Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release

Wanfu Men,1 Peiyao Zhu,1 Siyuan Dong,1 Wenke Liu,1 Kun Zhou,1 Yu Bai,1 Xiangli Liu,1 Shulei Gong,1 Can Yang Zhang,2 Shuguang Zhang1 1Department of Thoracic Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, People’s Republic of China; 2Department of Pharma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Men W, Zhu P, Dong S, Liu W, Zhou K, Bai Y, Liu X, Gong S, Zhang CY, Zhang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	pH/redox-sensitive lipid-polymer hybrid drug delivery anticancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/acf4836fa3aa4472ab8533db6213bc9a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:acf4836fa3aa4472ab8533db6213bc9a
record_format	dspace
spelling	oai:doaj.org-article:acf4836fa3aa4472ab8533db6213bc9a2021-12-02T06:41:46ZFabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release1178-2013https://doaj.org/article/acf4836fa3aa4472ab8533db6213bc9a2019-10-01T00:00:00Zhttps://www.dovepress.com/fabrication-of-dual-phredox-responsive-lipid-polymer-hybrid-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wanfu Men,1 Peiyao Zhu,1 Siyuan Dong,1 Wenke Liu,1 Kun Zhou,1 Yu Bai,1 Xiangli Liu,1 Shulei Gong,1 Can Yang Zhang,2 Shuguang Zhang1 1Department of Thoracic Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, People’s Republic of China; 2Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99210, USACorrespondence: Shuguang ZhangDepartment of Thoracic Surgery, The First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning Province, People’s Republic of ChinaTel +86 13909886618Email shgzhang@cmu.edu.cnCan Yang ZhangSingapore-MIT Alliance for Research and Technology, 1 Create Way, #03-12/13/14 Enterprise Wing, Singapore 138602, SingaporeTel +65 9499 0710Email canyang.zhang@smart.mit.eduBackground: The development of biocompatible nanocarriers that can efficiently encapsulate and deliver anticancer drug to the tumor site and provide controlled release of cargos in response to the specific cues for cancer therapy is of great significance.Methods: In this work, dual pH/redox-responsive fabrication of hybrid lipid-polymer nanoparticles (LPNPs) self-assembled from amphiphilic polymer poly(ethylene glycol) methyl ether-grafted disulfide-poly(β-amino esters) (PBAE-ss-mPEG) and PEGylated lipid were prepared and used as drug delivery carriers. The optimization of PEGylated lipid modification was confirmed by analysis of particle size, polydispersity index (PDI), cellular uptake, serum stability, and drug loading capacity. The pKb value of LPNPs was determined as 6.55, indicating the pH-sensitivity. The critical micelle concentration (CMC) values and zeta-potential of LPNPs at different pH values were investigated to confirm its pH-sensitivity. The morphology of LPNPs before and after incubation with reducing agent was imaged to study the redox-responsibility.Results: The in vitro results showed that the drug had controlled release from LPNPs triggered by low pH and high concentration of reducing agent. Furthermore, the cytotoxicity of LPNPs was very low, and the doxorubicin (DOX)-loaded LPNPs could efficiently induce the death of tumor cells in comparison to free DOX.Conclusion: All results demonstrated that the fabricated LPNPs could be potential anticancer drug delivery carriers with a pH/redox-triggered drug release profile, and PEGylated lipid modification might be a useful method to fabricate the drug delivery platform.Keywords: pH-sensitive, redox-sensitive, lipid-polymer, hybrid, drug delivery, anticancer, stimuli-responsivenessMen WZhu PDong SLiu WZhou KBai YLiu XGong SZhang CYZhang SDove Medical PressarticlepH/redox-sensitivelipid-polymerhybriddrug deliveryanticancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8001-8011 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	pH/redox-sensitive lipid-polymer hybrid drug delivery anticancer Medicine (General) R5-920
spellingShingle	pH/redox-sensitive lipid-polymer hybrid drug delivery anticancer Medicine (General) R5-920 Men W Zhu P Dong S Liu W Zhou K Bai Y Liu X Gong S Zhang CY Zhang S Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release
description	Wanfu Men,1 Peiyao Zhu,1 Siyuan Dong,1 Wenke Liu,1 Kun Zhou,1 Yu Bai,1 Xiangli Liu,1 Shulei Gong,1 Can Yang Zhang,2 Shuguang Zhang1 1Department of Thoracic Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, People’s Republic of China; 2Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99210, USACorrespondence: Shuguang ZhangDepartment of Thoracic Surgery, The First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning Province, People’s Republic of ChinaTel +86 13909886618Email shgzhang@cmu.edu.cnCan Yang ZhangSingapore-MIT Alliance for Research and Technology, 1 Create Way, #03-12/13/14 Enterprise Wing, Singapore 138602, SingaporeTel +65 9499 0710Email canyang.zhang@smart.mit.eduBackground: The development of biocompatible nanocarriers that can efficiently encapsulate and deliver anticancer drug to the tumor site and provide controlled release of cargos in response to the specific cues for cancer therapy is of great significance.Methods: In this work, dual pH/redox-responsive fabrication of hybrid lipid-polymer nanoparticles (LPNPs) self-assembled from amphiphilic polymer poly(ethylene glycol) methyl ether-grafted disulfide-poly(β-amino esters) (PBAE-ss-mPEG) and PEGylated lipid were prepared and used as drug delivery carriers. The optimization of PEGylated lipid modification was confirmed by analysis of particle size, polydispersity index (PDI), cellular uptake, serum stability, and drug loading capacity. The pKb value of LPNPs was determined as 6.55, indicating the pH-sensitivity. The critical micelle concentration (CMC) values and zeta-potential of LPNPs at different pH values were investigated to confirm its pH-sensitivity. The morphology of LPNPs before and after incubation with reducing agent was imaged to study the redox-responsibility.Results: The in vitro results showed that the drug had controlled release from LPNPs triggered by low pH and high concentration of reducing agent. Furthermore, the cytotoxicity of LPNPs was very low, and the doxorubicin (DOX)-loaded LPNPs could efficiently induce the death of tumor cells in comparison to free DOX.Conclusion: All results demonstrated that the fabricated LPNPs could be potential anticancer drug delivery carriers with a pH/redox-triggered drug release profile, and PEGylated lipid modification might be a useful method to fabricate the drug delivery platform.Keywords: pH-sensitive, redox-sensitive, lipid-polymer, hybrid, drug delivery, anticancer, stimuli-responsiveness
format	article
author	Men W Zhu P Dong S Liu W Zhou K Bai Y Liu X Gong S Zhang CY Zhang S
author_facet	Men W Zhu P Dong S Liu W Zhou K Bai Y Liu X Gong S Zhang CY Zhang S
author_sort	Men W
title	Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release
title_short	Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release
title_full	Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release
title_fullStr	Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release
title_full_unstemmed	Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release
title_sort	fabrication of dual ph/redox-responsive lipid-polymer hybrid nanoparticles for anticancer drug delivery and controlled release
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/acf4836fa3aa4472ab8533db6213bc9a
work_keys_str_mv	AT menw fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT zhup fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT dongs fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT liuw fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT zhouk fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT baiy fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT liux fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT gongs fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT zhangcy fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease AT zhangs fabricationofdualphredoxresponsivelipidpolymerhybridnanoparticlesforanticancerdrugdeliveryandcontrolledrelease
_version_	1718399771850309632

Fabrication Of Dual pH/redox-Responsive Lipid-Polymer Hybrid Nanoparticles For Anticancer Drug Delivery And Controlled Release

Ejemplares similares