Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes

Susann Grosse,1 Jørgen Stenvik,1,2 Asbjørn M Nilsen1 1Department of Cancer Research and Molecular Medicine, 2Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway Abstract: Co-stimulation of the immune system to more than...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Grosse S, Stenvik J, Nilsen AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Iron oxide nanoparticles primary human monocytes lipopolysaccharide Toll-like receptors Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ad1be379fee2400a8dcc70fb1e894932
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad1be379fee2400a8dcc70fb1e894932
record_format	dspace
spelling	oai:doaj.org-article:ad1be379fee2400a8dcc70fb1e8949322021-12-02T02:42:30ZIron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes1178-2013https://doaj.org/article/ad1be379fee2400a8dcc70fb1e8949322016-09-01T00:00:00Zhttps://www.dovepress.com/iron-oxide-nanoparticles-modulate-lipopolysaccharide-induced-inflammat-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Susann Grosse,1 Jørgen Stenvik,1,2 Asbjørn M Nilsen1 1Department of Cancer Research and Molecular Medicine, 2Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway Abstract: Co-stimulation of the immune system to more than one agent concomitantly is very common in real life, and considering the increasing use of engineered nanoparticles and nanomaterials, it is highly relevant to assess the ability of these materials to modulate key innate immune responses, which has not yet been studied in detail. We investigated the immunomodulatory effects of 10 nm and 30 nm iron oxide nanoparticles (IONPs) on primary human monocytes in the presence and absence of Toll-like receptor 4 agonist lipopolysaccharide (LPS). Prior to the cell studies, we characterized the physicochemical properties of the nanoparticles in cell culture medium and ensured that the nanoparticles were free from biological contamination. Cellular uptake of the IONPs in monocytes was assessed using transmission electron microscopy. Using enzyme-linked immunosorbent assay, we found that the IONPs per se did not induce the production of proinflammatory cytokines tumor necrosis factor-α, interleukin-6, and interleukin-1β. However, the IONPs had the ability to suppress LPS-induced nuclear factor kappa B activation and production of proinflammatory cytokines in primary human monocytes in an LPS and a particle dose-dependent manner. Using confocal microscopy and fluorescently labeled LPS, we showed that the effects correlated with impaired LPS internalization by monocytes in the presence of IONPs, which could be partly explained by LPS adsorption onto the nanoparticle surface. Additionally, the results from particle pretreatment experiments indicate that other cellular mechanisms might also play a role in the observed effects, which warrants further studies to elucidate the additional mechanisms underlying the capacity of IONPs to alter the reactivity of monocytes to LPS and to mount an appropriate cellular response. Keywords: iron oxide nanoparticles, primary human monocytes, PBMCs, cytokines, LPS adsorption, Toll-like receptorsGrosse SStenvik JNilsen AMDove Medical PressarticleIron oxide nanoparticlesprimary human monocyteslipopolysaccharideToll-like receptorsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4625-4642 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Iron oxide nanoparticles primary human monocytes lipopolysaccharide Toll-like receptors Medicine (General) R5-920
spellingShingle	Iron oxide nanoparticles primary human monocytes lipopolysaccharide Toll-like receptors Medicine (General) R5-920 Grosse S Stenvik J Nilsen AM Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
description	Susann Grosse,1 Jørgen Stenvik,1,2 Asbjørn M Nilsen1 1Department of Cancer Research and Molecular Medicine, 2Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway Abstract: Co-stimulation of the immune system to more than one agent concomitantly is very common in real life, and considering the increasing use of engineered nanoparticles and nanomaterials, it is highly relevant to assess the ability of these materials to modulate key innate immune responses, which has not yet been studied in detail. We investigated the immunomodulatory effects of 10 nm and 30 nm iron oxide nanoparticles (IONPs) on primary human monocytes in the presence and absence of Toll-like receptor 4 agonist lipopolysaccharide (LPS). Prior to the cell studies, we characterized the physicochemical properties of the nanoparticles in cell culture medium and ensured that the nanoparticles were free from biological contamination. Cellular uptake of the IONPs in monocytes was assessed using transmission electron microscopy. Using enzyme-linked immunosorbent assay, we found that the IONPs per se did not induce the production of proinflammatory cytokines tumor necrosis factor-α, interleukin-6, and interleukin-1β. However, the IONPs had the ability to suppress LPS-induced nuclear factor kappa B activation and production of proinflammatory cytokines in primary human monocytes in an LPS and a particle dose-dependent manner. Using confocal microscopy and fluorescently labeled LPS, we showed that the effects correlated with impaired LPS internalization by monocytes in the presence of IONPs, which could be partly explained by LPS adsorption onto the nanoparticle surface. Additionally, the results from particle pretreatment experiments indicate that other cellular mechanisms might also play a role in the observed effects, which warrants further studies to elucidate the additional mechanisms underlying the capacity of IONPs to alter the reactivity of monocytes to LPS and to mount an appropriate cellular response. Keywords: iron oxide nanoparticles, primary human monocytes, PBMCs, cytokines, LPS adsorption, Toll-like receptors
format	article
author	Grosse S Stenvik J Nilsen AM
author_facet	Grosse S Stenvik J Nilsen AM
author_sort	Grosse S
title	Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
title_short	Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
title_full	Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
title_fullStr	Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
title_full_unstemmed	Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
title_sort	iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/ad1be379fee2400a8dcc70fb1e894932
work_keys_str_mv	AT grosses ironoxidenanoparticlesmodulatelipopolysaccharideinducedinflammatoryresponsesinprimaryhumanmonocytes AT stenvikj ironoxidenanoparticlesmodulatelipopolysaccharideinducedinflammatoryresponsesinprimaryhumanmonocytes AT nilsenam ironoxidenanoparticlesmodulatelipopolysaccharideinducedinflammatoryresponsesinprimaryhumanmonocytes
_version_	1718402244192239616

Iron oxide nanoparticles modulate lipopolysaccharide-induced inflammatory responses in primary human monocytes

Ejemplares similares