cells and malarial pigments. Interestingly, we found significant increase in the level of malondialdehyde, the index of oxidative stress and decreased activity of catalase, the antioxidant in infected placenta. Furthermore, in infected placenta the oxidative stress mediated apoptosis was determined by DNA fragmentation assay, ethidium bromide/acridine orange staining and caspase activity. It was observed that oxidative stress begin after second day of malarial infection. Interestingly, it was observed that there was down regulation of anti-apoptotic protein Bcl-2 and up regulation of pro-apoptotic protein Bax in infected placenta, suggesting the involvement of mitochondrial pathway of apoptosis which was further confirmed by activation of caspase 9. However, no change in the expression of Fas gene and caspase 8 activity, indicated the absence of death receptor pathway. Thus, it can be concluded that the placental pathology during malarial infection is mediated by mitochondrial pathway of apoptosis occurring due to augmented lipid peroxidation which may in turn jeopardise the materno-fetal relationship.
format	article
author	Lalita Sharma Jagdeep Kaur Geeta Shukla
author_facet	Lalita Sharma Jagdeep Kaur Geeta Shukla
author_sort	Lalita Sharma
title	Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.
title_short	Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.
title_full	Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.
title_fullStr	Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.
title_full_unstemmed	Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.
title_sort	role of oxidative stress and apoptosis in the placental pathology of plasmodium berghei infected mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/acc6e7ec2aab4f4fa4b0cb27cc2a90af
work_keys_str_mv	AT lalitasharma roleofoxidativestressandapoptosisintheplacentalpathologyofplasmodiumbergheiinfectedmice AT jagdeepkaur roleofoxidativestressandapoptosisintheplacentalpathologyofplasmodiumbergheiinfectedmice AT geetashukla roleofoxidativestressandapoptosisintheplacentalpathologyofplasmodiumbergheiinfectedmice
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Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice.

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