Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity

Summary Next to d‐glucose, the pentoses l‐arabinose and d‐xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best‐studied pathw...

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Autores principales:	Tania Chroumpi, Mao Peng, Maria Victoria Aguilar‐Pontes, Astrid Müller, Mei Wang, Juying Yan, Anna Lipzen, Vivian Ng, Igor V. Grigoriev, Miia R. Mäkelä, Ronald P. deVries
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/acc8dbe60744405db693deec80e04222
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spelling	oai:doaj.org-article:acc8dbe60744405db693deec80e042222021-11-18T15:39:53ZRevisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity1751-791510.1111/1751-7915.13790https://doaj.org/article/acc8dbe60744405db693deec80e042222021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13790https://doaj.org/toc/1751-7915Summary Next to d‐glucose, the pentoses l‐arabinose and d‐xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best‐studied pathways of primary metabolism of Aspergillus niger, and an initial outline of this pathway with individual enzymes covering each step of the pathway has been previously established. However, although growth on l‐arabinose and/or d‐xylose of most pentose catabolic pathway (PCP) single deletion mutants of A. niger has been shown to be negatively affected, it was not abolished, suggesting the involvement of additional enzymes. Detailed analysis of the single deletion mutants of the known A. niger PCP genes led to the identification of additional genes involved in the pathway. These results reveal a high level of complexity and redundancy in this pathway, emphasizing the need for a comprehensive understanding of metabolic pathways before entering metabolic engineering of such pathways for the generation of more efficient fungal cell factories.Tania ChroumpiMao PengMaria Victoria Aguilar‐PontesAstrid MüllerMei WangJuying YanAnna LipzenVivian NgIgor V. GrigorievMiia R. MäkeläRonald P. deVriesWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2525-2537 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Tania Chroumpi Mao Peng Maria Victoria Aguilar‐Pontes Astrid Müller Mei Wang Juying Yan Anna Lipzen Vivian Ng Igor V. Grigoriev Miia R. Mäkelä Ronald P. deVries Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
description	Summary Next to d‐glucose, the pentoses l‐arabinose and d‐xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best‐studied pathways of primary metabolism of Aspergillus niger, and an initial outline of this pathway with individual enzymes covering each step of the pathway has been previously established. However, although growth on l‐arabinose and/or d‐xylose of most pentose catabolic pathway (PCP) single deletion mutants of A. niger has been shown to be negatively affected, it was not abolished, suggesting the involvement of additional enzymes. Detailed analysis of the single deletion mutants of the known A. niger PCP genes led to the identification of additional genes involved in the pathway. These results reveal a high level of complexity and redundancy in this pathway, emphasizing the need for a comprehensive understanding of metabolic pathways before entering metabolic engineering of such pathways for the generation of more efficient fungal cell factories.
format	article
author	Tania Chroumpi Mao Peng Maria Victoria Aguilar‐Pontes Astrid Müller Mei Wang Juying Yan Anna Lipzen Vivian Ng Igor V. Grigoriev Miia R. Mäkelä Ronald P. deVries
author_facet	Tania Chroumpi Mao Peng Maria Victoria Aguilar‐Pontes Astrid Müller Mei Wang Juying Yan Anna Lipzen Vivian Ng Igor V. Grigoriev Miia R. Mäkelä Ronald P. deVries
author_sort	Tania Chroumpi
title	Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
title_short	Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
title_full	Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
title_fullStr	Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
title_full_unstemmed	Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
title_sort	revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/acc8dbe60744405db693deec80e04222
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Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity

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