Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity

Blaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) we...

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Autores principales:	Wang Ying, Yang Yin-He, Luo Qing, Hu Yuan, Lu Qian, Yu Wan-Xin, Chen Jin-Hu, Wang Yu-Jia, Wang Mu-Ling, Zhao Yu, Xiao Huai
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	blaps rynchopetera polysaccharides content determination monosaccharide composition macrophage phagocytic activity Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ad58237692494a40b9c1ef37c9a1f1ae
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spelling	oai:doaj.org-article:ad58237692494a40b9c1ef37c9a1f1ae2021-12-05T14:10:43ZContent and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity2391-542010.1515/chem-2021-0035https://doaj.org/article/ad58237692494a40b9c1ef37c9a1f1ae2021-03-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0035https://doaj.org/toc/2391-5420Blaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) were reported. B. rhynchoptera is rich in polysaccharides (content value 3.97%). Through PMP (1-phenyl-3-methyl-5-pyrazolone) pre-column derivatization and high performance liquid chromatography (HPLC) testing, the results showed that RPS consist of 8 known monosaccharides, including D-mannose (Man), Rhamnose (Rha), D-glucuronic acid (GlcUA), D-galacturonic acid (GalUA), D-glucose (Glc), D-galactose (Gal), Arabinose (Ara), and Fucose (Fuc), with the total content of 171.70 mg g−1 and Glc has the highest content of 45.40 mg g−1. The phagocytic ability of mouse peritoneal macrophages was investigated after RPS stimulating alone and combined with lipopolysaccharide (LPS). RPS played an important role in the engulfment of mouse peritoneal macrophages and can significantly enhance the phagocytic ability of macrophages. However, no synergistic effects were observed when RPS combined with LPS.Wang YingYang Yin-HeLuo QingHu YuanLu QianYu Wan-XinChen Jin-HuWang Yu-JiaWang Mu-LingZhao YuXiao HuaiDe Gruyterarticleblaps rynchopeterapolysaccharidescontent determinationmonosaccharide compositionmacrophage phagocytic activityChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 338-346 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	blaps rynchopetera polysaccharides content determination monosaccharide composition macrophage phagocytic activity Chemistry QD1-999
spellingShingle	blaps rynchopetera polysaccharides content determination monosaccharide composition macrophage phagocytic activity Chemistry QD1-999 Wang Ying Yang Yin-He Luo Qing Hu Yuan Lu Qian Yu Wan-Xin Chen Jin-Hu Wang Yu-Jia Wang Mu-Ling Zhao Yu Xiao Huai Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
description	Blaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) were reported. B. rhynchoptera is rich in polysaccharides (content value 3.97%). Through PMP (1-phenyl-3-methyl-5-pyrazolone) pre-column derivatization and high performance liquid chromatography (HPLC) testing, the results showed that RPS consist of 8 known monosaccharides, including D-mannose (Man), Rhamnose (Rha), D-glucuronic acid (GlcUA), D-galacturonic acid (GalUA), D-glucose (Glc), D-galactose (Gal), Arabinose (Ara), and Fucose (Fuc), with the total content of 171.70 mg g−1 and Glc has the highest content of 45.40 mg g−1. The phagocytic ability of mouse peritoneal macrophages was investigated after RPS stimulating alone and combined with lipopolysaccharide (LPS). RPS played an important role in the engulfment of mouse peritoneal macrophages and can significantly enhance the phagocytic ability of macrophages. However, no synergistic effects were observed when RPS combined with LPS.
format	article
author	Wang Ying Yang Yin-He Luo Qing Hu Yuan Lu Qian Yu Wan-Xin Chen Jin-Hu Wang Yu-Jia Wang Mu-Ling Zhao Yu Xiao Huai
author_facet	Wang Ying Yang Yin-He Luo Qing Hu Yuan Lu Qian Yu Wan-Xin Chen Jin-Hu Wang Yu-Jia Wang Mu-Ling Zhao Yu Xiao Huai
author_sort	Wang Ying
title	Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
title_short	Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
title_full	Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
title_fullStr	Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
title_full_unstemmed	Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
title_sort	content and composition analysis of polysaccharides from blaps rynchopetera and its macrophage phagocytic activity
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/ad58237692494a40b9c1ef37c9a1f1ae
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Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity

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