Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)

Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate <i>Amphidinium carterae</i> strain LACW11, isolated from the west of Ireland. Amphidinolides have bee...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria Elena Barone, Elliot Murphy, Rachel Parkes, Gerard T. A. Fleming, Floriana Campanile, Olivier P. Thomas, Nicolas Touzet
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microalgae <i>Amphidinium carterae</i> antimicrobial activity marine natural products (MNP) minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/acf6011d52b04bd49b8c66b7846a999b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:acf6011d52b04bd49b8c66b7846a999b
record_format	dspace
spelling	oai:doaj.org-article:acf6011d52b04bd49b8c66b7846a999b2021-11-25T17:54:12ZAntibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)10.3390/ijms2222121961422-00671661-6596https://doaj.org/article/acf6011d52b04bd49b8c66b7846a999b2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12196https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate <i>Amphidinium carterae</i> strain LACW11, isolated from the west of Ireland. Amphidinolides have been identified as cytotoxic polyoxygenated polyketides produced by several <i>Amphidinium</i> species. Phylogenetic inference assigned our strain to <i>Amphidinium carterae</i> subclade III, along with isolates interspersed in different geographic regions. A two-stage extraction and fractionation process of the biomass was carried out. Extracts obtained after stage-1 were tested for bioactivity against bacterial ATCC strains of <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>. The stage-2 solid phase extraction provided 16 fractions, which were tested against <i>S. aureus</i> and <i>E. faecalis.</i> Fractions I, J and K yielded minimum inhibitory concentrations between 16 μg/mL and 256 μg/mL for both Gram-positive. A targeted metabolomic approach using UHPLC-HRMS/MS analysis applied on fractions G to J evidenced the presence of amphidinol type compounds AM-A, AM-B, AM-22 and a new derivative dehydroAM-A, with characteristic masses of <i>m/z</i> 1361, 1463, 1667 and 1343, respectively. Combining the results of the biological assays with the targeted metabolomic approach, we could conclude that AM-A and the new derivative dehydroAM-A are responsible for the detected antimicrobial bioactivity.Maria Elena BaroneElliot MurphyRachel ParkesGerard T. A. FlemingFloriana CampanileOlivier P. ThomasNicolas TouzetMDPI AGarticlemicroalgae<i>Amphidinium carterae</i>antimicrobial activitymarine natural products (MNP)minimum inhibitory concentration (MIC)minimum bactericidal concentration (MBC)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12196, p 12196 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microalgae <i>Amphidinium carterae</i> antimicrobial activity marine natural products (MNP) minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	microalgae <i>Amphidinium carterae</i> antimicrobial activity marine natural products (MNP) minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) Biology (General) QH301-705.5 Chemistry QD1-999 Maria Elena Barone Elliot Murphy Rachel Parkes Gerard T. A. Fleming Floriana Campanile Olivier P. Thomas Nicolas Touzet Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
description	Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate <i>Amphidinium carterae</i> strain LACW11, isolated from the west of Ireland. Amphidinolides have been identified as cytotoxic polyoxygenated polyketides produced by several <i>Amphidinium</i> species. Phylogenetic inference assigned our strain to <i>Amphidinium carterae</i> subclade III, along with isolates interspersed in different geographic regions. A two-stage extraction and fractionation process of the biomass was carried out. Extracts obtained after stage-1 were tested for bioactivity against bacterial ATCC strains of <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>. The stage-2 solid phase extraction provided 16 fractions, which were tested against <i>S. aureus</i> and <i>E. faecalis.</i> Fractions I, J and K yielded minimum inhibitory concentrations between 16 μg/mL and 256 μg/mL for both Gram-positive. A targeted metabolomic approach using UHPLC-HRMS/MS analysis applied on fractions G to J evidenced the presence of amphidinol type compounds AM-A, AM-B, AM-22 and a new derivative dehydroAM-A, with characteristic masses of <i>m/z</i> 1361, 1463, 1667 and 1343, respectively. Combining the results of the biological assays with the targeted metabolomic approach, we could conclude that AM-A and the new derivative dehydroAM-A are responsible for the detected antimicrobial bioactivity.
format	article
author	Maria Elena Barone Elliot Murphy Rachel Parkes Gerard T. A. Fleming Floriana Campanile Olivier P. Thomas Nicolas Touzet
author_facet	Maria Elena Barone Elliot Murphy Rachel Parkes Gerard T. A. Fleming Floriana Campanile Olivier P. Thomas Nicolas Touzet
author_sort	Maria Elena Barone
title	Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
title_short	Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
title_full	Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
title_fullStr	Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
title_full_unstemmed	Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)
title_sort	antibacterial activity and amphidinol profiling of the marine dinoflagellate <i>amphidinium carterae</i> (subclade iii)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/acf6011d52b04bd49b8c66b7846a999b
work_keys_str_mv	AT mariaelenabarone antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii AT elliotmurphy antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii AT rachelparkes antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii AT gerardtafleming antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii AT florianacampanile antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii AT olivierpthomas antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii AT nicolastouzet antibacterialactivityandamphidinolprofilingofthemarinedinoflagellateiamphidiniumcarteraeisubcladeiii
_version_	1718411880264892416

Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate <i>Amphidinium carterae</i> (Subclade III)

Ejemplares similares