Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice

Midbrain raphe nuclei (MRNs) contain a large number of serotonergic neurons associated with the regulation of numerous types of psychoemotional states and physiological processes. The aim of this work was to study alterations of the MRN transcriptome in mice with prolonged positive or negative fight...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Olga E. Redina, Vladimir N. Babenko, Dmitry A. Smagin, Irina L. Kovalenko, Anna G. Galyamina, Natalia N. Kudryavtseva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	midbrain raphe nuclei transcriptome serotonin corticotropin-releasing hormone thyrotropin-releasing hormone behavior Genetics QH426-470
Acceso en línea:	https://doaj.org/article/acf5d706bf1c4a27b16e4b2750024ba1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:acf5d706bf1c4a27b16e4b2750024ba1
record_format	dspace
spelling	oai:doaj.org-article:acf5d706bf1c4a27b16e4b2750024ba12021-11-25T17:42:19ZCorrelation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice10.3390/genes121118112073-4425https://doaj.org/article/acf5d706bf1c4a27b16e4b2750024ba12021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1811https://doaj.org/toc/2073-4425Midbrain raphe nuclei (MRNs) contain a large number of serotonergic neurons associated with the regulation of numerous types of psychoemotional states and physiological processes. The aim of this work was to study alterations of the MRN transcriptome in mice with prolonged positive or negative fighting experience and to identify key gene networks associated with the regulation of serotonergic system functioning. Numerous genes underwent alterations of transcription in the MRNs of male mice that either manifested aggression or experienced social defeat in daily agonistic interactions. The expression of the <i>Tph2</i> gene encoding the rate-limiting enzyme of the serotonin synthesis pathway correlated with the expression of many genes, 31 of which were common between aggressive and defeated mice and were downregulated in the MRNs of mice of both experimental groups. Among these common differentially expressed genes (DEGs), there were genes associated with behavior, learning, memory, and synaptic signaling. These results suggested that, in the MRNs of the mice, the transcriptome changes associated with serotonergic regulation of various processes are similar between the two groups (aggressive and defeated). In the MRNs, more DEGs correlating with <i>Tph2</i> expression were found in defeated mice than in the winners, which is probably a consequence of deeper <i>Tph2</i> downregulation in the losers. It was shown for the first time that, in both groups of experimental mice, the changes in the transcription of genes controlling the synthesis and transport of serotonin directly correlate with the expression of genes <i>Crh</i> and <i>Trh</i>, which control the synthesis of corticotrophin- and thyrotropin-releasing hormones. Our findings indicate that CRH and TRH locally produced in MRNs are related to serotonergic regulation of brain processes during a chronic social conflict.Olga E. RedinaVladimir N. BabenkoDmitry A. SmaginIrina L. KovalenkoAnna G. GalyaminaNatalia N. KudryavtsevaMDPI AGarticlemidbrain raphe nucleitranscriptomeserotonincorticotropin-releasing hormonethyrotropin-releasing hormonebehaviorGeneticsQH426-470ENGenes, Vol 12, Iss 1811, p 1811 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	midbrain raphe nuclei transcriptome serotonin corticotropin-releasing hormone thyrotropin-releasing hormone behavior Genetics QH426-470
spellingShingle	midbrain raphe nuclei transcriptome serotonin corticotropin-releasing hormone thyrotropin-releasing hormone behavior Genetics QH426-470 Olga E. Redina Vladimir N. Babenko Dmitry A. Smagin Irina L. Kovalenko Anna G. Galyamina Natalia N. Kudryavtseva Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice
description	Midbrain raphe nuclei (MRNs) contain a large number of serotonergic neurons associated with the regulation of numerous types of psychoemotional states and physiological processes. The aim of this work was to study alterations of the MRN transcriptome in mice with prolonged positive or negative fighting experience and to identify key gene networks associated with the regulation of serotonergic system functioning. Numerous genes underwent alterations of transcription in the MRNs of male mice that either manifested aggression or experienced social defeat in daily agonistic interactions. The expression of the <i>Tph2</i> gene encoding the rate-limiting enzyme of the serotonin synthesis pathway correlated with the expression of many genes, 31 of which were common between aggressive and defeated mice and were downregulated in the MRNs of mice of both experimental groups. Among these common differentially expressed genes (DEGs), there were genes associated with behavior, learning, memory, and synaptic signaling. These results suggested that, in the MRNs of the mice, the transcriptome changes associated with serotonergic regulation of various processes are similar between the two groups (aggressive and defeated). In the MRNs, more DEGs correlating with <i>Tph2</i> expression were found in defeated mice than in the winners, which is probably a consequence of deeper <i>Tph2</i> downregulation in the losers. It was shown for the first time that, in both groups of experimental mice, the changes in the transcription of genes controlling the synthesis and transport of serotonin directly correlate with the expression of genes <i>Crh</i> and <i>Trh</i>, which control the synthesis of corticotrophin- and thyrotropin-releasing hormones. Our findings indicate that CRH and TRH locally produced in MRNs are related to serotonergic regulation of brain processes during a chronic social conflict.
format	article
author	Olga E. Redina Vladimir N. Babenko Dmitry A. Smagin Irina L. Kovalenko Anna G. Galyamina Natalia N. Kudryavtseva
author_facet	Olga E. Redina Vladimir N. Babenko Dmitry A. Smagin Irina L. Kovalenko Anna G. Galyamina Natalia N. Kudryavtseva
author_sort	Olga E. Redina
title	Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice
title_short	Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice
title_full	Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice
title_fullStr	Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice
title_full_unstemmed	Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice
title_sort	correlation of expression changes between genes controlling 5-ht synthesis and genes <i>crh</i> and <i>trh</i> in the midbrain raphe nuclei of chronically aggressive and defeated male mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/acf5d706bf1c4a27b16e4b2750024ba1
work_keys_str_mv	AT olgaeredina correlationofexpressionchangesbetweengenescontrolling5htsynthesisandgenesicrhianditrhiinthemidbrainraphenucleiofchronicallyaggressiveanddefeatedmalemice AT vladimirnbabenko correlationofexpressionchangesbetweengenescontrolling5htsynthesisandgenesicrhianditrhiinthemidbrainraphenucleiofchronicallyaggressiveanddefeatedmalemice AT dmitryasmagin correlationofexpressionchangesbetweengenescontrolling5htsynthesisandgenesicrhianditrhiinthemidbrainraphenucleiofchronicallyaggressiveanddefeatedmalemice AT irinalkovalenko correlationofexpressionchangesbetweengenescontrolling5htsynthesisandgenesicrhianditrhiinthemidbrainraphenucleiofchronicallyaggressiveanddefeatedmalemice AT annaggalyamina correlationofexpressionchangesbetweengenescontrolling5htsynthesisandgenesicrhianditrhiinthemidbrainraphenucleiofchronicallyaggressiveanddefeatedmalemice AT nataliankudryavtseva correlationofexpressionchangesbetweengenescontrolling5htsynthesisandgenesicrhianditrhiinthemidbrainraphenucleiofchronicallyaggressiveanddefeatedmalemice
_version_	1718412124744581120

Correlation of Expression Changes between Genes Controlling 5-HT Synthesis and Genes <i>Crh</i> and <i>Trh</i> in the Midbrain Raphe Nuclei of Chronically Aggressive and Defeated Male Mice

Ejemplares similares