Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration

The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to b...

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Autores principales: Elia Bosch-Rué, Leire Díez-Tercero, Raquel Rodríguez-González, Begoña María Bosch-Canals, Roman A. Perez
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Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/acce5dfa44834c86b554914382c6c634
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spelling oai:doaj.org-article:acce5dfa44834c86b554914382c6c6342021-11-04T06:49:40ZAssessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration1932-6203https://doaj.org/article/acce5dfa44834c86b554914382c6c6342021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550415/?tool=EBIhttps://doaj.org/toc/1932-6203The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation.Elia Bosch-RuéLeire Díez-TerceroRaquel Rodríguez-GonzálezBegoña María Bosch-CanalsRoman A. PerezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Elia Bosch-Rué
Leire Díez-Tercero
Raquel Rodríguez-González
Begoña María Bosch-Canals
Roman A. Perez
Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
description The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation.
format article
author Elia Bosch-Rué
Leire Díez-Tercero
Raquel Rodríguez-González
Begoña María Bosch-Canals
Roman A. Perez
author_facet Elia Bosch-Rué
Leire Díez-Tercero
Raquel Rodríguez-González
Begoña María Bosch-Canals
Roman A. Perez
author_sort Elia Bosch-Rué
title Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
title_short Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
title_full Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
title_fullStr Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
title_full_unstemmed Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
title_sort assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/acce5dfa44834c86b554914382c6c634
work_keys_str_mv AT eliaboschrue assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration
AT leiredieztercero assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration
AT raquelrodriguezgonzalez assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration
AT begonamariaboschcanals assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration
AT romanaperez assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration
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