S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia

Hager Bourog&acirc;a, Imen Hellal, Jihene Hassen, Imen Fathallah, Abdeljelil GhramLaboratoire d&#39;Epid&eacute;miologie et de Microbiologie V&eacute;t&eacute;rinaire, Institut Pasteur de Tunis, Tunis, TunisiaPurpose: Tissue samples were collected from suspect...

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Autores principales:	Ghram A, Fathallah I, Hassen J, Hellal I, Bourog&acirc;a H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/ad1dbf2755754a8fab099ec247763a89
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spelling	oai:doaj.org-article:ad1dbf2755754a8fab099ec247763a892021-12-02T04:58:45ZS1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia2230-2034https://doaj.org/article/ad1dbf2755754a8fab099ec247763a892012-07-01T00:00:00Zhttp://www.dovepress.com/s1-gene-sequence-analysis-of-new-variant-isolates-of-avian-infectious--a10397https://doaj.org/toc/2230-2034Hager Bourog&acirc;a, Imen Hellal, Jihene Hassen, Imen Fathallah, Abdeljelil GhramLaboratoire d&#39;Epid&eacute;miologie et de Microbiologie V&eacute;t&eacute;rinaire, Institut Pasteur de Tunis, Tunis, TunisiaPurpose: Tissue samples were collected from suspected broiler flocks showing respiratory signs to identify infectious bronchitis virus (IBV), characterize emerging field strains, and study their relationships with the Massachusetts H120 strain, the only IB vaccine used in Tunisia.Samples and methods: Several IBV isolates were identified from field samples collected from flocks located in different regions in the northeast of Tunisia. The IBV isolates were characterized and compared to commonly used vaccine strains (including 793B, D274, and H120 types), other reference IBV strains from Europe, and the recently characterized Tunisian field variants TN20/00, TN200/01, and TN335/01. Reverse transcription&ndash;polymerase chain reaction and nucleotide sequencing analyses of the hypervariable regions of the S1 gene were carried out.Results: Four new IBV variants were isolated during the period 2007&ndash;10 and were designated TN295/07, TN296/07, TN556/07, and TN557/07. The amino acid sequence data showed 100% similarity between TN295/07 and TN296/07, suggesting that these two isolates are identical and belong to the same genotype. Similar results were demonstrated for TN556/07 and TN557/07. Sequence identity values indicated that TN296/07 and TN556/07 share 55% amino acid homologies between each other, but are very different from the reference IBV serotypes, in particular the H120 strain. It was also shown that they have 50%&ndash;77% similarities with the Tunisian virus isolated between 2000 and 2001. Phylogenetic clustering allowed classification of these Tunisian isolates as new genotypes that are closer to TN200/01, TN335/01 Tunisian field variants, and Italy02 variant than MassH120 vaccine strain.Conclusion: S1 sequence analyses confirmed the cocirculation of H120 vaccine strain with novel IBV variants isolated from Tunisian field.Keywords: IBV, new genotypes, RT-PCR, S1 gene sequencing, phylogeny analysis, TunisiaGhram AFathallah IHassen JHellal IBourog&acirc;a HDove Medical PressarticleVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol 2012, Iss default, Pp 41-48 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Veterinary medicine SF600-1100
spellingShingle	Veterinary medicine SF600-1100 Ghram A Fathallah I Hassen J Hellal I Bourog&acirc;a H S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia
description	Hager Bourog&acirc;a, Imen Hellal, Jihene Hassen, Imen Fathallah, Abdeljelil GhramLaboratoire d&#39;Epid&eacute;miologie et de Microbiologie V&eacute;t&eacute;rinaire, Institut Pasteur de Tunis, Tunis, TunisiaPurpose: Tissue samples were collected from suspected broiler flocks showing respiratory signs to identify infectious bronchitis virus (IBV), characterize emerging field strains, and study their relationships with the Massachusetts H120 strain, the only IB vaccine used in Tunisia.Samples and methods: Several IBV isolates were identified from field samples collected from flocks located in different regions in the northeast of Tunisia. The IBV isolates were characterized and compared to commonly used vaccine strains (including 793B, D274, and H120 types), other reference IBV strains from Europe, and the recently characterized Tunisian field variants TN20/00, TN200/01, and TN335/01. Reverse transcription&ndash;polymerase chain reaction and nucleotide sequencing analyses of the hypervariable regions of the S1 gene were carried out.Results: Four new IBV variants were isolated during the period 2007&ndash;10 and were designated TN295/07, TN296/07, TN556/07, and TN557/07. The amino acid sequence data showed 100% similarity between TN295/07 and TN296/07, suggesting that these two isolates are identical and belong to the same genotype. Similar results were demonstrated for TN556/07 and TN557/07. Sequence identity values indicated that TN296/07 and TN556/07 share 55% amino acid homologies between each other, but are very different from the reference IBV serotypes, in particular the H120 strain. It was also shown that they have 50%&ndash;77% similarities with the Tunisian virus isolated between 2000 and 2001. Phylogenetic clustering allowed classification of these Tunisian isolates as new genotypes that are closer to TN200/01, TN335/01 Tunisian field variants, and Italy02 variant than MassH120 vaccine strain.Conclusion: S1 sequence analyses confirmed the cocirculation of H120 vaccine strain with novel IBV variants isolated from Tunisian field.Keywords: IBV, new genotypes, RT-PCR, S1 gene sequencing, phylogeny analysis, Tunisia
format	article
author	Ghram A Fathallah I Hassen J Hellal I Bourog&acirc;a H
author_facet	Ghram A Fathallah I Hassen J Hellal I Bourog&acirc;a H
author_sort	Ghram A
title	S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia
title_short	S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia
title_full	S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia
title_fullStr	S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia
title_full_unstemmed	S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia
title_sort	s1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in tunisia
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ad1dbf2755754a8fab099ec247763a89
work_keys_str_mv	AT ghrama s1genesequenceanalysisofnewvariantisolatesofavianinfectiousbronchitisvirusintunisia AT fathallahi s1genesequenceanalysisofnewvariantisolatesofavianinfectiousbronchitisvirusintunisia AT hassenj s1genesequenceanalysisofnewvariantisolatesofavianinfectiousbronchitisvirusintunisia AT hellali s1genesequenceanalysisofnewvariantisolatesofavianinfectiousbronchitisvirusintunisia AT bourogampacircah s1genesequenceanalysisofnewvariantisolatesofavianinfectiousbronchitisvirusintunisia
_version_	1718400971357290496

S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia

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