Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers

Abstract Despite the highly economic value of tea in Africa, its genetic and geographic origins remain largely unexplored. Here we address this by collecting 439 samples across 11 countries in Africa and Asia to investigate the origin and genepool composition of African tea based on 23 nuclear micro...

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Autores principales:	Moses Cheloti Wambulwa, Muditha Kasun Meegahakumbura, Samson Kamunya, Alice Muchugi, Michael Möller, Jie Liu, Jian-Chu Xu, De-Zhu Li, Lian-Ming Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad1c11cff5d041d7b80d0d15f3e942a7
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spelling	oai:doaj.org-article:ad1c11cff5d041d7b80d0d15f3e942a72021-12-02T16:06:21ZMultiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers10.1038/s41598-017-04228-02045-2322https://doaj.org/article/ad1c11cff5d041d7b80d0d15f3e942a72017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04228-0https://doaj.org/toc/2045-2322Abstract Despite the highly economic value of tea in Africa, its genetic and geographic origins remain largely unexplored. Here we address this by collecting 439 samples across 11 countries in Africa and Asia to investigate the origin and genepool composition of African tea based on 23 nuclear microsatellites loci (nSSRs) and three cpDNA intergenic spacer regions. Our results indicated that the African tea represents a potpourri originating from multiple introductions over time. The nSSR analysis revealed that the majority (79%) of tea accessions collected in Africa belong to Indian Assam tea which have likely originated from India and/or Sri Lanka. The patterns of nSSR variation also showed that Chinese Assam tea is genetically distinct from Indian Assam tea, and has rarely been used in African tea breeding efforts since only 4% of the African tea accessions possessed this genotype. We found a total of 22 cpDNA haplotypes, which grouped into three main geographic clades that were concordant with the distribution of microsatellite genotypes. Several private cpDNA haplotypes were identified in Chinese Assam tea in Southern Yunnan province of China. Therefore Chinese Assam tea will be important for the enrichment of African tea gene pools. Our results is a useful guide in future tea breeding programmes in Africa.Moses Cheloti WambulwaMuditha Kasun MeegahakumburaSamson KamunyaAlice MuchugiMichael MöllerJie LiuJian-Chu XuDe-Zhu LiLian-Ming GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moses Cheloti Wambulwa Muditha Kasun Meegahakumbura Samson Kamunya Alice Muchugi Michael Möller Jie Liu Jian-Chu Xu De-Zhu Li Lian-Ming Gao Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers
description	Abstract Despite the highly economic value of tea in Africa, its genetic and geographic origins remain largely unexplored. Here we address this by collecting 439 samples across 11 countries in Africa and Asia to investigate the origin and genepool composition of African tea based on 23 nuclear microsatellites loci (nSSRs) and three cpDNA intergenic spacer regions. Our results indicated that the African tea represents a potpourri originating from multiple introductions over time. The nSSR analysis revealed that the majority (79%) of tea accessions collected in Africa belong to Indian Assam tea which have likely originated from India and/or Sri Lanka. The patterns of nSSR variation also showed that Chinese Assam tea is genetically distinct from Indian Assam tea, and has rarely been used in African tea breeding efforts since only 4% of the African tea accessions possessed this genotype. We found a total of 22 cpDNA haplotypes, which grouped into three main geographic clades that were concordant with the distribution of microsatellite genotypes. Several private cpDNA haplotypes were identified in Chinese Assam tea in Southern Yunnan province of China. Therefore Chinese Assam tea will be important for the enrichment of African tea gene pools. Our results is a useful guide in future tea breeding programmes in Africa.
format	article
author	Moses Cheloti Wambulwa Muditha Kasun Meegahakumbura Samson Kamunya Alice Muchugi Michael Möller Jie Liu Jian-Chu Xu De-Zhu Li Lian-Ming Gao
author_facet	Moses Cheloti Wambulwa Muditha Kasun Meegahakumbura Samson Kamunya Alice Muchugi Michael Möller Jie Liu Jian-Chu Xu De-Zhu Li Lian-Ming Gao
author_sort	Moses Cheloti Wambulwa
title	Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers
title_short	Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers
title_full	Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers
title_fullStr	Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers
title_full_unstemmed	Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers
title_sort	multiple origins and a narrow genepool characterise the african tea germplasm: concordant patterns revealed by nuclear and plastid dna markers
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ad1c11cff5d041d7b80d0d15f3e942a7
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Multiple origins and a narrow genepool characterise the African tea germplasm: concordant patterns revealed by nuclear and plastid DNA markers

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