smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.

Guardado en:
Detalles Bibliográficos
Autores principales: Llilians Calvo, Matthew Ronshaugen, Tom Pettini
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Acceso en línea:https://doaj.org/article/acfc07cd23d04e13bfe55c8b2d03240f
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares