Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate <i>Amphidinium carterae</i> strain LACW11, isolated from the west of Ireland. Amphidinolides have bee...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/acf6011d52b04bd49b8c66b7846a999b |
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| Sumario: | Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate <i>Amphidinium carterae</i> strain LACW11, isolated from the west of Ireland. Amphidinolides have been identified as cytotoxic polyoxygenated polyketides produced by several <i>Amphidinium</i> species. Phylogenetic inference assigned our strain to <i>Amphidinium carterae</i> subclade III, along with isolates interspersed in different geographic regions. A two-stage extraction and fractionation process of the biomass was carried out. Extracts obtained after stage-1 were tested for bioactivity against bacterial ATCC strains of <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>. The stage-2 solid phase extraction provided 16 fractions, which were tested against <i>S. aureus</i> and <i>E. faecalis.</i> Fractions I, J and K yielded minimum inhibitory concentrations between 16 μg/mL and 256 μg/mL for both Gram-positive. A targeted metabolomic approach using UHPLC-HRMS/MS analysis applied on fractions G to J evidenced the presence of amphidinol type compounds AM-A, AM-B, AM-22 and a new derivative dehydroAM-A, with characteristic masses of <i>m/z</i> 1361, 1463, 1667 and 1343, respectively. Combining the results of the biological assays with the targeted metabolomic approach, we could conclude that AM-A and the new derivative dehydroAM-A are responsible for the detected antimicrobial bioactivity. |
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