Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo

Abstract Influenza viruses cause significant morbidity and mortality worldwide. Long-term or frequent use of approved anti-influenza agents has resulted in drug-resistant strains, thereby necessitating the discovery of new drugs. In this study, we found aprotinin, a serine protease inhibitor, as an...

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Autores principales:	Eun-Jung Song, Erica Españo, Sang-Mu Shim, Jeong-Hyun Nam, Jiyeon Kim, Kiho Lee, Song-Kyu Park, Chong-Kil Lee, Jeong-Ki Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad383a25f37f466e93110e0f247e0848
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spelling	oai:doaj.org-article:ad383a25f37f466e93110e0f247e08482021-12-02T14:29:15ZInhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo10.1038/s41598-021-88886-12045-2322https://doaj.org/article/ad383a25f37f466e93110e0f247e08482021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88886-1https://doaj.org/toc/2045-2322Abstract Influenza viruses cause significant morbidity and mortality worldwide. Long-term or frequent use of approved anti-influenza agents has resulted in drug-resistant strains, thereby necessitating the discovery of new drugs. In this study, we found aprotinin, a serine protease inhibitor, as an anti-influenza candidate through screening of compound libraries. Aprotinin has been previously reported to show inhibitory effects on a few influenza A virus (IAV) subtypes (e.g., seasonal H1N1 and H3N2). However, because there were no reports of its inhibitory effects on the other types of influenza viruses, we investigated the inhibitory effects of aprotinin in vitro on a wide range of influenza viruses, including avian and oseltamivir-resistant influenza virus strains. Our cell-based assay showed that aprotinin had inhibitory effects on seasonal human IAVs (H1N1 and H3N2 subtypes), avian IAVs (H5N2, H6N5, and H9N2 subtypes), an oseltamivir-resistant IAV, and a currently circulating influenza B virus. We have also confirmed its activity in mice infected with a lethal dose of influenza virus, showing a significant increase in survival rate. Our findings suggest that aprotinin has the capacity to inhibit a wide range of influenza virus subtypes and should be considered for development as a therapeutic agent against influenza.Eun-Jung SongErica EspañoSang-Mu ShimJeong-Hyun NamJiyeon KimKiho LeeSong-Kyu ParkChong-Kil LeeJeong-Ki KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eun-Jung Song Erica Españo Sang-Mu Shim Jeong-Hyun Nam Jiyeon Kim Kiho Lee Song-Kyu Park Chong-Kil Lee Jeong-Ki Kim Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo
description	Abstract Influenza viruses cause significant morbidity and mortality worldwide. Long-term or frequent use of approved anti-influenza agents has resulted in drug-resistant strains, thereby necessitating the discovery of new drugs. In this study, we found aprotinin, a serine protease inhibitor, as an anti-influenza candidate through screening of compound libraries. Aprotinin has been previously reported to show inhibitory effects on a few influenza A virus (IAV) subtypes (e.g., seasonal H1N1 and H3N2). However, because there were no reports of its inhibitory effects on the other types of influenza viruses, we investigated the inhibitory effects of aprotinin in vitro on a wide range of influenza viruses, including avian and oseltamivir-resistant influenza virus strains. Our cell-based assay showed that aprotinin had inhibitory effects on seasonal human IAVs (H1N1 and H3N2 subtypes), avian IAVs (H5N2, H6N5, and H9N2 subtypes), an oseltamivir-resistant IAV, and a currently circulating influenza B virus. We have also confirmed its activity in mice infected with a lethal dose of influenza virus, showing a significant increase in survival rate. Our findings suggest that aprotinin has the capacity to inhibit a wide range of influenza virus subtypes and should be considered for development as a therapeutic agent against influenza.
format	article
author	Eun-Jung Song Erica Españo Sang-Mu Shim Jeong-Hyun Nam Jiyeon Kim Kiho Lee Song-Kyu Park Chong-Kil Lee Jeong-Ki Kim
author_facet	Eun-Jung Song Erica Españo Sang-Mu Shim Jeong-Hyun Nam Jiyeon Kim Kiho Lee Song-Kyu Park Chong-Kil Lee Jeong-Ki Kim
author_sort	Eun-Jung Song
title	Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo
title_short	Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo
title_full	Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo
title_fullStr	Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo
title_full_unstemmed	Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo
title_sort	inhibitory effects of aprotinin on influenza a and b viruses in vitro and in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ad383a25f37f466e93110e0f247e0848
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Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo

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