STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena

Abstract The base of the cilium comprising the transition zone (TZ) and transition fibers (TF) acts as a selecting gate to regulate the intraflagellar transport (IFT)-dependent trafficking of proteins to and from cilia. Before entering the ciliary compartment, IFT complexes and transported cargoes a...

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Autores principales:	Khodor S. Hazime, Zhu Zhou, Ewa Joachimiak, Natalia A. Bulgakova, Dorota Wloga, Jarema J. Malicki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad03647e47844e35a88866b74e826023
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spelling	oai:doaj.org-article:ad03647e47844e35a88866b74e8260232021-12-02T14:26:20ZSTORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena10.1038/s41598-021-86909-52045-2322https://doaj.org/article/ad03647e47844e35a88866b74e8260232021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86909-5https://doaj.org/toc/2045-2322Abstract The base of the cilium comprising the transition zone (TZ) and transition fibers (TF) acts as a selecting gate to regulate the intraflagellar transport (IFT)-dependent trafficking of proteins to and from cilia. Before entering the ciliary compartment, IFT complexes and transported cargoes accumulate at or near the base of the cilium. The spatial organization of IFT proteins at the cilia base is key for understanding cilia formation and function. Using stochastic optical reconstruction microscopy (STORM) and computational averaging, we show that seven TZ, nine IFT, three Bardet–Biedl syndrome (BBS), and one centrosomal protein, form 9-clustered rings at the cilium base of a ciliate Tetrahymena thermophila. In the axial dimension, analyzed TZ proteins localize to a narrow region of about 30 nm while IFT proteins dock approximately 80 nm proximal to TZ. Moreover, the IFT-A subcomplex is positioned peripheral to the IFT-B subcomplex and the investigated BBS proteins localize near the ciliary membrane. The positioning of the HA-tagged N- and C-termini of the selected proteins enabled the prediction of the spatial orientation of protein particles and likely cargo interaction sites. Based on the obtained data, we built a comprehensive 3D-model showing the arrangement of the investigated ciliary proteins.Khodor S. HazimeZhu ZhouEwa JoachimiakNatalia A. BulgakovaDorota WlogaJarema J. MalickiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khodor S. Hazime Zhu Zhou Ewa Joachimiak Natalia A. Bulgakova Dorota Wloga Jarema J. Malicki STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena
description	Abstract The base of the cilium comprising the transition zone (TZ) and transition fibers (TF) acts as a selecting gate to regulate the intraflagellar transport (IFT)-dependent trafficking of proteins to and from cilia. Before entering the ciliary compartment, IFT complexes and transported cargoes accumulate at or near the base of the cilium. The spatial organization of IFT proteins at the cilia base is key for understanding cilia formation and function. Using stochastic optical reconstruction microscopy (STORM) and computational averaging, we show that seven TZ, nine IFT, three Bardet–Biedl syndrome (BBS), and one centrosomal protein, form 9-clustered rings at the cilium base of a ciliate Tetrahymena thermophila. In the axial dimension, analyzed TZ proteins localize to a narrow region of about 30 nm while IFT proteins dock approximately 80 nm proximal to TZ. Moreover, the IFT-A subcomplex is positioned peripheral to the IFT-B subcomplex and the investigated BBS proteins localize near the ciliary membrane. The positioning of the HA-tagged N- and C-termini of the selected proteins enabled the prediction of the spatial orientation of protein particles and likely cargo interaction sites. Based on the obtained data, we built a comprehensive 3D-model showing the arrangement of the investigated ciliary proteins.
format	article
author	Khodor S. Hazime Zhu Zhou Ewa Joachimiak Natalia A. Bulgakova Dorota Wloga Jarema J. Malicki
author_facet	Khodor S. Hazime Zhu Zhou Ewa Joachimiak Natalia A. Bulgakova Dorota Wloga Jarema J. Malicki
author_sort	Khodor S. Hazime
title	STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena
title_short	STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena
title_full	STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena
title_fullStr	STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena
title_full_unstemmed	STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena
title_sort	storm imaging reveals the spatial arrangement of transition zone components and ift particles at the ciliary base in tetrahymena
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ad03647e47844e35a88866b74e826023
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STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena

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