smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.

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Detalles Bibliográficos
Autores principales:	Llilians Calvo, Matthew Ronshaugen, Tom Pettini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/acfc07cd23d04e13bfe55c8b2d03240f
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spelling	oai:doaj.org-article:acfc07cd23d04e13bfe55c8b2d03240f2021-12-02T16:30:30ZsmiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods10.1038/s42003-021-01803-02399-3642https://doaj.org/article/acfc07cd23d04e13bfe55c8b2d03240f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01803-0https://doaj.org/toc/2399-3642Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.Llilians CalvoMatthew RonshaugenTom PettiniNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Llilians Calvo Matthew Ronshaugen Tom Pettini smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
description	Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.
format	article
author	Llilians Calvo Matthew Ronshaugen Tom Pettini
author_facet	Llilians Calvo Matthew Ronshaugen Tom Pettini
author_sort	Llilians Calvo
title	smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_short	smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_full	smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_fullStr	smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_full_unstemmed	smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_sort	smifish and embryo segmentation for single-cell multi-gene rna quantification in arthropods
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/acfc07cd23d04e13bfe55c8b2d03240f
work_keys_str_mv	AT llilianscalvo smifishandembryosegmentationforsinglecellmultigenernaquantificationinarthropods AT matthewronshaugen smifishandembryosegmentationforsinglecellmultigenernaquantificationinarthropods AT tompettini smifishandembryosegmentationforsinglecellmultigenernaquantificationinarthropods
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smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

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