Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

The germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic n...

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Autores principales: Ian F Price, Hannah L Hertz, Benjamin Pastore, Jillian Wagner, Wen Tang
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Publicado: eLife Sciences Publications Ltd 2021
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Acceso en línea:https://doaj.org/article/ad508ac3e75344e7a723ea8b0a27b2a8
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spelling oai:doaj.org-article:ad508ac3e75344e7a723ea8b0a27b2a82021-12-02T15:20:56ZProximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans10.7554/eLife.722762050-084Xe72276https://doaj.org/article/ad508ac3e75344e7a723ea8b0a27b2a82021-11-01T00:00:00Zhttps://elifesciences.org/articles/72276https://doaj.org/toc/2050-084XThe germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic nature and their exclusive expression in the germ line. Using Caenorhabditis elegans germ granule, called P granule, as a model system, we employed a proximity-based labeling method in combination with mass spectrometry to comprehensively define its protein components. This set of experiments identified over 200 proteins, many of which contain intrinsically disordered regions (IDRs). An RNA interference-based screen identified factors that are essential for P granule assembly, notably EGGD-1 and EGGD-2, two putative LOTUS-domain proteins. Loss of eggd-1 and eggd-2 results in separation of P granules from the nuclear envelope, germline atrophy, and reduced fertility. We show that IDRs of EGGD-1 are required to anchor EGGD-1 to the nuclear periphery while its LOTUS domains are required to promote the perinuclear localization of P granules. Taken together, our work expands the repertoire of P granule constituents and provides new insights into the role of LOTUS-domain proteins in germ granule organization.Ian F PriceHannah L HertzBenjamin PastoreJillian WagnerWen TangeLife Sciences Publications Ltdarticlegerm granulesLOTUS domain proteinproximity labelingp granulesvasa proteinsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution DOAJ
collection DOAJ
language EN
topic germ granules
LOTUS domain protein
proximity labeling
p granules
vasa proteins
Medicine
R
Science
Q
Biology (General)
QH301-705.5
spellingShingle germ granules
LOTUS domain protein
proximity labeling
p granules
vasa proteins
Medicine
R
Science
Q
Biology (General)
QH301-705.5
Ian F Price
Hannah L Hertz
Benjamin Pastore
Jillian Wagner
Wen Tang
Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
description The germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic nature and their exclusive expression in the germ line. Using Caenorhabditis elegans germ granule, called P granule, as a model system, we employed a proximity-based labeling method in combination with mass spectrometry to comprehensively define its protein components. This set of experiments identified over 200 proteins, many of which contain intrinsically disordered regions (IDRs). An RNA interference-based screen identified factors that are essential for P granule assembly, notably EGGD-1 and EGGD-2, two putative LOTUS-domain proteins. Loss of eggd-1 and eggd-2 results in separation of P granules from the nuclear envelope, germline atrophy, and reduced fertility. We show that IDRs of EGGD-1 are required to anchor EGGD-1 to the nuclear periphery while its LOTUS domains are required to promote the perinuclear localization of P granules. Taken together, our work expands the repertoire of P granule constituents and provides new insights into the role of LOTUS-domain proteins in germ granule organization.
format article
author Ian F Price
Hannah L Hertz
Benjamin Pastore
Jillian Wagner
Wen Tang
author_facet Ian F Price
Hannah L Hertz
Benjamin Pastore
Jillian Wagner
Wen Tang
author_sort Ian F Price
title Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_short Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_full Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_fullStr Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_full_unstemmed Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_sort proximity labeling identifies lotus domain proteins that promote the formation of perinuclear germ granules in c. elegans
publisher eLife Sciences Publications Ltd
publishDate 2021
url https://doaj.org/article/ad508ac3e75344e7a723ea8b0a27b2a8
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AT benjaminpastore proximitylabelingidentifieslotusdomainproteinsthatpromotetheformationofperinucleargermgranulesincelegans
AT jillianwagner proximitylabelingidentifieslotusdomainproteinsthatpromotetheformationofperinucleargermgranulesincelegans
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