Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer

Abstract Peripheral blood mononuclear cells (PBMCs) respond to altered physiological conditions to alleviate the threat. Production of the 70 kDa heat shock protein (HSP70) is up-regulated to protect proteins from degradation. Sequestosome-1 (p62) binds to altered proteins and the p62-protein comple...

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Autores principales:	Theofano Orfanelli, Spyridon Giannopoulos, Eleni Zografos, Aikaterini Athanasiou, Ann Marie Bongiovanni, Georgios Doulaveris, Tracy-Ann Moo, Dayle LaPolla, Chris N. Bakoyiannis, Georgios E. Theodoropoulos, Georgios C. Zografos, Eleni Andreopoulou, Steven S. Witkin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/acee17c0a42341648d5589ed6ac3133e
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spelling	oai:doaj.org-article:acee17c0a42341648d5589ed6ac3133e2021-11-21T12:24:54ZAlterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer10.1038/s41598-021-01683-82045-2322https://doaj.org/article/acee17c0a42341648d5589ed6ac3133e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01683-8https://doaj.org/toc/2045-2322Abstract Peripheral blood mononuclear cells (PBMCs) respond to altered physiological conditions to alleviate the threat. Production of the 70 kDa heat shock protein (HSP70) is up-regulated to protect proteins from degradation. Sequestosome-1 (p62) binds to altered proteins and the p62-protein complex is degraded by autophagy. P62 is also a regulator of intracellular kinase activity and cell differentiation. We hypothesized that the PBMC response to a malignant breast mass involves elevated production of HSP70 and a decrease in intracellular p62. In this study 46 women had their breast mass excised. PBMCs were isolated and intracellular levels of HSP70 and p62 were quantitated by ELISA. Differences between women with a benign or malignant breast mass were determined. A breast malignancy was diagnosed in 38 women (82.6%) while 8 had a benign lesion. Mean intracellular HSP70 levels were 79.3 ng/ml in PBMCs from women with a malignant lesion as opposed to 44.2 ng/ml in controls (p = 0.04). The mean PBMC p62 level was 2.3 ng/ml in women with a benign breast lesion as opposed to 0.6 ng/ml in those with breast cancer (p < 0.001). Mean p62 levels were lowest in women with invasive carcinoma and a positive lymph node biopsy when compared to those with in-situ carcinoma or absence of lymphadenopathy, respectively. Intracellular HSP70 and p62 levels in PBMCs differ between women with a malignant or benign breast lesion. These measurements may be of value in the preoperative triage of women with a breast mass.Theofano OrfanelliSpyridon GiannopoulosEleni ZografosAikaterini AthanasiouAnn Marie BongiovanniGeorgios DoulaverisTracy-Ann MooDayle LaPollaChris N. BakoyiannisGeorgios E. TheodoropoulosGeorgios C. ZografosEleni AndreopoulouSteven S. WitkinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Theofano Orfanelli Spyridon Giannopoulos Eleni Zografos Aikaterini Athanasiou Ann Marie Bongiovanni Georgios Doulaveris Tracy-Ann Moo Dayle LaPolla Chris N. Bakoyiannis Georgios E. Theodoropoulos Georgios C. Zografos Eleni Andreopoulou Steven S. Witkin Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
description	Abstract Peripheral blood mononuclear cells (PBMCs) respond to altered physiological conditions to alleviate the threat. Production of the 70 kDa heat shock protein (HSP70) is up-regulated to protect proteins from degradation. Sequestosome-1 (p62) binds to altered proteins and the p62-protein complex is degraded by autophagy. P62 is also a regulator of intracellular kinase activity and cell differentiation. We hypothesized that the PBMC response to a malignant breast mass involves elevated production of HSP70 and a decrease in intracellular p62. In this study 46 women had their breast mass excised. PBMCs were isolated and intracellular levels of HSP70 and p62 were quantitated by ELISA. Differences between women with a benign or malignant breast mass were determined. A breast malignancy was diagnosed in 38 women (82.6%) while 8 had a benign lesion. Mean intracellular HSP70 levels were 79.3 ng/ml in PBMCs from women with a malignant lesion as opposed to 44.2 ng/ml in controls (p = 0.04). The mean PBMC p62 level was 2.3 ng/ml in women with a benign breast lesion as opposed to 0.6 ng/ml in those with breast cancer (p < 0.001). Mean p62 levels were lowest in women with invasive carcinoma and a positive lymph node biopsy when compared to those with in-situ carcinoma or absence of lymphadenopathy, respectively. Intracellular HSP70 and p62 levels in PBMCs differ between women with a malignant or benign breast lesion. These measurements may be of value in the preoperative triage of women with a breast mass.
format	article
author	Theofano Orfanelli Spyridon Giannopoulos Eleni Zografos Aikaterini Athanasiou Ann Marie Bongiovanni Georgios Doulaveris Tracy-Ann Moo Dayle LaPolla Chris N. Bakoyiannis Georgios E. Theodoropoulos Georgios C. Zografos Eleni Andreopoulou Steven S. Witkin
author_facet	Theofano Orfanelli Spyridon Giannopoulos Eleni Zografos Aikaterini Athanasiou Ann Marie Bongiovanni Georgios Doulaveris Tracy-Ann Moo Dayle LaPolla Chris N. Bakoyiannis Georgios E. Theodoropoulos Georgios C. Zografos Eleni Andreopoulou Steven S. Witkin
author_sort	Theofano Orfanelli
title	Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
title_short	Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
title_full	Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
title_fullStr	Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
title_full_unstemmed	Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
title_sort	alterations of the 70 kda heat shock protein (hsp70) and sequestosome-1 (p62) in women with breast cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/acee17c0a42341648d5589ed6ac3133e
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Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer

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