Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium

Uranium and nitrate are common groundwater pollutants near in situ leach uranium mines. However, we still lack techniques that can simultaneously immobilize uranium and reduce nitrate using a single bacterial species. In this study, the potential of simultaneous uranium immobilization and nitrate re...

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Autores principales:	Rehemanjiang Wufuer, Jia Duo, Wenfeng Li, Jinglong Fan, Xiangliang Pan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Clostridium</i> sp. PXL2 immobilization reduction Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/ad09cd8e12e44fb686dcd6df201c4c68
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spelling	oai:doaj.org-article:ad09cd8e12e44fb686dcd6df201c4c682021-11-25T19:15:18ZBioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium10.3390/w132231882073-4441https://doaj.org/article/ad09cd8e12e44fb686dcd6df201c4c682021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/22/3188https://doaj.org/toc/2073-4441Uranium and nitrate are common groundwater pollutants near in situ leach uranium mines. However, we still lack techniques that can simultaneously immobilize uranium and reduce nitrate using a single bacterial species. In this study, the potential of simultaneous uranium immobilization and nitrate reduction by a single AFODN (anaerobic Fe(II) oxidizing denitrifier), <i>Clostridium</i> sp. PXL2, was investigated. <i>Clostridium</i> sp. PXL2 showed tolerance to U(VI) concentrations varying from 4.2 µM to 42 µM. The U(VI) immobilization and nitrate reduction rates in groundwater samples inoculated with this bacterium reached up to 75.1% and 55.7%, respectively, under neutral conditions. Exposure to oxidation conditions led to further U(VI) removal but did not show any noticeable effect on nitrate reduction. The U(VI) immobilization rate reached up to 85% with an increased Fe(II) initial concentration, but this inhibited nitrate reduction. SEM (scanning electron microscopy) coupled with EDS (energy dispersive spectroscopy) showed that the U(VI) immobilization was mainly due to sorption to amorphous ferric oxides. U(VI) and nitrate bioremediation by AFODNs, including <i>Clostridium</i> sp. PXL2, may provide a promising method for the treatment of uranium- and nitrate-contaminated groundwater after the in situ leach mining of uranium.Rehemanjiang WufuerJia DuoWenfeng LiJinglong FanXiangliang PanMDPI AGarticle<i>Clostridium</i> sp. PXL2immobilizationreductionHydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 3188, p 3188 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Clostridium</i> sp. PXL2 immobilization reduction Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	<i>Clostridium</i> sp. PXL2 immobilization reduction Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Rehemanjiang Wufuer Jia Duo Wenfeng Li Jinglong Fan Xiangliang Pan Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium
description	Uranium and nitrate are common groundwater pollutants near in situ leach uranium mines. However, we still lack techniques that can simultaneously immobilize uranium and reduce nitrate using a single bacterial species. In this study, the potential of simultaneous uranium immobilization and nitrate reduction by a single AFODN (anaerobic Fe(II) oxidizing denitrifier), <i>Clostridium</i> sp. PXL2, was investigated. <i>Clostridium</i> sp. PXL2 showed tolerance to U(VI) concentrations varying from 4.2 µM to 42 µM. The U(VI) immobilization and nitrate reduction rates in groundwater samples inoculated with this bacterium reached up to 75.1% and 55.7%, respectively, under neutral conditions. Exposure to oxidation conditions led to further U(VI) removal but did not show any noticeable effect on nitrate reduction. The U(VI) immobilization rate reached up to 85% with an increased Fe(II) initial concentration, but this inhibited nitrate reduction. SEM (scanning electron microscopy) coupled with EDS (energy dispersive spectroscopy) showed that the U(VI) immobilization was mainly due to sorption to amorphous ferric oxides. U(VI) and nitrate bioremediation by AFODNs, including <i>Clostridium</i> sp. PXL2, may provide a promising method for the treatment of uranium- and nitrate-contaminated groundwater after the in situ leach mining of uranium.
format	article
author	Rehemanjiang Wufuer Jia Duo Wenfeng Li Jinglong Fan Xiangliang Pan
author_facet	Rehemanjiang Wufuer Jia Duo Wenfeng Li Jinglong Fan Xiangliang Pan
author_sort	Rehemanjiang Wufuer
title	Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium
title_short	Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium
title_full	Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium
title_fullStr	Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium
title_full_unstemmed	Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium
title_sort	bioremediation of uranium- and nitrate-contaminated groundwater after the in situ leach mining of uranium
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ad09cd8e12e44fb686dcd6df201c4c68
work_keys_str_mv	AT rehemanjiangwufuer bioremediationofuraniumandnitratecontaminatedgroundwateraftertheinsituleachminingofuranium AT jiaduo bioremediationofuraniumandnitratecontaminatedgroundwateraftertheinsituleachminingofuranium AT wenfengli bioremediationofuraniumandnitratecontaminatedgroundwateraftertheinsituleachminingofuranium AT jinglongfan bioremediationofuraniumandnitratecontaminatedgroundwateraftertheinsituleachminingofuranium AT xiangliangpan bioremediationofuraniumandnitratecontaminatedgroundwateraftertheinsituleachminingofuranium
_version_	1718410115207397376

Bioremediation of Uranium- and Nitrate-Contaminated Groundwater after the In Situ Leach Mining of Uranium

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