Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination

Abstract Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules of mumps virus (MuV)-infected...

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Autores principales:	Patricia Kaaijk, Maarten E. Emmelot, Hugo D. Meiring, Cécile A. C. M. van Els, Jelle de Wit
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad3e330a48ac4d4a95ab13fdc8ebf73c
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spelling	oai:doaj.org-article:ad3e330a48ac4d4a95ab13fdc8ebf73c2021-12-02T16:10:37ZNovel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination10.1038/s41598-021-92926-12045-2322https://doaj.org/article/ad3e330a48ac4d4a95ab13fdc8ebf73c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92926-1https://doaj.org/toc/2045-2322Abstract Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules of mumps virus (MuV)-infected cells. Here, we confirmed immunogenicity of five novel HLA-B07:02- and HLA-A01:01-restricted MuV T cell epitopes from this set of peptides. High frequencies of T cells against these five MuV epitopes could be detected ex vivo in all tested mumps patients. Moreover, these epitope-specific T cells derived from mumps patients displayed strong cytotoxic activity. In contrast, only marginal T cell responses against these novel MuV epitopes could be detected in recently vaccinated persons, corroborating earlier findings. Identifying which MuV epitopes are dominantly targeted in the mumps-specific CD8+ T- response is an important step towards better understanding in the discrepancies between natural infection or vaccination-induced cell-mediated immune protection.Patricia KaaijkMaarten E. EmmelotHugo D. MeiringCécile A. C. M. van ElsJelle de WitNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patricia Kaaijk Maarten E. Emmelot Hugo D. Meiring Cécile A. C. M. van Els Jelle de Wit Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination
description	Abstract Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules of mumps virus (MuV)-infected cells. Here, we confirmed immunogenicity of five novel HLA-B07:02- and HLA-A01:01-restricted MuV T cell epitopes from this set of peptides. High frequencies of T cells against these five MuV epitopes could be detected ex vivo in all tested mumps patients. Moreover, these epitope-specific T cells derived from mumps patients displayed strong cytotoxic activity. In contrast, only marginal T cell responses against these novel MuV epitopes could be detected in recently vaccinated persons, corroborating earlier findings. Identifying which MuV epitopes are dominantly targeted in the mumps-specific CD8+ T- response is an important step towards better understanding in the discrepancies between natural infection or vaccination-induced cell-mediated immune protection.
format	article
author	Patricia Kaaijk Maarten E. Emmelot Hugo D. Meiring Cécile A. C. M. van Els Jelle de Wit
author_facet	Patricia Kaaijk Maarten E. Emmelot Hugo D. Meiring Cécile A. C. M. van Els Jelle de Wit
author_sort	Patricia Kaaijk
title	Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination
title_short	Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination
title_full	Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination
title_fullStr	Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination
title_full_unstemmed	Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination
title_sort	novel mumps virus epitopes reveal robust cytotoxic t cell responses after natural infection but not after vaccination
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ad3e330a48ac4d4a95ab13fdc8ebf73c
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Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination

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