Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia

BACKGROUND: Diabetes mellitus leads to disruption of the skin repair processes, but the leading mechanisms of this pathology have not yet been identified. In this regard, in our work, we decided to check how hyperglycaemia affects the process of keratinocyte phenotype changes during wound healing....

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Autores principales:	Evgeniy V. Ivanov, Maria P. Morozova, Ekaterina M. Rzhavina, Anna M. Gorbacheva, Svetlana A. Gavrilova, Aleksei K. Erdiakov, Gagik R. Galstyan, Vladimir B. Koshelev
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2020
Materias:	diabetes mellitus streptozotocin reparation wound healing immunohistochemistry cytokeratin 10 cytokeratin 17 Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/acee8a97b6864cc0ae6ce7f9c68e0c6e
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spelling	oai:doaj.org-article:acee8a97b6864cc0ae6ce7f9c68e0c6e2021-11-14T09:00:22ZKeratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia2072-03512072-037810.14341/DM10071https://doaj.org/article/acee8a97b6864cc0ae6ce7f9c68e0c6e2020-04-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/10071https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378BACKGROUND: Diabetes mellitus leads to disruption of the skin repair processes, but the leading mechanisms of this pathology have not yet been identified. In this regard, in our work, we decided to check how hyperglycaemia affects the process of keratinocyte phenotype changes during wound healing. AIMS: To study the effect of hyperglycaemia on wound healing and differentiation of keratinocytes in a rat streptozotocin-induced diabetes model. MATERIALS AND METHODS: Diabetes mellitus was induced in rats by using streptozotocin, 65 mg / kg, intraperitoneally, once. The wound was applied in the supra-scapular region on the 42nd day, after which (after 8, 16, and 24 days) the repair process was evaluated using histological methods. Immunohistochemistry was used to evaluate the expression of cytokeratin-10 and cytokeratin-17. RESULTS: In rats with diabetes mellitus, wound healing slowed down in the later stages, compared with the control group. In general, wound healing was accompanied by an increase in the expression of cytokeratin-10 in its region compared with intact skin, and contractile keratinocytes activation was disrupted in diabetic rat wounds. CONCLUSIONS: Hyperglycaemia slightly slows wound healing in rats and impairs contractile keratinocytes activation.Evgeniy V. IvanovMaria P. MorozovaEkaterina M. RzhavinaAnna M. GorbachevaSvetlana A. GavrilovaAleksei K. ErdiakovGagik R. GalstyanVladimir B. KoshelevEndocrinology Research Centrearticlediabetes mellitusstreptozotocinreparationwound healingimmunohistochemistrycytokeratin 10cytokeratin 17Nutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 23, Iss 1, Pp 19-28 (2020)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	diabetes mellitus streptozotocin reparation wound healing immunohistochemistry cytokeratin 10 cytokeratin 17 Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	diabetes mellitus streptozotocin reparation wound healing immunohistochemistry cytokeratin 10 cytokeratin 17 Nutritional diseases. Deficiency diseases RC620-627 Evgeniy V. Ivanov Maria P. Morozova Ekaterina M. Rzhavina Anna M. Gorbacheva Svetlana A. Gavrilova Aleksei K. Erdiakov Gagik R. Galstyan Vladimir B. Koshelev Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
description	BACKGROUND: Diabetes mellitus leads to disruption of the skin repair processes, but the leading mechanisms of this pathology have not yet been identified. In this regard, in our work, we decided to check how hyperglycaemia affects the process of keratinocyte phenotype changes during wound healing. AIMS: To study the effect of hyperglycaemia on wound healing and differentiation of keratinocytes in a rat streptozotocin-induced diabetes model. MATERIALS AND METHODS: Diabetes mellitus was induced in rats by using streptozotocin, 65 mg / kg, intraperitoneally, once. The wound was applied in the supra-scapular region on the 42nd day, after which (after 8, 16, and 24 days) the repair process was evaluated using histological methods. Immunohistochemistry was used to evaluate the expression of cytokeratin-10 and cytokeratin-17. RESULTS: In rats with diabetes mellitus, wound healing slowed down in the later stages, compared with the control group. In general, wound healing was accompanied by an increase in the expression of cytokeratin-10 in its region compared with intact skin, and contractile keratinocytes activation was disrupted in diabetic rat wounds. CONCLUSIONS: Hyperglycaemia slightly slows wound healing in rats and impairs contractile keratinocytes activation.
format	article
author	Evgeniy V. Ivanov Maria P. Morozova Ekaterina M. Rzhavina Anna M. Gorbacheva Svetlana A. Gavrilova Aleksei K. Erdiakov Gagik R. Galstyan Vladimir B. Koshelev
author_facet	Evgeniy V. Ivanov Maria P. Morozova Ekaterina M. Rzhavina Anna M. Gorbacheva Svetlana A. Gavrilova Aleksei K. Erdiakov Gagik R. Galstyan Vladimir B. Koshelev
author_sort	Evgeniy V. Ivanov
title	Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
title_short	Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
title_full	Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
title_fullStr	Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
title_full_unstemmed	Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
title_sort	keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia
publisher	Endocrinology Research Centre
publishDate	2020
url	https://doaj.org/article/acee8a97b6864cc0ae6ce7f9c68e0c6e
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Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia

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