Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer

Circular RNAs (circRNAs), which form as covalently closed loop structures, have several biological functions such as regulation of cellular behavior by adsorbing microRNAs. However, there is limited information of circRNAs in oral squamous cell carcinoma (OSCC). Here, we aimed to elucidate the roles...

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Autores principales:	Toshiaki Ando, Atsushi Kasamatsu, Kohei Kawasaki, Kazuya Hiroshima, Reo Fukushima, Manabu Iyoda, Dai Nakashima, Yosuke Endo-Sakamoto, Katsuhiro Uzawa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	circRNA miRNA droplet digital PCR liquid biopsy regional lymph node metastasis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/accdee98190149d4b78f9465ff49c9e5
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spelling	oai:doaj.org-article:accdee98190149d4b78f9465ff49c9e52021-11-25T17:02:56ZTumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer10.3390/cancers132257082072-6694https://doaj.org/article/accdee98190149d4b78f9465ff49c9e52021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5708https://doaj.org/toc/2072-6694Circular RNAs (circRNAs), which form as covalently closed loop structures, have several biological functions such as regulation of cellular behavior by adsorbing microRNAs. However, there is limited information of circRNAs in oral squamous cell carcinoma (OSCC). Here, we aimed to elucidate the roles of aberrantly expressed circRNAs in OSCC. CircRNA microarray showed that circRNA-102450 was down-regulated in OSCC cells. Clinical validation of circRNA-102450 was performed using highly sensitive droplet digital PCR in preoperative liquid biopsy samples from 30 OSCC patients. Interestingly, none of 16 studied patients with high circRNA-102450 had regional lymph node metastasis (RLNM), whereas 4 of 14 studied patients (28.5%) with low expression had pathologically proven RLNM. Overexpressed circRNA-102450 significantly inhibited the tumor metastatic properties of cell proliferation, migration, and invasion. Furthermore, circRNA-102450 directly bound to, and consequently down-regulated, miR-1178 in OSCC cells. Taken together, circRNA-102450 has a tumor suppressive effect via the circRNA-102450/miR-1178 axis and may be a novel potential marker of RLNM in OSCC patients.Toshiaki AndoAtsushi KasamatsuKohei KawasakiKazuya HiroshimaReo FukushimaManabu IyodaDai NakashimaYosuke Endo-SakamotoKatsuhiro UzawaMDPI AGarticlecircRNAmiRNAdroplet digital PCRliquid biopsyregional lymph node metastasisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5708, p 5708 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circRNA miRNA droplet digital PCR liquid biopsy regional lymph node metastasis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	circRNA miRNA droplet digital PCR liquid biopsy regional lymph node metastasis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Toshiaki Ando Atsushi Kasamatsu Kohei Kawasaki Kazuya Hiroshima Reo Fukushima Manabu Iyoda Dai Nakashima Yosuke Endo-Sakamoto Katsuhiro Uzawa Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer
description	Circular RNAs (circRNAs), which form as covalently closed loop structures, have several biological functions such as regulation of cellular behavior by adsorbing microRNAs. However, there is limited information of circRNAs in oral squamous cell carcinoma (OSCC). Here, we aimed to elucidate the roles of aberrantly expressed circRNAs in OSCC. CircRNA microarray showed that circRNA-102450 was down-regulated in OSCC cells. Clinical validation of circRNA-102450 was performed using highly sensitive droplet digital PCR in preoperative liquid biopsy samples from 30 OSCC patients. Interestingly, none of 16 studied patients with high circRNA-102450 had regional lymph node metastasis (RLNM), whereas 4 of 14 studied patients (28.5%) with low expression had pathologically proven RLNM. Overexpressed circRNA-102450 significantly inhibited the tumor metastatic properties of cell proliferation, migration, and invasion. Furthermore, circRNA-102450 directly bound to, and consequently down-regulated, miR-1178 in OSCC cells. Taken together, circRNA-102450 has a tumor suppressive effect via the circRNA-102450/miR-1178 axis and may be a novel potential marker of RLNM in OSCC patients.
format	article
author	Toshiaki Ando Atsushi Kasamatsu Kohei Kawasaki Kazuya Hiroshima Reo Fukushima Manabu Iyoda Dai Nakashima Yosuke Endo-Sakamoto Katsuhiro Uzawa
author_facet	Toshiaki Ando Atsushi Kasamatsu Kohei Kawasaki Kazuya Hiroshima Reo Fukushima Manabu Iyoda Dai Nakashima Yosuke Endo-Sakamoto Katsuhiro Uzawa
author_sort	Toshiaki Ando
title	Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer
title_short	Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer
title_full	Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer
title_fullStr	Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer
title_full_unstemmed	Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer
title_sort	tumor suppressive circular rna-102450: development of a novel diagnostic procedure for lymph node metastasis from oral cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/accdee98190149d4b78f9465ff49c9e5
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Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer

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