Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.

<h4>Background</h4>GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the brain, is synthesized by glutamic acid decarboxylase (GAD). GAD exists in two adult isoforms, GAD65 and GAD67. During embryonic brain development at least two additional transcripts exist, I-80...

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Autores principales:	Anke Popp, Anja Urbach, Otto W Witte, Christiane Frahm
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad45c2fe3afe4f98ac5d01d19bd4101e
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spelling	oai:doaj.org-article:ad45c2fe3afe4f98ac5d01d19bd4101e2021-11-25T06:17:31ZAdult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.1932-620310.1371/journal.pone.0004371https://doaj.org/article/ad45c2fe3afe4f98ac5d01d19bd4101e2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19190758/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the brain, is synthesized by glutamic acid decarboxylase (GAD). GAD exists in two adult isoforms, GAD65 and GAD67. During embryonic brain development at least two additional transcripts exist, I-80 and I-86, which are distinguished by insertions of 80 or 86 bp into GAD67 mRNA, respectively. Though it was described that embryonic GAD67 transcripts are not detectable during adulthood there are evidences suggesting re-expression under certain pathological conditions in the adult brain. In the present study we systematically analyzed for the first time the spatiotemporal distribution of different GADs with emphasis on embryonic GAD67 mRNAs in the postnatal brain using highly sensitive methods.<h4>Methodology/principal findings</h4>QPCR was used to precisely investigate the postnatal expression level of GAD related mRNAs in cortex, hippocampus, cerebellum, and olfactory bulb of rats from P1 throughout adulthood. Within the first three postnatal weeks the expression of both GAD65 and GAD67 mRNAs reached adult levels in hippocampus, cortex, and cerebellum. The olfactory bulb showed by far the highest expression of GAD65 as well as GAD67 transcripts. Embryonic GAD67 splice variants were still detectable at birth. They continuously declined to barely detectable levels during postnatal development in all investigated regions with exception of a comparatively high expression in the olfactory bulb. Radioactive in situ hybridizations confirmed the occurrence of embryonic GAD67 transcripts in the olfactory bulb and furthermore detected their localization mainly in the subventricular zone and the rostral migratory stream.<h4>Conclusions/significance</h4>Embryonic GAD67 transcripts can hardly be detected in the adult brain, except for specific regions associated with neurogenesis and high synaptic plasticity. Therefore a functional role in processes like proliferation, migration or synaptogenesis is suggested.Anke PoppAnja UrbachOtto W WitteChristiane FrahmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4371 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anke Popp Anja Urbach Otto W Witte Christiane Frahm Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.
description	<h4>Background</h4>GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the brain, is synthesized by glutamic acid decarboxylase (GAD). GAD exists in two adult isoforms, GAD65 and GAD67. During embryonic brain development at least two additional transcripts exist, I-80 and I-86, which are distinguished by insertions of 80 or 86 bp into GAD67 mRNA, respectively. Though it was described that embryonic GAD67 transcripts are not detectable during adulthood there are evidences suggesting re-expression under certain pathological conditions in the adult brain. In the present study we systematically analyzed for the first time the spatiotemporal distribution of different GADs with emphasis on embryonic GAD67 mRNAs in the postnatal brain using highly sensitive methods.<h4>Methodology/principal findings</h4>QPCR was used to precisely investigate the postnatal expression level of GAD related mRNAs in cortex, hippocampus, cerebellum, and olfactory bulb of rats from P1 throughout adulthood. Within the first three postnatal weeks the expression of both GAD65 and GAD67 mRNAs reached adult levels in hippocampus, cortex, and cerebellum. The olfactory bulb showed by far the highest expression of GAD65 as well as GAD67 transcripts. Embryonic GAD67 splice variants were still detectable at birth. They continuously declined to barely detectable levels during postnatal development in all investigated regions with exception of a comparatively high expression in the olfactory bulb. Radioactive in situ hybridizations confirmed the occurrence of embryonic GAD67 transcripts in the olfactory bulb and furthermore detected their localization mainly in the subventricular zone and the rostral migratory stream.<h4>Conclusions/significance</h4>Embryonic GAD67 transcripts can hardly be detected in the adult brain, except for specific regions associated with neurogenesis and high synaptic plasticity. Therefore a functional role in processes like proliferation, migration or synaptogenesis is suggested.
format	article
author	Anke Popp Anja Urbach Otto W Witte Christiane Frahm
author_facet	Anke Popp Anja Urbach Otto W Witte Christiane Frahm
author_sort	Anke Popp
title	Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.
title_short	Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.
title_full	Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.
title_fullStr	Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.
title_full_unstemmed	Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.
title_sort	adult and embryonic gad transcripts are spatiotemporally regulated during postnatal development in the rat brain.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/ad45c2fe3afe4f98ac5d01d19bd4101e
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Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.

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