Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo

Background. Belonging to the family of Combretaceae, the roots of Anogeissus leiocarpus are traditionally used to treat diabetes, wounds, infections, pain, and gastrointestinal diseases. To our knowledge, no genotoxicity assessment of the plant was reported. Hence, this study was designed to evaluat...

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Autores principales:	Aku Enam Motto, Povi Lawson-Evi, Aboudoulatif Diallo, Kwashie Eklu-Gadegbeku
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Toxicology. Poisons RA1190-1270
Acceso en línea:	https://doaj.org/article/ad25ad7149a043b2b37c5e637995d8c6
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spelling	oai:doaj.org-article:ad25ad7149a043b2b37c5e637995d8c62021-11-22T01:11:06ZGenotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo1687-820510.1155/2021/8020240https://doaj.org/article/ad25ad7149a043b2b37c5e637995d8c62021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8020240https://doaj.org/toc/1687-8205Background. Belonging to the family of Combretaceae, the roots of Anogeissus leiocarpus are traditionally used to treat diabetes, wounds, infections, pain, and gastrointestinal diseases. To our knowledge, no genotoxicity assessment of the plant was reported. Hence, this study was designed to evaluate the potential genotoxic and protective effects of extract of Anogeissus leiocarpus roots using the micronucleus test on mice bone marrow cells in vivo. Methods. Three different concentrations (250, 500, and 1000 mg·kg−1) of hydroalcoholic extract of roots of A. leiocarpus were administered daily for 7 days per os to mice, and the genotoxicity was induced by the administration ip of cyclophosphamide. Genotoxicity and cytotoxicity were evaluated by counting, respectively, the number of micronucleated polychromatic erythrocytes and polychromatic erythrocytes to total erythrocytes in the bone marrow of mice. Results. The administration of A. leiocarpus did neither increase the ratio of the polychromatic erythrocyte (PCE) nor the frequency of micronucleated PCE (MNPCE) significantly in the bone marrow cells of the mice, compared to the vehicle control animals. However, a significant increase in the incidence of MNPCE in the bone marrow cell of the cyclophosphamide-treated mice was found. Moreover, in the groups treated with the total extract of A. leiocarpus at different doses plus cyclophosphamide, there was a significant decrease p<0.0001 in MNPCEs compared to the positive controls, in a dose-dependent manner. Conclusion. This first finding reports that the extract of A. leiocarpus was neither genotoxic nor cytotoxic. However, it shows a protective effect against the genotoxicity and cytotoxicity induced by cyclophosphamide.Aku Enam MottoPovi Lawson-EviAboudoulatif DialloKwashie Eklu-GadegbekuHindawi LimitedarticleToxicology. PoisonsRA1190-1270ENJournal of Toxicology, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Toxicology. Poisons RA1190-1270
spellingShingle	Toxicology. Poisons RA1190-1270 Aku Enam Motto Povi Lawson-Evi Aboudoulatif Diallo Kwashie Eklu-Gadegbeku Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo
description	Background. Belonging to the family of Combretaceae, the roots of Anogeissus leiocarpus are traditionally used to treat diabetes, wounds, infections, pain, and gastrointestinal diseases. To our knowledge, no genotoxicity assessment of the plant was reported. Hence, this study was designed to evaluate the potential genotoxic and protective effects of extract of Anogeissus leiocarpus roots using the micronucleus test on mice bone marrow cells in vivo. Methods. Three different concentrations (250, 500, and 1000 mg·kg−1) of hydroalcoholic extract of roots of A. leiocarpus were administered daily for 7 days per os to mice, and the genotoxicity was induced by the administration ip of cyclophosphamide. Genotoxicity and cytotoxicity were evaluated by counting, respectively, the number of micronucleated polychromatic erythrocytes and polychromatic erythrocytes to total erythrocytes in the bone marrow of mice. Results. The administration of A. leiocarpus did neither increase the ratio of the polychromatic erythrocyte (PCE) nor the frequency of micronucleated PCE (MNPCE) significantly in the bone marrow cells of the mice, compared to the vehicle control animals. However, a significant increase in the incidence of MNPCE in the bone marrow cell of the cyclophosphamide-treated mice was found. Moreover, in the groups treated with the total extract of A. leiocarpus at different doses plus cyclophosphamide, there was a significant decrease p<0.0001 in MNPCEs compared to the positive controls, in a dose-dependent manner. Conclusion. This first finding reports that the extract of A. leiocarpus was neither genotoxic nor cytotoxic. However, it shows a protective effect against the genotoxicity and cytotoxicity induced by cyclophosphamide.
format	article
author	Aku Enam Motto Povi Lawson-Evi Aboudoulatif Diallo Kwashie Eklu-Gadegbeku
author_facet	Aku Enam Motto Povi Lawson-Evi Aboudoulatif Diallo Kwashie Eklu-Gadegbeku
author_sort	Aku Enam Motto
title	Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo
title_short	Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo
title_full	Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo
title_fullStr	Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo
title_full_unstemmed	Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo
title_sort	genotoxicity assessment and protective effect of anogeissus leiocarpus roots against cyclophosphamide-induced dna damage in vivo
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/ad25ad7149a043b2b37c5e637995d8c6
work_keys_str_mv	AT akuenammotto genotoxicityassessmentandprotectiveeffectofanogeissusleiocarpusrootsagainstcyclophosphamideinduceddnadamageinvivo AT povilawsonevi genotoxicityassessmentandprotectiveeffectofanogeissusleiocarpusrootsagainstcyclophosphamideinduceddnadamageinvivo AT aboudoulatifdiallo genotoxicityassessmentandprotectiveeffectofanogeissusleiocarpusrootsagainstcyclophosphamideinduceddnadamageinvivo AT kwashieeklugadegbeku genotoxicityassessmentandprotectiveeffectofanogeissusleiocarpusrootsagainstcyclophosphamideinduceddnadamageinvivo
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Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo

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