Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice

Here, the authors show that sequential treatment with long-acting slow-effective release ART and AAV9- based delivery of CRISPR-Cas9 results in undetectable levels of virus and integrated DNA in a subset of humanized HIV-1 infected mice. This proof-of-concept study suggests that HIV-1 elimination is...

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Autores principales:	Prasanta K. Dash, Rafal Kaminski, Ramona Bella, Hang Su, Saumi Mathews, Taha M. Ahooyi, Chen Chen, Pietro Mancuso, Rahsan Sariyer, Pasquale Ferrante, Martina Donadoni, Jake A. Robinson, Brady Sillman, Zhiyi Lin, James R. Hilaire, Mary Banoub, Monalisha Elango, Nagsen Gautam, R. Lee Mosley, Larisa Y. Poluektova, JoEllyn McMillan, Aditya N. Bade, Santhi Gorantla, Ilker K. Sariyer, Tricia H. Burdo, Won-Bin Young, Shohreh Amini, Jennifer Gordon, Jeffrey M. Jacobson, Benson Edagwa, Kamel Khalili, Howard E. Gendelman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ad4b010214414d8c84777d8ae90f684b
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spelling	oai:doaj.org-article:ad4b010214414d8c84777d8ae90f684b2021-12-02T14:39:56ZSequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice10.1038/s41467-019-10366-y2041-1723https://doaj.org/article/ad4b010214414d8c84777d8ae90f684b2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10366-yhttps://doaj.org/toc/2041-1723Here, the authors show that sequential treatment with long-acting slow-effective release ART and AAV9- based delivery of CRISPR-Cas9 results in undetectable levels of virus and integrated DNA in a subset of humanized HIV-1 infected mice. This proof-of-concept study suggests that HIV-1 elimination is possible.Prasanta K. DashRafal KaminskiRamona BellaHang SuSaumi MathewsTaha M. AhooyiChen ChenPietro MancusoRahsan SariyerPasquale FerranteMartina DonadoniJake A. RobinsonBrady SillmanZhiyi LinJames R. HilaireMary BanoubMonalisha ElangoNagsen GautamR. Lee MosleyLarisa Y. PoluektovaJoEllyn McMillanAditya N. BadeSanthi GorantlaIlker K. SariyerTricia H. BurdoWon-Bin YoungShohreh AminiJennifer GordonJeffrey M. JacobsonBenson EdagwaKamel KhaliliHoward E. GendelmanNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-20 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Prasanta K. Dash Rafal Kaminski Ramona Bella Hang Su Saumi Mathews Taha M. Ahooyi Chen Chen Pietro Mancuso Rahsan Sariyer Pasquale Ferrante Martina Donadoni Jake A. Robinson Brady Sillman Zhiyi Lin James R. Hilaire Mary Banoub Monalisha Elango Nagsen Gautam R. Lee Mosley Larisa Y. Poluektova JoEllyn McMillan Aditya N. Bade Santhi Gorantla Ilker K. Sariyer Tricia H. Burdo Won-Bin Young Shohreh Amini Jennifer Gordon Jeffrey M. Jacobson Benson Edagwa Kamel Khalili Howard E. Gendelman Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
description	Here, the authors show that sequential treatment with long-acting slow-effective release ART and AAV9- based delivery of CRISPR-Cas9 results in undetectable levels of virus and integrated DNA in a subset of humanized HIV-1 infected mice. This proof-of-concept study suggests that HIV-1 elimination is possible.
format	article
author	Prasanta K. Dash Rafal Kaminski Ramona Bella Hang Su Saumi Mathews Taha M. Ahooyi Chen Chen Pietro Mancuso Rahsan Sariyer Pasquale Ferrante Martina Donadoni Jake A. Robinson Brady Sillman Zhiyi Lin James R. Hilaire Mary Banoub Monalisha Elango Nagsen Gautam R. Lee Mosley Larisa Y. Poluektova JoEllyn McMillan Aditya N. Bade Santhi Gorantla Ilker K. Sariyer Tricia H. Burdo Won-Bin Young Shohreh Amini Jennifer Gordon Jeffrey M. Jacobson Benson Edagwa Kamel Khalili Howard E. Gendelman
author_facet	Prasanta K. Dash Rafal Kaminski Ramona Bella Hang Su Saumi Mathews Taha M. Ahooyi Chen Chen Pietro Mancuso Rahsan Sariyer Pasquale Ferrante Martina Donadoni Jake A. Robinson Brady Sillman Zhiyi Lin James R. Hilaire Mary Banoub Monalisha Elango Nagsen Gautam R. Lee Mosley Larisa Y. Poluektova JoEllyn McMillan Aditya N. Bade Santhi Gorantla Ilker K. Sariyer Tricia H. Burdo Won-Bin Young Shohreh Amini Jennifer Gordon Jeffrey M. Jacobson Benson Edagwa Kamel Khalili Howard E. Gendelman
author_sort	Prasanta K. Dash
title	Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
title_short	Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
title_full	Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
title_fullStr	Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
title_full_unstemmed	Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
title_sort	sequential laser art and crispr treatments eliminate hiv-1 in a subset of infected humanized mice
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ad4b010214414d8c84777d8ae90f684b
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Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice

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