A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials

Abstract There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in s...

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Autores principales:	Siemon De Nys, Eveline Putzeys, Philippe Vervliet, Adrian Covaci, Imke Boonen, Marc Elskens, Jeroen Vanoirbeek, Lode Godderis, Bart Van Meerbeek, Kirsten L. Van Landuyt, Radu Corneliu Duca
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad2d8c5e531c46fcbb9eb7483967fe27
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spelling	oai:doaj.org-article:ad2d8c5e531c46fcbb9eb7483967fe272021-12-02T15:08:25ZA novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials10.1038/s41598-018-24815-z2045-2322https://doaj.org/article/ad2d8c5e531c46fcbb9eb7483967fe272018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24815-zhttps://doaj.org/toc/2045-2322Abstract There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), using an extraction step and additional derivatization of BPA with pyridine-3-sulfonyl chloride. Light-cured resin-based composites were incubated at 37 °C in 1 mL artificial saliva, which was refreshed daily for one week. The final protocol allows accurate quantification of very low levels of BPA in samples of artificial saliva (i.e. 1.10 pmol BPA/mL or 250 pg/mL). The daily BPA-release from dental composites, ranging from 1.10 to 7.46 pmol BPA/mL, was characterized over a period of 7 days. The highest total amount of BPA was released from Solitaire 2 (24.72 ± 2.86 pmol), followed by G-ænial Posterior (15.51 ± 0.88 pmol) and Filtek Supreme XTE (12.00 ± 1.31 pmol). In contrast, only trace amounts of BPA were released from Ceram.x Universal. This UPLC-MS/MS method might be used for clinical research focusing on the evaluation of the clinical relevance of BPA release from dental materials.Siemon De NysEveline PutzeysPhilippe VervlietAdrian CovaciImke BoonenMarc ElskensJeroen VanoirbeekLode GodderisBart Van MeerbeekKirsten L. Van LanduytRadu Corneliu DucaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siemon De Nys Eveline Putzeys Philippe Vervliet Adrian Covaci Imke Boonen Marc Elskens Jeroen Vanoirbeek Lode Godderis Bart Van Meerbeek Kirsten L. Van Landuyt Radu Corneliu Duca A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
description	Abstract There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), using an extraction step and additional derivatization of BPA with pyridine-3-sulfonyl chloride. Light-cured resin-based composites were incubated at 37 °C in 1 mL artificial saliva, which was refreshed daily for one week. The final protocol allows accurate quantification of very low levels of BPA in samples of artificial saliva (i.e. 1.10 pmol BPA/mL or 250 pg/mL). The daily BPA-release from dental composites, ranging from 1.10 to 7.46 pmol BPA/mL, was characterized over a period of 7 days. The highest total amount of BPA was released from Solitaire 2 (24.72 ± 2.86 pmol), followed by G-ænial Posterior (15.51 ± 0.88 pmol) and Filtek Supreme XTE (12.00 ± 1.31 pmol). In contrast, only trace amounts of BPA were released from Ceram.x Universal. This UPLC-MS/MS method might be used for clinical research focusing on the evaluation of the clinical relevance of BPA release from dental materials.
format	article
author	Siemon De Nys Eveline Putzeys Philippe Vervliet Adrian Covaci Imke Boonen Marc Elskens Jeroen Vanoirbeek Lode Godderis Bart Van Meerbeek Kirsten L. Van Landuyt Radu Corneliu Duca
author_facet	Siemon De Nys Eveline Putzeys Philippe Vervliet Adrian Covaci Imke Boonen Marc Elskens Jeroen Vanoirbeek Lode Godderis Bart Van Meerbeek Kirsten L. Van Landuyt Radu Corneliu Duca
author_sort	Siemon De Nys
title	A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
title_short	A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
title_full	A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
title_fullStr	A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
title_full_unstemmed	A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
title_sort	novel high sensitivity uplc-ms/ms method for the evaluation of bisphenol a leaching from dental materials
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ad2d8c5e531c46fcbb9eb7483967fe27
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A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials

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