Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

The germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic n...

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Autores principales:	Ian F Price, Hannah L Hertz, Benjamin Pastore, Jillian Wagner, Wen Tang
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	germ granules LOTUS domain protein proximity labeling p granules vasa proteins Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ad508ac3e75344e7a723ea8b0a27b2a8
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spelling	oai:doaj.org-article:ad508ac3e75344e7a723ea8b0a27b2a82021-12-02T15:20:56ZProximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans10.7554/eLife.722762050-084Xe72276https://doaj.org/article/ad508ac3e75344e7a723ea8b0a27b2a82021-11-01T00:00:00Zhttps://elifesciences.org/articles/72276https://doaj.org/toc/2050-084XThe germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic nature and their exclusive expression in the germ line. Using Caenorhabditis elegans germ granule, called P granule, as a model system, we employed a proximity-based labeling method in combination with mass spectrometry to comprehensively define its protein components. This set of experiments identified over 200 proteins, many of which contain intrinsically disordered regions (IDRs). An RNA interference-based screen identified factors that are essential for P granule assembly, notably EGGD-1 and EGGD-2, two putative LOTUS-domain proteins. Loss of eggd-1 and eggd-2 results in separation of P granules from the nuclear envelope, germline atrophy, and reduced fertility. We show that IDRs of EGGD-1 are required to anchor EGGD-1 to the nuclear periphery while its LOTUS domains are required to promote the perinuclear localization of P granules. Taken together, our work expands the repertoire of P granule constituents and provides new insights into the role of LOTUS-domain proteins in germ granule organization.Ian F PriceHannah L HertzBenjamin PastoreJillian WagnerWen TangeLife Sciences Publications Ltdarticlegerm granulesLOTUS domain proteinproximity labelingp granulesvasa proteinsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	germ granules LOTUS domain protein proximity labeling p granules vasa proteins Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	germ granules LOTUS domain protein proximity labeling p granules vasa proteins Medicine R Science Q Biology (General) QH301-705.5 Ian F Price Hannah L Hertz Benjamin Pastore Jillian Wagner Wen Tang Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
description	The germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules—well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic nature and their exclusive expression in the germ line. Using Caenorhabditis elegans germ granule, called P granule, as a model system, we employed a proximity-based labeling method in combination with mass spectrometry to comprehensively define its protein components. This set of experiments identified over 200 proteins, many of which contain intrinsically disordered regions (IDRs). An RNA interference-based screen identified factors that are essential for P granule assembly, notably EGGD-1 and EGGD-2, two putative LOTUS-domain proteins. Loss of eggd-1 and eggd-2 results in separation of P granules from the nuclear envelope, germline atrophy, and reduced fertility. We show that IDRs of EGGD-1 are required to anchor EGGD-1 to the nuclear periphery while its LOTUS domains are required to promote the perinuclear localization of P granules. Taken together, our work expands the repertoire of P granule constituents and provides new insights into the role of LOTUS-domain proteins in germ granule organization.
format	article
author	Ian F Price Hannah L Hertz Benjamin Pastore Jillian Wagner Wen Tang
author_facet	Ian F Price Hannah L Hertz Benjamin Pastore Jillian Wagner Wen Tang
author_sort	Ian F Price
title	Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_short	Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_full	Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_fullStr	Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_full_unstemmed	Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
title_sort	proximity labeling identifies lotus domain proteins that promote the formation of perinuclear germ granules in c. elegans
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/ad508ac3e75344e7a723ea8b0a27b2a8
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Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

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