DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY

Abstract. The present work was aimed to evaluation of phenotype and functional properties of IFNα-induced dendritic cells (DCs) in normal pregnancy, and in cases complicated with suprarenal hyperandrogenia (HA), as well as in vitro assessment of dehydroepiandrosterone sulfate (DHEAS) effects upon DC...

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Autores principales:	E. R. Chernykh, N. V. Seledtsova, O. Yu. Leplina, M. A. Tikhonova, T. V. Tyrinova, E. V. Kurganova, N. A. Khonina, A. A. Ostanin, N. M. Pasman
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	dendritic cells phenotype th1/th2 dehydroepiandrosterone sulfate pregnancy Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ad1a23389bc24e1a8ee43a9149605742
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spelling	oai:doaj.org-article:ad1a23389bc24e1a8ee43a91496057422021-11-18T08:03:40ZDENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY1563-06252313-741X10.15789/1563-0625-2009-6-541-548https://doaj.org/article/ad1a23389bc24e1a8ee43a91496057422014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/365https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The present work was aimed to evaluation of phenotype and functional properties of IFNα-induced dendritic cells (DCs) in normal pregnancy, and in cases complicated with suprarenal hyperandrogenia (HA), as well as in vitro assessment of dehydroepiandrosterone sulfate (DHEAS) effects upon DC functions. As compared with non-pregnant women, DCs from healthy pregnant women are notable for impaired maturation/activation, whereas fractions of mature (CD83+) and activated (CD25+) cells DC were similar in normal pregnancy and in women with HA. Blood sera from healthy pregnant women inhibited generation of mature DCs, whereas sera of women with HA and direct supplementation with DHEAS enhanced maturation and activation of DCs. Functional analysis of DC capacity to stimulate Th1 (IFNγ) and Th2 (IL-4) cytokine expression in MLC has shown that, in contrast to non-pregnant women with T1-cell activation by DCs, the DCs from healthy pregnant women caused predominant activation of CD3+IL-4+Т-cells. In HA-complicated pregnancy, DCs were capable to stimulate both Th1- and Th2-cytokine production in T-cell populations. Meanwhile, addition of DHEAS to DCs cultures were accompanied by enhancement of their T1- polarizing activity. DCs from women with normal pregnancy exerted inhibitory effect upon activated NK-cells that was evidenced by a relative decrease in CD56+CD16+ cells counts and increased apoptosis levels. This function of DCs was partially abrogated in presence of DHEAS. The results obtained reveal new mechanisms of immune/ endocrine nteractions in normal and complicated pregnancy.E. R. ChernykhN. V. SeledtsovaO. Yu. LeplinaM. A. TikhonovaT. V. TyrinovaE. V. KurganovaN. A. KhoninaA. A. OstaninN. M. PasmanSPb RAACIarticledendritic cellsphenotypeth1/th2dehydroepiandrosterone sulfatepregnancyImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 6, Pp 541-548 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	dendritic cells phenotype th1/th2 dehydroepiandrosterone sulfate pregnancy Immunologic diseases. Allergy RC581-607
spellingShingle	dendritic cells phenotype th1/th2 dehydroepiandrosterone sulfate pregnancy Immunologic diseases. Allergy RC581-607 E. R. Chernykh N. V. Seledtsova O. Yu. Leplina M. A. Tikhonova T. V. Tyrinova E. V. Kurganova N. A. Khonina A. A. Ostanin N. M. Pasman DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY
description	Abstract. The present work was aimed to evaluation of phenotype and functional properties of IFNα-induced dendritic cells (DCs) in normal pregnancy, and in cases complicated with suprarenal hyperandrogenia (HA), as well as in vitro assessment of dehydroepiandrosterone sulfate (DHEAS) effects upon DC functions. As compared with non-pregnant women, DCs from healthy pregnant women are notable for impaired maturation/activation, whereas fractions of mature (CD83+) and activated (CD25+) cells DC were similar in normal pregnancy and in women with HA. Blood sera from healthy pregnant women inhibited generation of mature DCs, whereas sera of women with HA and direct supplementation with DHEAS enhanced maturation and activation of DCs. Functional analysis of DC capacity to stimulate Th1 (IFNγ) and Th2 (IL-4) cytokine expression in MLC has shown that, in contrast to non-pregnant women with T1-cell activation by DCs, the DCs from healthy pregnant women caused predominant activation of CD3+IL-4+Т-cells. In HA-complicated pregnancy, DCs were capable to stimulate both Th1- and Th2-cytokine production in T-cell populations. Meanwhile, addition of DHEAS to DCs cultures were accompanied by enhancement of their T1- polarizing activity. DCs from women with normal pregnancy exerted inhibitory effect upon activated NK-cells that was evidenced by a relative decrease in CD56+CD16+ cells counts and increased apoptosis levels. This function of DCs was partially abrogated in presence of DHEAS. The results obtained reveal new mechanisms of immune/ endocrine nteractions in normal and complicated pregnancy.
format	article
author	E. R. Chernykh N. V. Seledtsova O. Yu. Leplina M. A. Tikhonova T. V. Tyrinova E. V. Kurganova N. A. Khonina A. A. Ostanin N. M. Pasman
author_facet	E. R. Chernykh N. V. Seledtsova O. Yu. Leplina M. A. Tikhonova T. V. Tyrinova E. V. Kurganova N. A. Khonina A. A. Ostanin N. M. Pasman
author_sort	E. R. Chernykh
title	DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY
title_short	DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY
title_full	DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY
title_fullStr	DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY
title_full_unstemmed	DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY
title_sort	dendritic cells as possible regulators of immune re-organization in pregnancy
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/ad1a23389bc24e1a8ee43a9149605742
work_keys_str_mv	AT erchernykh dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT nvseledtsova dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT oyuleplina dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT matikhonova dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT tvtyrinova dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT evkurganova dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT nakhonina dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT aaostanin dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy AT nmpasman dendriticcellsaspossibleregulatorsofimmunereorganizationinpregnancy
_version_	1718422498746302464

DENDRITIC CELLS AS POSSIBLE REGULATORS OF IMMUNE RE-ORGANIZATION IN PREGNANCY

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