A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies

Summary: Isolation of viable immune cells from human tissues is critical for the characterization of cellular and molecular processes underlying disease pathogenesis. Here, we describe protocols for the isolation of highly viable immune cells from liver wedges and mesenteric white adipose tissue res...

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Autores principales:	Maria E. Moreno-Fernandez, Michelle S.M.A. Damen, Senad Divanovic
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell isolation Single Cell Flow Cytometry/Mass Cytometry Health Sciences Immunology Metabolism Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/acf24b2036b647e8bf83ce1ccd47cba9
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spelling	oai:doaj.org-article:acf24b2036b647e8bf83ce1ccd47cba92021-11-04T04:39:54ZA protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies2666-166710.1016/j.xpro.2021.100937https://doaj.org/article/acf24b2036b647e8bf83ce1ccd47cba92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006432https://doaj.org/toc/2666-1667Summary: Isolation of viable immune cells from human tissues is critical for the characterization of cellular and molecular processes underlying disease pathogenesis. Here, we describe protocols for the isolation of highly viable immune cells from liver wedges and mesenteric white adipose tissue resections from obese persons. Notably, characterization of the isolated single-immune cell suspensions, via utility of basic immunological interrogations and genetic approaches, promises to generate an improved understanding of altered immunological pathways in obese individuals with or without metabolic diseases.For complete details on the use and execution of this protocol, please refer to Moreno-Fernandez et al. (2021).Maria E. Moreno-FernandezMichelle S.M.A. DamenSenad DivanovicElsevierarticleCell isolationSingle CellFlow Cytometry/Mass CytometryHealth SciencesImmunologyMetabolismScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100937- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell isolation Single Cell Flow Cytometry/Mass Cytometry Health Sciences Immunology Metabolism Science (General) Q1-390
spellingShingle	Cell isolation Single Cell Flow Cytometry/Mass Cytometry Health Sciences Immunology Metabolism Science (General) Q1-390 Maria E. Moreno-Fernandez Michelle S.M.A. Damen Senad Divanovic A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
description	Summary: Isolation of viable immune cells from human tissues is critical for the characterization of cellular and molecular processes underlying disease pathogenesis. Here, we describe protocols for the isolation of highly viable immune cells from liver wedges and mesenteric white adipose tissue resections from obese persons. Notably, characterization of the isolated single-immune cell suspensions, via utility of basic immunological interrogations and genetic approaches, promises to generate an improved understanding of altered immunological pathways in obese individuals with or without metabolic diseases.For complete details on the use and execution of this protocol, please refer to Moreno-Fernandez et al. (2021).
format	article
author	Maria E. Moreno-Fernandez Michelle S.M.A. Damen Senad Divanovic
author_facet	Maria E. Moreno-Fernandez Michelle S.M.A. Damen Senad Divanovic
author_sort	Maria E. Moreno-Fernandez
title	A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
title_short	A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
title_full	A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
title_fullStr	A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
title_full_unstemmed	A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
title_sort	protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/acf24b2036b647e8bf83ce1ccd47cba9
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A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies

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