<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated

Non-tuberculous mycobacteria (NTM) are opportunistic pathogens that cause illness primarily in the elderly, in the immunocompromised or in patients with underlying lung disease. Since 2013, a global outbreak of NTM infection related to heater-cooler units (HCU) used in cardio-thoracic surgery has be...

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Autores principales:	Mor Rubinstein, Rona Grossman, Israel Nissan, Mitchell J. Schwaber, Yehuda Carmeli, Hasia Kaidar-Shwartz, Zeev Dveyrin, Efrat Rorman
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Mycobacterium chimaera</i> NTM metagenomic binning Medicine R
Acceso en línea:	https://doaj.org/article/ad59711795b34a5390c8ff1f0f2ebdba
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spelling	oai:doaj.org-article:ad59711795b34a5390c8ff1f0f2ebdba2021-11-25T18:37:52Z<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated10.3390/pathogens101113922076-0817https://doaj.org/article/ad59711795b34a5390c8ff1f0f2ebdba2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1392https://doaj.org/toc/2076-0817Non-tuberculous mycobacteria (NTM) are opportunistic pathogens that cause illness primarily in the elderly, in the immunocompromised or in patients with underlying lung disease. Since 2013, a global outbreak of NTM infection related to heater-cooler units (HCU) used in cardio-thoracic surgery has been identified. This outbreak was caused by a single strain of <i>Mycobacterium intracellulare subsp. chimaera</i>. In order to estimate the prevalence of this outbreak strain in Israel, we sampled <i>Mycobacterium intracellulare subsp. chimaera</i> from several HCU machines in Israel, as well as from patients, sequenced their genomes and compared them to the outbreak strain. The presence of mixed mycobacteria species in the samples complicated the analysis of obtained sequences. By applying a metagenomic binning strategy, we were able to obtain, and characterize, genomes of single strains from the mixed samples. <i>Mycobacterium intracellulare subsp. chimaera</i> strains were compared to each other and to previously reported genomes from other countries. The strain causing the outbreak related to the HCU machines was identified in several such machines in Israel but not in any clinical sample.Mor RubinsteinRona GrossmanIsrael NissanMitchell J. SchwaberYehuda CarmeliHasia Kaidar-ShwartzZeev DveyrinEfrat RormanMDPI AGarticle<i>Mycobacterium chimaera</i>NTMmetagenomic binningMedicineRENPathogens, Vol 10, Iss 1392, p 1392 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Mycobacterium chimaera</i> NTM metagenomic binning Medicine R
spellingShingle	<i>Mycobacterium chimaera</i> NTM metagenomic binning Medicine R Mor Rubinstein Rona Grossman Israel Nissan Mitchell J. Schwaber Yehuda Carmeli Hasia Kaidar-Shwartz Zeev Dveyrin Efrat Rorman <i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated
description	Non-tuberculous mycobacteria (NTM) are opportunistic pathogens that cause illness primarily in the elderly, in the immunocompromised or in patients with underlying lung disease. Since 2013, a global outbreak of NTM infection related to heater-cooler units (HCU) used in cardio-thoracic surgery has been identified. This outbreak was caused by a single strain of <i>Mycobacterium intracellulare subsp. chimaera</i>. In order to estimate the prevalence of this outbreak strain in Israel, we sampled <i>Mycobacterium intracellulare subsp. chimaera</i> from several HCU machines in Israel, as well as from patients, sequenced their genomes and compared them to the outbreak strain. The presence of mixed mycobacteria species in the samples complicated the analysis of obtained sequences. By applying a metagenomic binning strategy, we were able to obtain, and characterize, genomes of single strains from the mixed samples. <i>Mycobacterium intracellulare subsp. chimaera</i> strains were compared to each other and to previously reported genomes from other countries. The strain causing the outbreak related to the HCU machines was identified in several such machines in Israel but not in any clinical sample.
format	article
author	Mor Rubinstein Rona Grossman Israel Nissan Mitchell J. Schwaber Yehuda Carmeli Hasia Kaidar-Shwartz Zeev Dveyrin Efrat Rorman
author_facet	Mor Rubinstein Rona Grossman Israel Nissan Mitchell J. Schwaber Yehuda Carmeli Hasia Kaidar-Shwartz Zeev Dveyrin Efrat Rorman
author_sort	Mor Rubinstein
title	<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated
title_short	<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated
title_full	<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated
title_fullStr	<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated
title_full_unstemmed	<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated
title_sort	<i>mycobacterium intracellulare subsp. chimaera</i> from cardio surgery heating-cooling units and from clinical samples in israel are genetically unrelated
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ad59711795b34a5390c8ff1f0f2ebdba
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<i>Mycobacterium intracellulare subsp. chimaera</i> from Cardio Surgery Heating-Cooling Units and from Clinical Samples in Israel Are Genetically Unrelated

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