Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand

Abstract Nontyphoidal Salmonella, an important zoonotic pathogen and a major cause of foodborne illnesses, could be a potential reservoir of plasmids harbouring mobile colistin resistance gene (mcr). This study reported, for the first time, a high rate of mcr-carrying Salmonella clinical isolates (3...

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Autores principales:	Sirirat Luk-in, Tanittha Chatsuwan, Naris Kueakulpattana, Ubolrat Rirerm, Dhammika Leshan Wannigama, Rongpong Plongla, Ratana Lawung, Chaiwat Pulsrikarn, Siriporn Chantaroj, Pattharaporn Chaichana, Nattida Saksaengsopa, Teerarat Shanthachol, Malee Techapornroong, Sunee Chayangsu, Wanla Kulwichit
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ace9521af3ae4eb08001957d43bbe40e
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spelling	oai:doaj.org-article:ace9521af3ae4eb08001957d43bbe40e2021-12-02T16:14:47ZOccurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand10.1038/s41598-021-93529-62045-2322https://doaj.org/article/ace9521af3ae4eb08001957d43bbe40e2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93529-6https://doaj.org/toc/2045-2322Abstract Nontyphoidal Salmonella, an important zoonotic pathogen and a major cause of foodborne illnesses, could be a potential reservoir of plasmids harbouring mobile colistin resistance gene (mcr). This study reported, for the first time, a high rate of mcr-carrying Salmonella clinical isolates (3.3%, 24/724) in Thailand, associated with mcr-3 gene (3.0%, 22/724) in S. 4,[5],12:i:-(15.4%, 4/26), S. Typhimurium (8.8%, 5/57), and S. Choleraesuis (5.6%, 13/231). Remarkably, the increasing trends of colistin and extended-spectrum cephalosporin resistances have displayed a high agreement over the years, with a dramatic rise in the mcr-carrying Salmonella from 1.1% (6/563) during 2005–2007 to 11.2% (18/161) during 2014–2018 when CTX-M-55 became abundant. Clonal and plasmid analysis revealed that the self-transferable IncA/C and a novel hybrid IncA/C-FIIs MDR plasmids were the major vehicles to disseminate both mcr-3 and bla CTX-M55 genes among diverse Salmonella strains, from as early as 2007. To our knowledge the occurrence of mcr-3 and the co-existence of it with bla CTX-M-55 in S. Choleraesuis are reported here for the first time, leading to clinical concern over the treatment of the invasive salmonellosis. This study provides evidence of the potential reservoirs and vectors in the dissemination of the mcr and highlights the co-selection by colistin and/or cephalosporins.Sirirat Luk-inTanittha ChatsuwanNaris KueakulpattanaUbolrat RirermDhammika Leshan WannigamaRongpong PlonglaRatana LawungChaiwat PulsrikarnSiriporn ChantarojPattharaporn ChaichanaNattida SaksaengsopaTeerarat ShanthacholMalee TechapornroongSunee ChayangsuWanla KulwichitNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sirirat Luk-in Tanittha Chatsuwan Naris Kueakulpattana Ubolrat Rirerm Dhammika Leshan Wannigama Rongpong Plongla Ratana Lawung Chaiwat Pulsrikarn Siriporn Chantaroj Pattharaporn Chaichana Nattida Saksaengsopa Teerarat Shanthachol Malee Techapornroong Sunee Chayangsu Wanla Kulwichit Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand
description	Abstract Nontyphoidal Salmonella, an important zoonotic pathogen and a major cause of foodborne illnesses, could be a potential reservoir of plasmids harbouring mobile colistin resistance gene (mcr). This study reported, for the first time, a high rate of mcr-carrying Salmonella clinical isolates (3.3%, 24/724) in Thailand, associated with mcr-3 gene (3.0%, 22/724) in S. 4,[5],12:i:-(15.4%, 4/26), S. Typhimurium (8.8%, 5/57), and S. Choleraesuis (5.6%, 13/231). Remarkably, the increasing trends of colistin and extended-spectrum cephalosporin resistances have displayed a high agreement over the years, with a dramatic rise in the mcr-carrying Salmonella from 1.1% (6/563) during 2005–2007 to 11.2% (18/161) during 2014–2018 when CTX-M-55 became abundant. Clonal and plasmid analysis revealed that the self-transferable IncA/C and a novel hybrid IncA/C-FIIs MDR plasmids were the major vehicles to disseminate both mcr-3 and bla CTX-M55 genes among diverse Salmonella strains, from as early as 2007. To our knowledge the occurrence of mcr-3 and the co-existence of it with bla CTX-M-55 in S. Choleraesuis are reported here for the first time, leading to clinical concern over the treatment of the invasive salmonellosis. This study provides evidence of the potential reservoirs and vectors in the dissemination of the mcr and highlights the co-selection by colistin and/or cephalosporins.
format	article
author	Sirirat Luk-in Tanittha Chatsuwan Naris Kueakulpattana Ubolrat Rirerm Dhammika Leshan Wannigama Rongpong Plongla Ratana Lawung Chaiwat Pulsrikarn Siriporn Chantaroj Pattharaporn Chaichana Nattida Saksaengsopa Teerarat Shanthachol Malee Techapornroong Sunee Chayangsu Wanla Kulwichit
author_facet	Sirirat Luk-in Tanittha Chatsuwan Naris Kueakulpattana Ubolrat Rirerm Dhammika Leshan Wannigama Rongpong Plongla Ratana Lawung Chaiwat Pulsrikarn Siriporn Chantaroj Pattharaporn Chaichana Nattida Saksaengsopa Teerarat Shanthachol Malee Techapornroong Sunee Chayangsu Wanla Kulwichit
author_sort	Sirirat Luk-in
title	Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand
title_short	Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand
title_full	Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand
title_fullStr	Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand
title_full_unstemmed	Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand
title_sort	occurrence of mcr-mediated colistin resistance in salmonella clinical isolates in thailand
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ace9521af3ae4eb08001957d43bbe40e
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Occurrence of mcr-mediated colistin resistance in Salmonella clinical isolates in Thailand

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