Influence of magnetic fields on magneto-aerotaxis.

The response of cells to changes in their physico-chemical micro-environment is essential to their survival. For example, bacterial magnetotaxis uses the Earth's magnetic field together with chemical sensing to help microorganisms move towards favoured habitats. The studies of such complex resp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mathieu Bennet, Aongus McCarthy, Dmitri Fix, Matthew R Edwards, Felix Repp, Peter Vach, John W C Dunlop, Metin Sitti, Gerald S Buller, Stefan Klumpp, Damien Faivre
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad0cef4085f04a8c838cffb8a7da086f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad0cef4085f04a8c838cffb8a7da086f
record_format	dspace
spelling	oai:doaj.org-article:ad0cef4085f04a8c838cffb8a7da086f2021-11-25T06:10:08ZInfluence of magnetic fields on magneto-aerotaxis.1932-620310.1371/journal.pone.0101150https://doaj.org/article/ad0cef4085f04a8c838cffb8a7da086f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24983865/?tool=EBIhttps://doaj.org/toc/1932-6203The response of cells to changes in their physico-chemical micro-environment is essential to their survival. For example, bacterial magnetotaxis uses the Earth's magnetic field together with chemical sensing to help microorganisms move towards favoured habitats. The studies of such complex responses are lacking a method that permits the simultaneous mapping of the chemical environment and the response of the organisms, and the ability to generate a controlled physiological magnetic field. We have thus developed a multi-modal microscopy platform that fulfils these requirements. Using simultaneous fluorescence and high-speed imaging in conjunction with diffusion and aerotactic models, we characterized the magneto-aerotaxis of Magnetospirillum gryphiswaldense. We assessed the influence of the magnetic field (orientation; strength) on the formation and the dynamic of a micro-aerotactic band (size, dynamic, position). As previously described by models of magnetotaxis, the application of a magnetic field pointing towards the anoxic zone of an oxygen gradient results in an enhanced aerotaxis even down to Earth's magnetic field strength. We found that neither a ten-fold increase of the field strength nor a tilt of 45° resulted in a significant change of the aerotactic efficiency. However, when the field strength is zeroed or when the field angle is tilted to 90°, the magneto-aerotaxis efficiency is drastically reduced. The classical model of magneto-aerotaxis assumes a response proportional to the cosine of the angle difference between the directions of the oxygen gradient and that of the magnetic field. Our experimental evidence however shows that this behaviour is more complex than assumed in this model, thus opening up new avenues for research.Mathieu BennetAongus McCarthyDmitri FixMatthew R EdwardsFelix ReppPeter VachJohn W C DunlopMetin SittiGerald S BullerStefan KlumppDamien FaivrePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101150 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathieu Bennet Aongus McCarthy Dmitri Fix Matthew R Edwards Felix Repp Peter Vach John W C Dunlop Metin Sitti Gerald S Buller Stefan Klumpp Damien Faivre Influence of magnetic fields on magneto-aerotaxis.
description	The response of cells to changes in their physico-chemical micro-environment is essential to their survival. For example, bacterial magnetotaxis uses the Earth's magnetic field together with chemical sensing to help microorganisms move towards favoured habitats. The studies of such complex responses are lacking a method that permits the simultaneous mapping of the chemical environment and the response of the organisms, and the ability to generate a controlled physiological magnetic field. We have thus developed a multi-modal microscopy platform that fulfils these requirements. Using simultaneous fluorescence and high-speed imaging in conjunction with diffusion and aerotactic models, we characterized the magneto-aerotaxis of Magnetospirillum gryphiswaldense. We assessed the influence of the magnetic field (orientation; strength) on the formation and the dynamic of a micro-aerotactic band (size, dynamic, position). As previously described by models of magnetotaxis, the application of a magnetic field pointing towards the anoxic zone of an oxygen gradient results in an enhanced aerotaxis even down to Earth's magnetic field strength. We found that neither a ten-fold increase of the field strength nor a tilt of 45° resulted in a significant change of the aerotactic efficiency. However, when the field strength is zeroed or when the field angle is tilted to 90°, the magneto-aerotaxis efficiency is drastically reduced. The classical model of magneto-aerotaxis assumes a response proportional to the cosine of the angle difference between the directions of the oxygen gradient and that of the magnetic field. Our experimental evidence however shows that this behaviour is more complex than assumed in this model, thus opening up new avenues for research.
format	article
author	Mathieu Bennet Aongus McCarthy Dmitri Fix Matthew R Edwards Felix Repp Peter Vach John W C Dunlop Metin Sitti Gerald S Buller Stefan Klumpp Damien Faivre
author_facet	Mathieu Bennet Aongus McCarthy Dmitri Fix Matthew R Edwards Felix Repp Peter Vach John W C Dunlop Metin Sitti Gerald S Buller Stefan Klumpp Damien Faivre
author_sort	Mathieu Bennet
title	Influence of magnetic fields on magneto-aerotaxis.
title_short	Influence of magnetic fields on magneto-aerotaxis.
title_full	Influence of magnetic fields on magneto-aerotaxis.
title_fullStr	Influence of magnetic fields on magneto-aerotaxis.
title_full_unstemmed	Influence of magnetic fields on magneto-aerotaxis.
title_sort	influence of magnetic fields on magneto-aerotaxis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ad0cef4085f04a8c838cffb8a7da086f
work_keys_str_mv	AT mathieubennet influenceofmagneticfieldsonmagnetoaerotaxis AT aongusmccarthy influenceofmagneticfieldsonmagnetoaerotaxis AT dmitrifix influenceofmagneticfieldsonmagnetoaerotaxis AT matthewredwards influenceofmagneticfieldsonmagnetoaerotaxis AT felixrepp influenceofmagneticfieldsonmagnetoaerotaxis AT petervach influenceofmagneticfieldsonmagnetoaerotaxis AT johnwcdunlop influenceofmagneticfieldsonmagnetoaerotaxis AT metinsitti influenceofmagneticfieldsonmagnetoaerotaxis AT geraldsbuller influenceofmagneticfieldsonmagnetoaerotaxis AT stefanklumpp influenceofmagneticfieldsonmagnetoaerotaxis AT damienfaivre influenceofmagneticfieldsonmagnetoaerotaxis
_version_	1718414123491917824

Influence of magnetic fields on magneto-aerotaxis.

Ejemplares similares