CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.

Human mesenchymal stem cells (hMSCs) display immunosuppressive properties in vitro and the potential has also been transferred successfully to clinical trials for treatment of autoimmune diseases. OX-2 (CD200), a member of the immunoglobulin superfamily, is widely expressed in several tissues and ha...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mika Pietilä, Siri Lehtonen, Elina Tuovinen, Kaarina Lähteenmäki, Saara Laitinen, Hannu-Ville Leskelä, Antti Nätynki, Juha Pesälä, Katrina Nordström, Petri Lehenkari
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad08aae4cac149c496e2f344c16298e0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad08aae4cac149c496e2f344c16298e0
record_format	dspace
spelling	oai:doaj.org-article:ad08aae4cac149c496e2f344c16298e02021-11-18T07:27:30ZCD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.1932-620310.1371/journal.pone.0031671https://doaj.org/article/ad08aae4cac149c496e2f344c16298e02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363701/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Human mesenchymal stem cells (hMSCs) display immunosuppressive properties in vitro and the potential has also been transferred successfully to clinical trials for treatment of autoimmune diseases. OX-2 (CD200), a member of the immunoglobulin superfamily, is widely expressed in several tissues and has recently been found from hMSCs. The CD200 receptor (CD200R) occurs only in myeloid-lineage cells. The CD200-CD200R is involved in down-regulation of several immune cells, especially macrophages. The present study on 20 hMSC lines shows that the CD200 expression pattern varied from high (CD200Hi) to medium (CD200Me) and low (CD200Lo) in bone marrow-derived mesenchymal stem cell (BMMSC) lines, whereas umbilical cord blood derived mesenchymal stem cells (UCBMSCs) were constantly negative for CD200. The role of the CD200-CD200R axis in BMMSCs mediated immunosuppression was studied using THP-1 human macrophages. Interestingly, hMSCs showed greater inhibition of TNF-α secretion in co-cultures with IFN-γ primed THP-1 macrophages when compared to LPS activated cells. The ability of CD200Hi BMMSCs to suppress TNF-α secretion from IFN-γ stimulated THP-1 macrophages was significantly greater when compared to CD200Lo whereas UCBMSCs did not significantly reduce TNF-α secretion. The interference of CD200 binding to the CD200R by anti-CD200 antibody weakened the capability of BMMSCs to inhibit TNF-α secretion from IFN-γ activated THP-1 macrophages. This study clearly demonstrated that the efficiency of BMMSCs to suppress TNF-α secretion of THP-1 macrophages was dependent on the type of stimulus. Moreover, the CD200-CD200r axis could have a previously unidentified role in the BMMSC mediated immunosuppression.Mika PietiläSiri LehtonenElina TuovinenKaarina LähteenmäkiSaara LaitinenHannu-Ville LeskeläAntti NätynkiJuha PesäläKatrina NordströmPetri LehenkariPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31671 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mika Pietilä Siri Lehtonen Elina Tuovinen Kaarina Lähteenmäki Saara Laitinen Hannu-Ville Leskelä Antti Nätynki Juha Pesälä Katrina Nordström Petri Lehenkari CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
description	Human mesenchymal stem cells (hMSCs) display immunosuppressive properties in vitro and the potential has also been transferred successfully to clinical trials for treatment of autoimmune diseases. OX-2 (CD200), a member of the immunoglobulin superfamily, is widely expressed in several tissues and has recently been found from hMSCs. The CD200 receptor (CD200R) occurs only in myeloid-lineage cells. The CD200-CD200R is involved in down-regulation of several immune cells, especially macrophages. The present study on 20 hMSC lines shows that the CD200 expression pattern varied from high (CD200Hi) to medium (CD200Me) and low (CD200Lo) in bone marrow-derived mesenchymal stem cell (BMMSC) lines, whereas umbilical cord blood derived mesenchymal stem cells (UCBMSCs) were constantly negative for CD200. The role of the CD200-CD200R axis in BMMSCs mediated immunosuppression was studied using THP-1 human macrophages. Interestingly, hMSCs showed greater inhibition of TNF-α secretion in co-cultures with IFN-γ primed THP-1 macrophages when compared to LPS activated cells. The ability of CD200Hi BMMSCs to suppress TNF-α secretion from IFN-γ stimulated THP-1 macrophages was significantly greater when compared to CD200Lo whereas UCBMSCs did not significantly reduce TNF-α secretion. The interference of CD200 binding to the CD200R by anti-CD200 antibody weakened the capability of BMMSCs to inhibit TNF-α secretion from IFN-γ activated THP-1 macrophages. This study clearly demonstrated that the efficiency of BMMSCs to suppress TNF-α secretion of THP-1 macrophages was dependent on the type of stimulus. Moreover, the CD200-CD200r axis could have a previously unidentified role in the BMMSC mediated immunosuppression.
format	article
author	Mika Pietilä Siri Lehtonen Elina Tuovinen Kaarina Lähteenmäki Saara Laitinen Hannu-Ville Leskelä Antti Nätynki Juha Pesälä Katrina Nordström Petri Lehenkari
author_facet	Mika Pietilä Siri Lehtonen Elina Tuovinen Kaarina Lähteenmäki Saara Laitinen Hannu-Ville Leskelä Antti Nätynki Juha Pesälä Katrina Nordström Petri Lehenkari
author_sort	Mika Pietilä
title	CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
title_short	CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
title_full	CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
title_fullStr	CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
title_full_unstemmed	CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
title_sort	cd200 positive human mesenchymal stem cells suppress tnf-alpha secretion from cd200 receptor positive macrophage-like cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ad08aae4cac149c496e2f344c16298e0
work_keys_str_mv	AT mikapietila cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT sirilehtonen cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT elinatuovinen cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT kaarinalahteenmaki cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT saaralaitinen cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT hannuvilleleskela cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT anttinatynki cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT juhapesala cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT katrinanordstrom cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells AT petrilehenkari cd200positivehumanmesenchymalstemcellssuppresstnfalphasecretionfromcd200receptorpositivemacrophagelikecells
_version_	1718423430906249216

CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.

Ejemplares similares