Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains

Human hepatitis Delta virus (HDV) infection is associated to the most severe viral hepatic disease, including severe acute liver decompensation and progression to cirrhosis, and hepatocellular carcinoma. HDV is a satellite of hepatitis B virus (HBV) that requires the HBV envelope proteins for assemb...

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Autores principales:	Athenaïs Gerber, Frédéric Le Gal, Samira Dziri, Chakib Alloui, Dominique Roulot, Paul Dény, Camille Sureau, Ségolène Brichler, Emmanuel Gordien
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	HDV editing genotype HDAg next-generation-sequencing pathogenesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ad04e6d50b8546aab274f48cc0a54666
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spelling	oai:doaj.org-article:ad04e6d50b8546aab274f48cc0a546662021-11-16T07:44:00ZComprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains1664-302X10.3389/fmicb.2021.751531https://doaj.org/article/ad04e6d50b8546aab274f48cc0a546662021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.751531/fullhttps://doaj.org/toc/1664-302XHuman hepatitis Delta virus (HDV) infection is associated to the most severe viral hepatic disease, including severe acute liver decompensation and progression to cirrhosis, and hepatocellular carcinoma. HDV is a satellite of hepatitis B virus (HBV) that requires the HBV envelope proteins for assembly of HDV virions. HDV and HBV exhibit a large genetic diversity that extends, respectively to eight (HDV-1 to -8) and to ten (HBV/A to/J) genotypes. Molecular determinants of HDV virion assembly consist of a C-terminal Proline-rich domain in the large Hepatitis Delta Antigen (HDAg) protein, also known as the Delta packaging domain (DPD) and of a Tryptophan-rich domain, the HDV matrix domain (HMD) in the C-terminal region of the HBV envelope proteins. In this study, we performed a systematic genotyping of HBV and HDV in a cohort 1,590 HDV-RNA-positive serum samples collected between 2001 to 2014, from patients originated from diverse parts of the world, thus reflecting a large genetic diversity. Among these samples, 526 HBV (HBV/A, B, C, D, E, and G) and HDV (HDV-1, 2, 3, and 5 to -8) genotype couples could be obtained. We provide results of a comprehensive analysis of the amino-acid sequence conservation within the HMD and structural and functional features of the DPD that may account for the yet optimal interactions between HDV and its helper HBV.Athenaïs GerberAthenaïs GerberFrédéric Le GalFrédéric Le GalFrédéric Le GalSamira DziriSamira DziriChakib AllouiChakib AllouiChakib AllouiDominique RoulotDominique RoulotDominique RoulotPaul DényPaul DényCamille SureauSégolène BrichlerSégolène BrichlerSégolène BrichlerEmmanuel GordienEmmanuel GordienEmmanuel GordienFrontiers Media S.A.articleHDVeditinggenotypeHDAgnext-generation-sequencingpathogenesisMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HDV editing genotype HDAg next-generation-sequencing pathogenesis Microbiology QR1-502
spellingShingle	HDV editing genotype HDAg next-generation-sequencing pathogenesis Microbiology QR1-502 Athenaïs Gerber Athenaïs Gerber Frédéric Le Gal Frédéric Le Gal Frédéric Le Gal Samira Dziri Samira Dziri Chakib Alloui Chakib Alloui Chakib Alloui Dominique Roulot Dominique Roulot Dominique Roulot Paul Dény Paul Dény Camille Sureau Ségolène Brichler Ségolène Brichler Ségolène Brichler Emmanuel Gordien Emmanuel Gordien Emmanuel Gordien Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains
description	Human hepatitis Delta virus (HDV) infection is associated to the most severe viral hepatic disease, including severe acute liver decompensation and progression to cirrhosis, and hepatocellular carcinoma. HDV is a satellite of hepatitis B virus (HBV) that requires the HBV envelope proteins for assembly of HDV virions. HDV and HBV exhibit a large genetic diversity that extends, respectively to eight (HDV-1 to -8) and to ten (HBV/A to/J) genotypes. Molecular determinants of HDV virion assembly consist of a C-terminal Proline-rich domain in the large Hepatitis Delta Antigen (HDAg) protein, also known as the Delta packaging domain (DPD) and of a Tryptophan-rich domain, the HDV matrix domain (HMD) in the C-terminal region of the HBV envelope proteins. In this study, we performed a systematic genotyping of HBV and HDV in a cohort 1,590 HDV-RNA-positive serum samples collected between 2001 to 2014, from patients originated from diverse parts of the world, thus reflecting a large genetic diversity. Among these samples, 526 HBV (HBV/A, B, C, D, E, and G) and HDV (HDV-1, 2, 3, and 5 to -8) genotype couples could be obtained. We provide results of a comprehensive analysis of the amino-acid sequence conservation within the HMD and structural and functional features of the DPD that may account for the yet optimal interactions between HDV and its helper HBV.
format	article
author	Athenaïs Gerber Athenaïs Gerber Frédéric Le Gal Frédéric Le Gal Frédéric Le Gal Samira Dziri Samira Dziri Chakib Alloui Chakib Alloui Chakib Alloui Dominique Roulot Dominique Roulot Dominique Roulot Paul Dény Paul Dény Camille Sureau Ségolène Brichler Ségolène Brichler Ségolène Brichler Emmanuel Gordien Emmanuel Gordien Emmanuel Gordien
author_facet	Athenaïs Gerber Athenaïs Gerber Frédéric Le Gal Frédéric Le Gal Frédéric Le Gal Samira Dziri Samira Dziri Chakib Alloui Chakib Alloui Chakib Alloui Dominique Roulot Dominique Roulot Dominique Roulot Paul Dény Paul Dény Camille Sureau Ségolène Brichler Ségolène Brichler Ségolène Brichler Emmanuel Gordien Emmanuel Gordien Emmanuel Gordien
author_sort	Athenaïs Gerber
title	Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains
title_short	Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains
title_full	Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains
title_fullStr	Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains
title_full_unstemmed	Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains
title_sort	comprehensive analysis of hepatitis delta virus assembly determinants according to genotypes: lessons from a study of 526 hepatitis delta virus clinical strains
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ad04e6d50b8546aab274f48cc0a54666
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Comprehensive Analysis of Hepatitis Delta Virus Assembly Determinants According to Genotypes: Lessons From a Study of 526 Hepatitis Delta Virus Clinical Strains

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