Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.

Noninvasive techniques for evaluating the severity of nonalcoholic fatty liver disease (NAFLD) have shown limited diagnostic performance. MicroRNAs (miRNAs) are useful biomarkers for diagnosing and monitoring the progression and treatment response to several diseases. Here, we evaluated whether seru...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jeong-An Gim, Soo Min Bang, Young-Sun Lee, Yoonseok Lee, Sun Young Yim, Young Kul Jung, Hayeon Kim, Baek-Hui Kim, Ji Hoon Kim, Yeon Seok Seo, Hyung Joon Yim, Jong Eun Yeon, Soon Ho Um, Kwan Soo Byun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/acfd4b5b16514c89aa687b33c2923987
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:acfd4b5b16514c89aa687b33c2923987
record_format	dspace
spelling	oai:doaj.org-article:acfd4b5b16514c89aa687b33c29239872021-12-02T20:18:33ZEvaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.1932-620310.1371/journal.pone.0255822https://doaj.org/article/acfd4b5b16514c89aa687b33c29239872021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255822https://doaj.org/toc/1932-6203Noninvasive techniques for evaluating the severity of nonalcoholic fatty liver disease (NAFLD) have shown limited diagnostic performance. MicroRNAs (miRNAs) are useful biomarkers for diagnosing and monitoring the progression and treatment response to several diseases. Here, we evaluated whether serum exosomal miRNAs could be used for the diagnosis and prognosis of NAFLD severity. Exosomal miRNAs were isolated from the sera of 41 patients with NAFLD (diagnosed using liver biopsy) for microarray profiling. The degree of NAFLD severity was determined using inflammation, steatosis, and ballooning scores and the NAFLD activity score (NAS). Correlations between miRNA expression, clinical and biochemical parameters, and mRNA expression were analyzed. Overall, 25, 11, 13, and 14 miRNAs correlated with the inflammation score, steatosis score, ballooning score, and NAS, respectively, with 33 significant correlations observed between 27 miRNAs and six clinical variables. Eight miRNAs (let-7b-5p, miR-378h, -1184, -3613-3p, -877-5p, -602, -133b, and 509-3p) showed anticorrelated patterns with the corresponding mRNA expression. In fibrosis, 52 and 30 interactions corresponding to high miRNA-low mRNA and low miRNA-high mRNA expression, respectively, were observed. The present results therefore suggest that serum exosomal miRNAs can be used to evaluate NAFLD severity and identify potential targets for NAFLD treatment.Jeong-An GimSoo Min BangYoung-Sun LeeYoonseok LeeSun Young YimYoung Kul JungHayeon KimBaek-Hui KimJi Hoon KimYeon Seok SeoHyung Joon YimJong Eun YeonSoon Ho UmKwan Soo ByunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255822 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeong-An Gim Soo Min Bang Young-Sun Lee Yoonseok Lee Sun Young Yim Young Kul Jung Hayeon Kim Baek-Hui Kim Ji Hoon Kim Yeon Seok Seo Hyung Joon Yim Jong Eun Yeon Soon Ho Um Kwan Soo Byun Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.
description	Noninvasive techniques for evaluating the severity of nonalcoholic fatty liver disease (NAFLD) have shown limited diagnostic performance. MicroRNAs (miRNAs) are useful biomarkers for diagnosing and monitoring the progression and treatment response to several diseases. Here, we evaluated whether serum exosomal miRNAs could be used for the diagnosis and prognosis of NAFLD severity. Exosomal miRNAs were isolated from the sera of 41 patients with NAFLD (diagnosed using liver biopsy) for microarray profiling. The degree of NAFLD severity was determined using inflammation, steatosis, and ballooning scores and the NAFLD activity score (NAS). Correlations between miRNA expression, clinical and biochemical parameters, and mRNA expression were analyzed. Overall, 25, 11, 13, and 14 miRNAs correlated with the inflammation score, steatosis score, ballooning score, and NAS, respectively, with 33 significant correlations observed between 27 miRNAs and six clinical variables. Eight miRNAs (let-7b-5p, miR-378h, -1184, -3613-3p, -877-5p, -602, -133b, and 509-3p) showed anticorrelated patterns with the corresponding mRNA expression. In fibrosis, 52 and 30 interactions corresponding to high miRNA-low mRNA and low miRNA-high mRNA expression, respectively, were observed. The present results therefore suggest that serum exosomal miRNAs can be used to evaluate NAFLD severity and identify potential targets for NAFLD treatment.
format	article
author	Jeong-An Gim Soo Min Bang Young-Sun Lee Yoonseok Lee Sun Young Yim Young Kul Jung Hayeon Kim Baek-Hui Kim Ji Hoon Kim Yeon Seok Seo Hyung Joon Yim Jong Eun Yeon Soon Ho Um Kwan Soo Byun
author_facet	Jeong-An Gim Soo Min Bang Young-Sun Lee Yoonseok Lee Sun Young Yim Young Kul Jung Hayeon Kim Baek-Hui Kim Ji Hoon Kim Yeon Seok Seo Hyung Joon Yim Jong Eun Yeon Soon Ho Um Kwan Soo Byun
author_sort	Jeong-An Gim
title	Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.
title_short	Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.
title_full	Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.
title_fullStr	Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.
title_full_unstemmed	Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.
title_sort	evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal mirna expression.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/acfd4b5b16514c89aa687b33c2923987
work_keys_str_mv	AT jeongangim evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT soominbang evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT youngsunlee evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT yoonseoklee evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT sunyoungyim evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT youngkuljung evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT hayeonkim evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT baekhuikim evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT jihoonkim evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT yeonseokseo evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT hyungjoonyim evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT jongeunyeon evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT soonhoum evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression AT kwansoobyun evaluationoftheseverityofnonalcoholicfattyliverdiseasethroughanalysisofserumexosomalmirnaexpression
_version_	1718374304182173696

Evaluation of the severity of nonalcoholic fatty liver disease through analysis of serum exosomal miRNA expression.

Ejemplares similares