Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Yung-Hen Hsu,1,2 Chang-Tun Lin,2 Yi-Hsun Yu,1,2 Ying-Chao Chou,1,2 Shih-Jung Liu,1,2 Err-Cheng Chan3 1Department of Orthopedic Surgery, Chang Gung Memorial Hospital, 2Department of Mechanical Engineering, 3Department of Medical Biotechnology and Laboratory Science, School of Medical Technology, Chan...

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Autores principales:	Hsu YH, Lin CT, Yu YH, Chou YC, Liu SJ, Chan EC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	biodegradable sheath-core nanofibers coaxial electrospinning vancomycin ceftazidime rhBMP-2 release characteristics Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/acdd3aec09c34e57bdc993731b76f773
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spelling	oai:doaj.org-article:acdd3aec09c34e57bdc993731b76f7732021-12-02T05:40:29ZDual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers1178-2013https://doaj.org/article/acdd3aec09c34e57bdc993731b76f7732016-08-01T00:00:00Zhttps://www.dovepress.com/dual-delivery-of-active-antibactericidal-agents-and-bone-morphogenetic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yung-Hen Hsu,1,2 Chang-Tun Lin,2 Yi-Hsun Yu,1,2 Ying-Chao Chou,1,2 Shih-Jung Liu,1,2 Err-Cheng Chan3 1Department of Orthopedic Surgery, Chang Gung Memorial Hospital, 2Department of Mechanical Engineering, 3Department of Medical Biotechnology and Laboratory Science, School of Medical Technology, Chang Gung University, Tao-Yuan, Taiwan Abstract: In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly(D,L)-lactide-co-glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly(D,L)-lactide-co-glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC]90) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries. Keywords: biodegradable sheath-core nanofibers, coaxial electrospinning, vancomycin, ceftazidime, rhBMP-2, release characteristicsHsu YHLin CTYu YHChou YCLiu SJChan ECDove Medical Pressarticlebiodegradable sheath-core nanofiberscoaxial electrospinningvancomycinceftazidimerhBMP-2release characteristicsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 3927-3937 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodegradable sheath-core nanofibers coaxial electrospinning vancomycin ceftazidime rhBMP-2 release characteristics Medicine (General) R5-920
spellingShingle	biodegradable sheath-core nanofibers coaxial electrospinning vancomycin ceftazidime rhBMP-2 release characteristics Medicine (General) R5-920 Hsu YH Lin CT Yu YH Chou YC Liu SJ Chan EC Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
description	Yung-Hen Hsu,1,2 Chang-Tun Lin,2 Yi-Hsun Yu,1,2 Ying-Chao Chou,1,2 Shih-Jung Liu,1,2 Err-Cheng Chan3 1Department of Orthopedic Surgery, Chang Gung Memorial Hospital, 2Department of Mechanical Engineering, 3Department of Medical Biotechnology and Laboratory Science, School of Medical Technology, Chang Gung University, Tao-Yuan, Taiwan Abstract: In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly(D,L)-lactide-co-glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly(D,L)-lactide-co-glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC]90) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries. Keywords: biodegradable sheath-core nanofibers, coaxial electrospinning, vancomycin, ceftazidime, rhBMP-2, release characteristics
format	article
author	Hsu YH Lin CT Yu YH Chou YC Liu SJ Chan EC
author_facet	Hsu YH Lin CT Yu YH Chou YC Liu SJ Chan EC
author_sort	Hsu YH
title	Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
title_short	Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
title_full	Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
title_fullStr	Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
title_full_unstemmed	Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
title_sort	dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/acdd3aec09c34e57bdc993731b76f773
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Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

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