Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units

INTRODUCTION: Gamma Irradiation of blood products accentuates biochemical changes in the blood stored at 4°C. This study tried to compare the changes in potassium, sodium, glucose, lactate, and lactate dehydrogenase (LDH) levels in packed red blood cell (PRBC) units irradiated at various time points...

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Autores principales:	Pramanya Balasubramanyam, Abhishekh Basavarajegowda, Nandeesha Hanumanthappa, Vir Singh Negi, K T Harichandrakumar
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	blood storage irradiation serial biochemistry storage lesions Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/ad14ac46161a48adae69b9b288bb88f7
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spelling	oai:doaj.org-article:ad14ac46161a48adae69b9b288bb88f72021-11-12T10:08:28ZIrradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units0973-62471998-356510.4103/ajts.ajts_71_21https://doaj.org/article/ad14ac46161a48adae69b9b288bb88f72021-01-01T00:00:00Zhttp://www.ajts.org/article.asp?issn=0973-6247;year=2021;volume=15;issue=2;spage=172;epage=178;aulast=Balasubramanyamhttps://doaj.org/toc/0973-6247https://doaj.org/toc/1998-3565INTRODUCTION: Gamma Irradiation of blood products accentuates biochemical changes in the blood stored at 4°C. This study tried to compare the changes in potassium, sodium, glucose, lactate, and lactate dehydrogenase (LDH) levels in packed red blood cell (PRBC) units irradiated at various time points and then stored versus those stored for a particular period then irradiated.METHODOLOGY: One hundred and eighty units of RBCs were randomly assigned equally to be irradiated or not. Eighteen units each were irradiated by gamma irradiator using cobalt 60 (BI 2000) on day 1, 7, 14, 21, and 28 of their storage, respectively, in the irradiation group. All the units were assessed for their plasma levels of potassium, sodium, glucose, LDH, and lactate by clinical chemistry auto analyzer Beckman coulter AU680 weekly. The values were documented and analyzed by SPSS.RESULTS: Baseline values on day 1 for studied biochemical parameters were comparable between irradiated and nonirradiated groups. Mean values of potassium, lactate and LDH were higher in irradiated than nonirradiated PRBC bags. In contrast, Sodium and Glucose mean values were lower than baseline values. Maximum cumulative mean values were noted in day-21 irradiated bags when the parameters were measured on day-28 for potassium and lactate levels. This was followed by day 14 irradiated bags, followed by day 7 irradiated bags.CONCLUSION: The study indicates that irradiation of red cells later in their storage period had comparatively more detrimental changes in relation to potassium and lactate than irradiation in earlier days. Consideration of irradiation to be performed as close to the issue as possible to reduce a lesser number of days of storage postirradiation is to be explored.Pramanya BalasubramanyamAbhishekh BasavarajegowdaNandeesha HanumanthappaVir Singh NegiK T HarichandrakumarWolters Kluwer Medknow Publicationsarticleblood storageirradiationserial biochemistrystorage lesionsDiseases of the blood and blood-forming organsRC633-647.5ENAsian Journal of Transfusion Science, Vol 15, Iss 2, Pp 172-178 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	blood storage irradiation serial biochemistry storage lesions Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	blood storage irradiation serial biochemistry storage lesions Diseases of the blood and blood-forming organs RC633-647.5 Pramanya Balasubramanyam Abhishekh Basavarajegowda Nandeesha Hanumanthappa Vir Singh Negi K T Harichandrakumar Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units
description	INTRODUCTION: Gamma Irradiation of blood products accentuates biochemical changes in the blood stored at 4°C. This study tried to compare the changes in potassium, sodium, glucose, lactate, and lactate dehydrogenase (LDH) levels in packed red blood cell (PRBC) units irradiated at various time points and then stored versus those stored for a particular period then irradiated.METHODOLOGY: One hundred and eighty units of RBCs were randomly assigned equally to be irradiated or not. Eighteen units each were irradiated by gamma irradiator using cobalt 60 (BI 2000) on day 1, 7, 14, 21, and 28 of their storage, respectively, in the irradiation group. All the units were assessed for their plasma levels of potassium, sodium, glucose, LDH, and lactate by clinical chemistry auto analyzer Beckman coulter AU680 weekly. The values were documented and analyzed by SPSS.RESULTS: Baseline values on day 1 for studied biochemical parameters were comparable between irradiated and nonirradiated groups. Mean values of potassium, lactate and LDH were higher in irradiated than nonirradiated PRBC bags. In contrast, Sodium and Glucose mean values were lower than baseline values. Maximum cumulative mean values were noted in day-21 irradiated bags when the parameters were measured on day-28 for potassium and lactate levels. This was followed by day 14 irradiated bags, followed by day 7 irradiated bags.CONCLUSION: The study indicates that irradiation of red cells later in their storage period had comparatively more detrimental changes in relation to potassium and lactate than irradiation in earlier days. Consideration of irradiation to be performed as close to the issue as possible to reduce a lesser number of days of storage postirradiation is to be explored.
format	article
author	Pramanya Balasubramanyam Abhishekh Basavarajegowda Nandeesha Hanumanthappa Vir Singh Negi K T Harichandrakumar
author_facet	Pramanya Balasubramanyam Abhishekh Basavarajegowda Nandeesha Hanumanthappa Vir Singh Negi K T Harichandrakumar
author_sort	Pramanya Balasubramanyam
title	Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units
title_short	Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units
title_full	Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units
title_fullStr	Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units
title_full_unstemmed	Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units
title_sort	irradiating stored blood and storing irradiated blood: is it different? - a study of serial changes in biochemical parameters of red blood cell units
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/ad14ac46161a48adae69b9b288bb88f7
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Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units

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