Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations
Abstract Accumulating evidence supports a role of the PI3K-AKT pathway in the regulation of cell motility, invasion and metastasis. AKT activation is known to promote metastasis, however under certain circumstances, it also shows an inhibitory activity on metastatic processes, and the cause of such...
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2017
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oai:doaj.org-article:ad4629a1aed346e99c610373d11cbf2a2021-12-02T11:41:20ZInhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations10.1038/s41598-017-06128-92045-2322https://doaj.org/article/ad4629a1aed346e99c610373d11cbf2a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06128-9https://doaj.org/toc/2045-2322Abstract Accumulating evidence supports a role of the PI3K-AKT pathway in the regulation of cell motility, invasion and metastasis. AKT activation is known to promote metastasis, however under certain circumstances, it also shows an inhibitory activity on metastatic processes, and the cause of such conflicting results is largely unclear. Here we found that AKT1 is an important regulator of metastasis and down-regulation of its activity is associated with increased metastatic potential of A549 cells. Inhibition of AKT1 enhanced migration and invasion in KRAS- or EGFR-mutant non-small cell lung cancer (NSCLC) cells. The allosteric AKT inhibitor MK-2206 promoted metastasis of KRAS-mutated A549 cells in vivo. We next identified that the phosphorylation of Myristoylated alanine-rich C-kinase substrate (MARCKS) and LAMC2 protein level were increased with AKT1 inhibition, and MARCKS or LAMC2 knockdown abrogated migration and invasion induced by AKT1 inhibition. This study unravels an anti-metastatic role of AKT1 in the NSCLC cells with KRAS or EGFR mutations, and establishes an AKT1-MARCKS-LAMC2 feedback loop in this regulation.Guanhua RaoMariaelena PierobonIn-Kyu KimWei-Hsun HsuJianghong DengYong-Wha MoonEmanuel F. PetricoinYu-Wen ZhangYisong WangGiuseppe GiacconeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Guanhua Rao Mariaelena Pierobon In-Kyu Kim Wei-Hsun Hsu Jianghong Deng Yong-Wha Moon Emanuel F. Petricoin Yu-Wen Zhang Yisong Wang Giuseppe Giaccone Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations |
| description |
Abstract Accumulating evidence supports a role of the PI3K-AKT pathway in the regulation of cell motility, invasion and metastasis. AKT activation is known to promote metastasis, however under certain circumstances, it also shows an inhibitory activity on metastatic processes, and the cause of such conflicting results is largely unclear. Here we found that AKT1 is an important regulator of metastasis and down-regulation of its activity is associated with increased metastatic potential of A549 cells. Inhibition of AKT1 enhanced migration and invasion in KRAS- or EGFR-mutant non-small cell lung cancer (NSCLC) cells. The allosteric AKT inhibitor MK-2206 promoted metastasis of KRAS-mutated A549 cells in vivo. We next identified that the phosphorylation of Myristoylated alanine-rich C-kinase substrate (MARCKS) and LAMC2 protein level were increased with AKT1 inhibition, and MARCKS or LAMC2 knockdown abrogated migration and invasion induced by AKT1 inhibition. This study unravels an anti-metastatic role of AKT1 in the NSCLC cells with KRAS or EGFR mutations, and establishes an AKT1-MARCKS-LAMC2 feedback loop in this regulation. |
| format |
article |
| author |
Guanhua Rao Mariaelena Pierobon In-Kyu Kim Wei-Hsun Hsu Jianghong Deng Yong-Wha Moon Emanuel F. Petricoin Yu-Wen Zhang Yisong Wang Giuseppe Giaccone |
| author_facet |
Guanhua Rao Mariaelena Pierobon In-Kyu Kim Wei-Hsun Hsu Jianghong Deng Yong-Wha Moon Emanuel F. Petricoin Yu-Wen Zhang Yisong Wang Giuseppe Giaccone |
| author_sort |
Guanhua Rao |
| title |
Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations |
| title_short |
Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations |
| title_full |
Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations |
| title_fullStr |
Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations |
| title_full_unstemmed |
Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations |
| title_sort |
inhibition of akt1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with k-ras or egfr mutations |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/ad4629a1aed346e99c610373d11cbf2a |
| work_keys_str_mv |
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