Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer

Tumor associated neutrophils (TANs) and cytotoxic T cells (CTLs) are part of the tumor microenvironment of gastric cancer (GC). We explored their tumor biological significance in neoadjuvantly/perioperatively treated GC. Immunostaining was performed on whole tissue sections of 173 GCs, using antibod...

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Autores principales:	Anne Hoffmann, Hans-Michael Behrens, Steffen Heckl, Sandra Krüger, Thomas Becker, Christoph Röcken
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gastric cancer tumor associated neutrophils cytotoxic T cells Medicine R
Acceso en línea:	https://doaj.org/article/ace179e4e8d84356a43868d695c60200
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spelling	oai:doaj.org-article:ace179e4e8d84356a43868d695c602002021-11-25T18:07:52ZNeoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer10.3390/jpm111111842075-4426https://doaj.org/article/ace179e4e8d84356a43868d695c602002021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1184https://doaj.org/toc/2075-4426Tumor associated neutrophils (TANs) and cytotoxic T cells (CTLs) are part of the tumor microenvironment of gastric cancer (GC). We explored their tumor biological significance in neoadjuvantly/perioperatively treated GC. Immunostaining was performed on whole tissue sections of 173 GCs, using antibodies directed against myeloperoxidase (MPO) and CD8. Stained specimens were digitalized, and the densities of TANs and CTLs were assessed separately in the mucosa, tumor surface, tumor center, invasion front, and tumor scar. The densities were correlated with clinicopathological patient characteristics. Compared with a historical cohort of 449 treatment naive GCs, the TAN density in the invasion front was significantly lower in neoadjuvantly/perioperatively treated GCs. TAN density in the tumor center and invasion front correlated with tumor regression. TAN density also correlated with CTL density in the tumor center and invasion front. A high density of CTL in the tumor center correlated with an improved overall survival and tumor specific survival. We show that neoadjuvant/perioperative (radio-) chemotherapy impacts on the immune microenvironment of GC, while also depending on sex. The density of TANs in neoadjuvantly/perioperatively treated GCs differed from findings made in a treatment naive GC cohort.Anne HoffmannHans-Michael BehrensSteffen HecklSandra KrügerThomas BeckerChristoph RöckenMDPI AGarticlegastric cancertumor associated neutrophilscytotoxic T cellsMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1184, p 1184 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastric cancer tumor associated neutrophils cytotoxic T cells Medicine R
spellingShingle	gastric cancer tumor associated neutrophils cytotoxic T cells Medicine R Anne Hoffmann Hans-Michael Behrens Steffen Heckl Sandra Krüger Thomas Becker Christoph Röcken Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
description	Tumor associated neutrophils (TANs) and cytotoxic T cells (CTLs) are part of the tumor microenvironment of gastric cancer (GC). We explored their tumor biological significance in neoadjuvantly/perioperatively treated GC. Immunostaining was performed on whole tissue sections of 173 GCs, using antibodies directed against myeloperoxidase (MPO) and CD8. Stained specimens were digitalized, and the densities of TANs and CTLs were assessed separately in the mucosa, tumor surface, tumor center, invasion front, and tumor scar. The densities were correlated with clinicopathological patient characteristics. Compared with a historical cohort of 449 treatment naive GCs, the TAN density in the invasion front was significantly lower in neoadjuvantly/perioperatively treated GCs. TAN density in the tumor center and invasion front correlated with tumor regression. TAN density also correlated with CTL density in the tumor center and invasion front. A high density of CTL in the tumor center correlated with an improved overall survival and tumor specific survival. We show that neoadjuvant/perioperative (radio-) chemotherapy impacts on the immune microenvironment of GC, while also depending on sex. The density of TANs in neoadjuvantly/perioperatively treated GCs differed from findings made in a treatment naive GC cohort.
format	article
author	Anne Hoffmann Hans-Michael Behrens Steffen Heckl Sandra Krüger Thomas Becker Christoph Röcken
author_facet	Anne Hoffmann Hans-Michael Behrens Steffen Heckl Sandra Krüger Thomas Becker Christoph Röcken
author_sort	Anne Hoffmann
title	Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
title_short	Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
title_full	Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
title_fullStr	Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
title_full_unstemmed	Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
title_sort	neoadjuvant/perioperative treatment affects spatial distribution and densities of tumor associated neutrophils and cd8+ lymphocytes in gastric cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ace179e4e8d84356a43868d695c60200
work_keys_str_mv	AT annehoffmann neoadjuvantperioperativetreatmentaffectsspatialdistributionanddensitiesoftumorassociatedneutrophilsandcd8lymphocytesingastriccancer AT hansmichaelbehrens neoadjuvantperioperativetreatmentaffectsspatialdistributionanddensitiesoftumorassociatedneutrophilsandcd8lymphocytesingastriccancer AT steffenheckl neoadjuvantperioperativetreatmentaffectsspatialdistributionanddensitiesoftumorassociatedneutrophilsandcd8lymphocytesingastriccancer AT sandrakruger neoadjuvantperioperativetreatmentaffectsspatialdistributionanddensitiesoftumorassociatedneutrophilsandcd8lymphocytesingastriccancer AT thomasbecker neoadjuvantperioperativetreatmentaffectsspatialdistributionanddensitiesoftumorassociatedneutrophilsandcd8lymphocytesingastriccancer AT christophrocken neoadjuvantperioperativetreatmentaffectsspatialdistributionanddensitiesoftumorassociatedneutrophilsandcd8lymphocytesingastriccancer
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Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer

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