Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection

In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are p...

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Autores principales:	Hafiz Zeeshan Mahmood, Asim Jilani, Sajid Farooq, Yasir Javed, Yasir Jamil, Javed Iqbal, Sami Ullah, Swelm Wageh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	localized surface plasmon resonance gold nanoparticle plasmonic sensor dengue detection Crystallography QD901-999
Acceso en línea:	https://doaj.org/article/ad61d227129648449b36e936392d755e
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id	oai:doaj.org-article:ad61d227129648449b36e936392d755e
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spelling	oai:doaj.org-article:ad61d227129648449b36e936392d755e2021-11-25T17:18:26ZPlasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection10.3390/cryst111113402073-4352https://doaj.org/article/ad61d227129648449b36e936392d755e2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4352/11/11/1340https://doaj.org/toc/2073-4352In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are performed to assess the LSPR extinction spectrum and spatial near electric field distribution around the nanoparticle surface. Important parameters that govern sensor performance, molecular and refractive index sensitivity are evaluated. On the evaluation of the platform as a molecular sensor, the detection of dengue NS1 antigens is presented. The results are consistent with the numerical simulations, which depicts the system’s ability to identify dengue NS1 antigen concentrations as low as 0.07 ± 0.01 µg/mL, along with fosters its potential application in plasmonic sensing.Hafiz Zeeshan MahmoodAsim JilaniSajid FarooqYasir JavedYasir JamilJaved IqbalSami UllahSwelm WagehMDPI AGarticlelocalized surface plasmon resonancegold nanoparticleplasmonic sensordengue detectionCrystallographyQD901-999ENCrystals, Vol 11, Iss 1340, p 1340 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	localized surface plasmon resonance gold nanoparticle plasmonic sensor dengue detection Crystallography QD901-999
spellingShingle	localized surface plasmon resonance gold nanoparticle plasmonic sensor dengue detection Crystallography QD901-999 Hafiz Zeeshan Mahmood Asim Jilani Sajid Farooq Yasir Javed Yasir Jamil Javed Iqbal Sami Ullah Swelm Wageh Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
description	In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are performed to assess the LSPR extinction spectrum and spatial near electric field distribution around the nanoparticle surface. Important parameters that govern sensor performance, molecular and refractive index sensitivity are evaluated. On the evaluation of the platform as a molecular sensor, the detection of dengue NS1 antigens is presented. The results are consistent with the numerical simulations, which depicts the system’s ability to identify dengue NS1 antigen concentrations as low as 0.07 ± 0.01 µg/mL, along with fosters its potential application in plasmonic sensing.
format	article
author	Hafiz Zeeshan Mahmood Asim Jilani Sajid Farooq Yasir Javed Yasir Jamil Javed Iqbal Sami Ullah Swelm Wageh
author_facet	Hafiz Zeeshan Mahmood Asim Jilani Sajid Farooq Yasir Javed Yasir Jamil Javed Iqbal Sami Ullah Swelm Wageh
author_sort	Hafiz Zeeshan Mahmood
title	Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
title_short	Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
title_full	Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
title_fullStr	Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
title_full_unstemmed	Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
title_sort	plasmon-based label-free biosensor using gold nanosphere for dengue detection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ad61d227129648449b36e936392d755e
work_keys_str_mv	AT hafizzeeshanmahmood plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT asimjilani plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT sajidfarooq plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT yasirjaved plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT yasirjamil plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT javediqbal plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT samiullah plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection AT swelmwageh plasmonbasedlabelfreebiosensorusinggoldnanospherefordenguedetection
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Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection

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