Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
Abstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and poro...
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2021
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oai:doaj.org-article:ad07450ce7e747b1a16fc8a565e8bf6e2021-12-02T18:49:21ZConfocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces10.1038/s41598-021-94939-22045-2322https://doaj.org/article/ad07450ce7e747b1a16fc8a565e8bf6e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94939-2https://doaj.org/toc/2045-2322Abstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and porous surface contamination. Confocal spectral laser microscope (CSLM) was employed to characterize and use the autofluorescence of Sessile oak (Quercus petraea), Douglas fir (Pseudotsuga menziesii) and poplar (Populus euramericana alba L.) wood discs cut into transversal (RT) and tangential (LT) planes. The red fluorescent area occupied by bacteria was differentiated from that of wood, which represented the bacterial quantification, survival and bio-distribution on surfaces from one hour to one week after inoculation. More bacteria were present near the surface on LT face wood as compared to RT and they persisted throughout the study period. Furthermore, this innovative methodology identified that S. aureus formed a dense biofilm on melamine but not on oak wood in similar inoculation and growth conditions. Conclusively, the endogenous fluorescence of materials and the model bacterium permitted direct quantification of surface contamination by using CSLM and it is a promising tool for hygienic safety evaluation.Muhammad Tanveer MunirNattar ManeewanJulien PichonMohammed GharbiaIsmael Oumarou-MahamaneJessica BaudeChantal ThorinDidier LepelletierPatrice Le PapeMatthieu EveillardMark IrleHélène PailhorièsFlorence AviatChristophe BelloncleMichel FederighiLaurence DubreilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Muhammad Tanveer Munir Nattar Maneewan Julien Pichon Mohammed Gharbia Ismael Oumarou-Mahamane Jessica Baude Chantal Thorin Didier Lepelletier Patrice Le Pape Matthieu Eveillard Mark Irle Hélène Pailhoriès Florence Aviat Christophe Belloncle Michel Federighi Laurence Dubreil Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces |
| description |
Abstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and porous surface contamination. Confocal spectral laser microscope (CSLM) was employed to characterize and use the autofluorescence of Sessile oak (Quercus petraea), Douglas fir (Pseudotsuga menziesii) and poplar (Populus euramericana alba L.) wood discs cut into transversal (RT) and tangential (LT) planes. The red fluorescent area occupied by bacteria was differentiated from that of wood, which represented the bacterial quantification, survival and bio-distribution on surfaces from one hour to one week after inoculation. More bacteria were present near the surface on LT face wood as compared to RT and they persisted throughout the study period. Furthermore, this innovative methodology identified that S. aureus formed a dense biofilm on melamine but not on oak wood in similar inoculation and growth conditions. Conclusively, the endogenous fluorescence of materials and the model bacterium permitted direct quantification of surface contamination by using CSLM and it is a promising tool for hygienic safety evaluation. |
| format |
article |
| author |
Muhammad Tanveer Munir Nattar Maneewan Julien Pichon Mohammed Gharbia Ismael Oumarou-Mahamane Jessica Baude Chantal Thorin Didier Lepelletier Patrice Le Pape Matthieu Eveillard Mark Irle Hélène Pailhoriès Florence Aviat Christophe Belloncle Michel Federighi Laurence Dubreil |
| author_facet |
Muhammad Tanveer Munir Nattar Maneewan Julien Pichon Mohammed Gharbia Ismael Oumarou-Mahamane Jessica Baude Chantal Thorin Didier Lepelletier Patrice Le Pape Matthieu Eveillard Mark Irle Hélène Pailhoriès Florence Aviat Christophe Belloncle Michel Federighi Laurence Dubreil |
| author_sort |
Muhammad Tanveer Munir |
| title |
Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces |
| title_short |
Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces |
| title_full |
Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces |
| title_fullStr |
Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces |
| title_full_unstemmed |
Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces |
| title_sort |
confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mcherry staphylococcus aureus on solid surfaces |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/ad07450ce7e747b1a16fc8a565e8bf6e |
| work_keys_str_mv |
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