Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia

Introduction: In this study, we determined the influence of intrinsic exercise capacity on the vascular adaptive responses to hind limb ischemia. High Capacity Running, HCR; Low Capacity Running, LCR, rats were used to assess intrinsic aerobic capacity effects on adaptive responses to ischemia.Metho...

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Autores principales:	Elizabeth Granier, Madaniah O. Zakari, Musaad B. Alsahly, Lauren G. Koch, Steven Britton, Laxmansa C. Katwa, Robert M. Lust
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	vascular occlusion aerobic running capacity exercise peripheral artery occlusion limb ischemia Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/accf8cde6b2b486aaa07fce44942de0d
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spelling	oai:doaj.org-article:accf8cde6b2b486aaa07fce44942de0d2021-11-22T05:00:26ZLow Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia2297-055X10.3389/fcvm.2021.752955https://doaj.org/article/accf8cde6b2b486aaa07fce44942de0d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.752955/fullhttps://doaj.org/toc/2297-055XIntroduction: In this study, we determined the influence of intrinsic exercise capacity on the vascular adaptive responses to hind limb ischemia. High Capacity Running, HCR; Low Capacity Running, LCR, rats were used to assess intrinsic aerobic capacity effects on adaptive responses to ischemia.Methods: Muscle samples from both ischemic and non-ischemic limb in both strains were compared, histologically for the muscle-capillary relationship, and functionally using microspheres to track blood flow and muscle stimulation to test fatigability. PCR was used to identify the differences in gene expression between the phenotypes following occlusive ischemia.Results: Prior to ligation, there were not significant differences between the phenotypes in the exhaustion time with high frequency pacing. Following ligation, LCR decreased significantly in the exhaustion time compare with HCRs (437 ± 47 vs. 824 ± 56, p < 0.001). The immediate decrease in flow was significantly more severe in LCRs than HCRs (52.5 vs. 37.8%, p < 0.001). VEGF, eNOS, and ANG2 (but not ANG1) gene expression were decreased in LCRs vs. HCRs before occlusion, and increased significantly in LCRs 14D after occlusion, but not in HCRs. LCR capillary density (CD) was significantly lower at all time points after occlusion (LCR 7D = 564.76 ± 40.5, LCR 14D = 507.48 ± 54.2, both p < 0.05 vs. HCR for respective time point). NCAF increased significantly in HCR and LCR in response to ischemia.Summary: These results suggest that LCR confers increased risk for ischemic injury and is subject to delayed and less effective adaptive response to ischemic stress.Elizabeth GranierElizabeth GranierMadaniah O. ZakariMadaniah O. ZakariMusaad B. AlsahlyMusaad B. AlsahlyLauren G. KochSteven BrittonLaxmansa C. KatwaRobert M. LustRobert M. LustFrontiers Media S.A.articlevascular occlusionaerobic running capacityexerciseperipheral artery occlusionlimb ischemiaDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vascular occlusion aerobic running capacity exercise peripheral artery occlusion limb ischemia Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	vascular occlusion aerobic running capacity exercise peripheral artery occlusion limb ischemia Diseases of the circulatory (Cardiovascular) system RC666-701 Elizabeth Granier Elizabeth Granier Madaniah O. Zakari Madaniah O. Zakari Musaad B. Alsahly Musaad B. Alsahly Lauren G. Koch Steven Britton Laxmansa C. Katwa Robert M. Lust Robert M. Lust Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
description	Introduction: In this study, we determined the influence of intrinsic exercise capacity on the vascular adaptive responses to hind limb ischemia. High Capacity Running, HCR; Low Capacity Running, LCR, rats were used to assess intrinsic aerobic capacity effects on adaptive responses to ischemia.Methods: Muscle samples from both ischemic and non-ischemic limb in both strains were compared, histologically for the muscle-capillary relationship, and functionally using microspheres to track blood flow and muscle stimulation to test fatigability. PCR was used to identify the differences in gene expression between the phenotypes following occlusive ischemia.Results: Prior to ligation, there were not significant differences between the phenotypes in the exhaustion time with high frequency pacing. Following ligation, LCR decreased significantly in the exhaustion time compare with HCRs (437 ± 47 vs. 824 ± 56, p < 0.001). The immediate decrease in flow was significantly more severe in LCRs than HCRs (52.5 vs. 37.8%, p < 0.001). VEGF, eNOS, and ANG2 (but not ANG1) gene expression were decreased in LCRs vs. HCRs before occlusion, and increased significantly in LCRs 14D after occlusion, but not in HCRs. LCR capillary density (CD) was significantly lower at all time points after occlusion (LCR 7D = 564.76 ± 40.5, LCR 14D = 507.48 ± 54.2, both p < 0.05 vs. HCR for respective time point). NCAF increased significantly in HCR and LCR in response to ischemia.Summary: These results suggest that LCR confers increased risk for ischemic injury and is subject to delayed and less effective adaptive response to ischemic stress.
format	article
author	Elizabeth Granier Elizabeth Granier Madaniah O. Zakari Madaniah O. Zakari Musaad B. Alsahly Musaad B. Alsahly Lauren G. Koch Steven Britton Laxmansa C. Katwa Robert M. Lust Robert M. Lust
author_facet	Elizabeth Granier Elizabeth Granier Madaniah O. Zakari Madaniah O. Zakari Musaad B. Alsahly Musaad B. Alsahly Lauren G. Koch Steven Britton Laxmansa C. Katwa Robert M. Lust Robert M. Lust
author_sort	Elizabeth Granier
title	Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
title_short	Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
title_full	Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
title_fullStr	Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
title_full_unstemmed	Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
title_sort	low intrinsic aerobic capacity limits recovery response to hindlimb ischemia
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/accf8cde6b2b486aaa07fce44942de0d
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_version_	1718418179879862272

Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia

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